'98 Escherichia coli SWISS-2DPAGE database update

The combination of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), computer image analysis and several protein identification techniques allowed the Escherichia coli SWISS-2DPAGE database to be established. This is part of the ExPASy molecular biology server accessible through the WWW at the URL address http://www.expasy.ch/ch2d/ch2d-top.html . Here we report recent progress in the development of the E. coli SWISS-2DPAGE database. Proteins were separated with immobilized pH gradients in the first dimension and sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. To increase the resolution of the separation and thus the number of identified proteins, a variety of wide and narrow range immobilized pH gradients were used in the first dimension. Micropreparative gels were electroblotted onto polyvinylidene difluoride membranes and spots were visualized by amido black staining. Protein identification techniques such as amino acid composition analysis, gel comparison and microsequencing were used, as well as a recently described Edman "sequence tag" approach. Some of the above identification techniques were coupled with database searching tools. Currently 231 polypeptides are identified on the E. coli SWISS-2DPAGE map: 64 have been identified by N-terminal microsequencing, 39 by amino acid composition, and 82 by sequence tag. Of 153 proteins putatively identified by gel comparison, 65 have been confirmed. Many proteins have been identified using more than one technique. Faster progress in the E. coli proteome project will now be possible with advances in biochemical methodology and with the completion of the entire E. coli genome.     

Speech perception performance in experienced cochlear-implant patients receiving the SPEAK processing strategy in the Nucleus Spectra-22 cochlear implant

Sixteen experienced cochlear implant patients with a wide range of speech-perception abilities received the SPEAK processing strategy in the Nucleus Spectra-22 cochlear implant. Speech perception was assessed in quiet and in noise with SPEAK and with the patients' previous strategies (for most, Multipeak) at the study onset, as well as after using SPEAK for 6 months. Comparisons were made within and across the two test sessions to elucidate possible learning effects. Patients were also asked to rate the strategies on seven speech recognition and sound quality scales. After 6 months' experience with SPEAK, patients showed significantly improved mean performance on a range of speech recognition measures in quiet and noise. When mean subjective ratings were compared over time there were no significant differences noted between strategies. However, many individuals rated the SPEAK strategy better for two or more of the seven subjective measures. Ratings for "appreciation of music" and "quality of my own voice" in particular were generally higher for SPEAK. Improvements were realized by patients with a wide range of speech perception abilities, including those with little or no open-set speech recognition.     

Spectrometer slit-power-coupling calculations for natural and laser guide-star adaptive optics

We use numerical calculations to examine the relation between adaptive optics (AO) turbulence compensation and power coupled through a spectrometer slit for both laser and natural guide-star AO systems. The AO system and observing parameters used are relevant to the Gemini-North 8-m telescope. For this study, we separate residual tilt from residual higher-order aberrations to isolate their relative effects under a variety of operating conditions. Our results demonstrate that slit-coupled intensity is not uniquely determined by system Strehl alone; we show that this is due to the differing effects of higher-order and tilt aberrations on the shape of the compensated point-spread function. For the Gemini spectrometer and AO system, the wider point-spread function halo associated with an added residual higher-order aberration reduces slit-coupled intensity more rapidly than a broad point-spread function core induced by residual tilt.     

An atomic force microscope study of surface roughness of thin silicon films deposited on SiO/sub 2/

Atomic force microscope analysis, with a resolution of /spl lsim/1.1 nm, shows that peak-to-peak surface roughness (/spl Delta/h/sub p-p/) of amorphous silicon films thinner than /spl ap/50 nm on silicon dioxide can be controlled to better than 5 nm. Low-pressure, chemically-vapor-deposited silicon films on silicon dioxide initially show an approximately linear increase in the surface roughness due to growing nuclei as the deposition progresses, followed by a decrease in the surface roughness as growth nuclei coalesce. A simple model based on random nucleation and nuclei growth displays similar trends. Films deposited on rougher substrates show more surface roughness. Surface treatment during the predeposition cleaning process does not significantly affect /spl Delta/h/sub p-p/. As a means of producing smooth surfaces, films thinner than about 20 nm are first deposited more thickly than needed, and then etched back to the desired dimension; the use of a binary HNO/sub 3/ and HF etching process improves roughness control. Boron-ion implanted and subsequently crystallized 45-nm-thick Si films show significant smoothing with /spl Delta/h/sub p-p//spl ap/2.2 nm. Thin amorphous silicon films deposited by source evaporation are attractive because they can be deposited at room temperature, and have smoother surfaces (/spl Delta/h/sub p-p//spl ap/2.5 nm) than comparable films produced by chemical vapor deposition.     

Mapping intertidal estuarine sediment grain size distributions through airborne remote sensing

The intertidal environments of estuaries represent critical exchange environments of sediment and sediment bound contaminants. Ecological and sedimentological related investigations of these environments require monitoring methods that provide rapid spatially representative data on sediment grain size distribution. Remote sensing has the potential to provide synoptic information of intertidal environments. Previous in situ and laboratory-based reflectance investigations have demonstrated that for effective quantification of sediment grain size distributions, remote sensing platforms must include measurements within the short-wave infrared (SWIR). In addition, the timing of image acquisition, in relation to tidal cycles and sediment moisture content, is critical in optimising the spectral differences between the coarser sand and finer ‘mud’ fraction of sediments. Daedalus 1268 Airborne Thematic Mapper (ATM) has been identified as an appropriate platform and sensor for providing accurate synoptic maps of estuarine sediment distributions. This paper presents the results from the application of ATM 1.75 m resolution data to the mapping of surface sediment grain-size distributions across intertidal areas of Ribble Estuary, Lancashire, UK. ATM imagery was acquired after the intertidal area was exposed to strong summer drying conditions. Pre-processing and linear unmixing of the imagery collected of the intertidal zone following a period of drying allowed accurate sub-pixel determinations (1.75 m resolution) of sediment clay (r²=0.79) but less accurate for sand (r²=0.60). The results also demonstrate deterioration in the image calibration with increasing sediment moisture content and microphytobenthos cover. However, recombining the subpixel end member abundances through multivariate regression analysis improved the image calibration significantly for both sediment clay and sand content (r²>0.8) for imagery collected in both dryer and wetter conditions. These results demonstrate that ATM data, or similar, can be used to gain quantitative information on intertidal sediment distributions and such data has application to a wide variety of estuarine research.     

Hydraulic resistance of fluids flowing in channels at supercritical pressures (survey)

This literature survey is devoted to hydraulic resistance of water and carbon dioxide flows at supercritical pressures. The objectives are to assess previous studies that were done in the area of hydraulic resistance of fluids at supercritical pressures flowing in channels of various geometries and to understand the specifics of pressure drop at these conditions. The literature search showed that the majority of experimental data were obtained in vertical tubes, some data were obtained in horizontal tubes and just a few of them in other flow geometries including bundles. In general, hydraulic resistance data are limited compared to the heat transfer data at supercritical pressures. Differences in pressure drop between supercritical and subcritical forced convection seem to be related to significant variations in thermophysical properties near the critical and pseudocritical points. Due to these variations, satisfactory analytical and numerical methods have not yet been developed especially in turbulent flows and at high heat fluxes. In general, the pressure drop in supercritical fluid flow consists of four components: pressure drop due to frictional resistance, due to local flow obstruction, due to acceleration of flow and due to gravity. The total pressure drop at supercritical pressures can be estimated using general correlations for pressure drop at subcritical pressures with correction factors for the effect of significant thermophysical properties variations and high heat fluxes. Only one paper was devoted to pressure drop in tight short bundles cooled with water at supercritical pressures. Therefore, more experimental work is needed to estimate the total pressure drop in various bundle designs with the objective of providing reliable information for designing supercritical water nuclear reactors.     

Dynamic Strain Aging of Nickel-Base Alloys 800H and 690

The objective of the current investigation is to characterize the dynamic strain aging (DSA) behavior in alloys 800H and 690. Constant extension rate tests were conducted at strain rates in the range of 10^?4 s^?1 to 10^?7?s^?1and temperatures between 295?K and 673?K (22?°C and 400?°C), in an argon atmosphere. Maps for the occurrence of serrated flow as a function of strain rate and temperature were built for both alloys. The enthalpy of serrated flow appearance of alloy 800H was found to be 1.07?±?0.30?eV.     

Optimization of excess system capability for increased evolvability

System evolvability is vital to the longevity of large-scale complex engineered systems. The need for evolvability in complex systems is a result of their long service lives, rapid advances to their integrated technologies, unforeseen operating conditions, and emerging system requirements. Building excess capability into complex systems can improve their ability to evolve while in service. However, excess capability increases initial build cost and operating cost, which is compounded across the service life of the system. Excess capability that is eventually used adds benefit by allowing for in-service evolution to meet emerging system requirements. Therefore, there is a trade-off between the cost of excess capability initially built into the system and the benefit that is added to the system by enabling future evolution. This paper introduces a process for optimizing the amount of excess capability in a complex system. This process results in a set of evolvable systems without excessive cost. We demonstrate how this process can be used to select the amount of excess capability that should be included in a military ground vehicle.     

The benefits of planar circular mouths on suction feeding performance

Suction feeding is the most common form of prey capture across aquatic feeding vertebrates and many adaptations that enhance efficiency and performance are expected. Many suction feeders have mechanisms that allow the mouth to form a planar and near-circular opening that is believed to have beneficial hydrodynamic effects. We explore the effects of the flattened and circular mouth opening through computational fluid dynamics simulations that allow comparisons with other mouth profiles. Compared to mouths with lateral notches, we find that the planar mouth opening results in higher flow rates into the mouth and a region of highest flow that is positioned at the centre of the mouth aperture. Planar mouths provide not only for better total fluid flow rates through the mouth but also through the centre of the mouth near where suction feeders position their prey. Circular mouths are shown to provide the quickest capture times for spherical and elliptical prey because they expose the prey item to a large region of high flow. Planar and circular mouths result in higher flow velocities with peak flow located at the centre of the mouth opening and they maximize the capacity of the suction feeders to exert hydrodynamic forces on the prey.     

Low temperature cure of epoxy thermosets attaining high Tg using a uniform microwave field

It is demonstrated for the first time that an epoxy thermoset resin can be cured at temperatures well below its T_g∞. This study compared the use of a uniform variable frequency microwave (VFM) field to standard oven curing at temperatures above and below T_g∞. Using T_g, tan δ, modulus, and FTIR measurements, it is shown that the reaction of BFDGE with MDA to attain a product with T_g∞ of 133 °C is achieved by VFM at temperatures from 100 to 140 °C; in contrast, the thermal cure normally requires 170 °C to attain the same T_g∞ and the same extent of cure. By following the pregel cure reaction with ¹³C‐NMR spectroscopy, it was determined that the lower cure temperatures of VFM cure predominately lead to chain extension and smaller amounts of crosslinking compared to the thermal cure. To explain these results, it is suggested that, after gelation, with VFM cure there is higher mobility from dipole rotations that continues the cure to completion without vitrification. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 44222.