Synergistic decrease of clonal proliferation, induction of differentiation, and apoptosis of acute promyelocytic leukemia cells after combined treatment with novel 20-epi vitamin D3 analogs and 9-cis retinoic acid

Patients with acute promyelocytic leukemia (APL) usually relapse after all-trans retinoic acid (RA) treatment because this therapy fails to eradicate the malignant clone. Our data showed that KH 1060 and other 20-epi vitamin D3 analogs alone were potent inhibitors of clonal growth of NB4 cells, an APL cell line (ED50, approximately 5 x 10(-11) M). The combination of KH 1060 and 9-cis-RA synergistically and irreversibly enhanced this effect. Neither KH 1060 nor 9-cis-RA (10(-6) M, 3 d) were strong inducers of differentiation of NB4 cells. However, 98% of the cells underwent differentiation to a mature phenotype with features of both granulocytes and monocytes after exposure to a combination of both compounds. Apoptosis only increased after incubation of NB4 cells with 9-cis-RA alone (28%) or with a combination of 9-cis-RA plus KH1060 (32%). Immunohistochemistry showed that the bcl-2 protein decreased from nearly 100% of the wild-type NB4 cells to 2% after incubation with a combination of KH 1060 and 9-cis-RA, and the bax protein increased from 50% of wild-type NB4 cells to 92% after culture with both analogs (5 x 10(-7) M, 3 d). Western blot analysis paralleled these results. Studies of APL cells from one untreated individual paralleled our results with NB4 cells. Taken together, the data demonstrated that nearly all of the NB4 cells can be irreversibly induced to differentiate terminally when exposed to the combination of KH 1060 and 9-cis-RA.     

The effect of a two-week anti-orthostatic hypokinesia on the cardiac function and on the latter''s regulation in monkeys

Medical practice guidelines are being developed rapidly and advocated as a crucial component in transforming and improving the delivery of health care services. However, little is known about the ability of hospitals to institute guidelines outside of experimental settings. This article approaches guideline implementation from the viewpoint of process innovation assimilation and presents specific recommendations for organizational tactics leading to successful implementation of practice guidelines.     

ZH150重油钢包烘烤装置的研制与应用

ＺＨ１５０重油钢包烘烤装置具有点火方便，雾化好，燃烧稳定，燃烧效率高，火焰刚性好，调节性能好等优点，适合于钢包的烘烤，也可应用在基他工业炉窑中，该装置用蒸汽或压缩空气作为雾化介质，能燃用柴油，原油，重油，渣油等液体燃料，介绍了该装置的基本结构，工作原理，工作特点及设计对策，以及在生产中的应用情况。     

一种新的坡位定量描述方法及其与滑坡发生的关系

滑坡发生与地形因子的关系是区域滑坡静态规律研究的重点,但以往的研究多注重考虑其与反映几何形态的基本地形属性因子(几何形态属性因子)之间的关系,如坡度和坡向等。首先提出一种新的基于子流域内相对位置定量描述坡位(相对位置因子)的方法,并以温州地区瓯江流域1990~2001年发生的滑坡为研究对象,使用证据权法对比滑坡发生与两种不同类型地形因子之间的关系。结果表明,相对位置因子与滑坡发生的相关性大于滑坡危险性评价经常使用的几何形态属性因子;并且滑坡大多发生在定量坡位值较低的区域,这些地区处在下游,遭受流水侵蚀的强度更大、频率更高。     

BP网络模型对人脸朝向的识别

研究BP神经网络模型,通过计算机模拟人脑建立神经元网络,使用一部分人脸朝向信息作为训练的实例集,训练稳健后,可以推广应用判断其他人脸的朝向信息,以实现计算机自动识别人脸朝向。先对图片进行归一化等预处理,再应用主成分分析提取特征信息,每幅提取出的特征信息都是8个数据的列向量,构建一个8个输入、17个隐含、3个输出的三层BP网络模型。将训练实例集的特征向量代入训练,调整参数后保证其性能和收敛速度。最后通过大量实验验证,计算机识别的误判率仅为6.7%,模型可靠。     

庐山风景区地质灾害成因与防御浅析

庐山风景区在江西省地质灾害区划中属于地质灾害易发区(修水北部——庐山崩滑流易发区),同时也是江西省地质灾害重点防范区.近些年,因人类工程活动对地质环境作用的加剧,加上地质环境条件在空间上的差异和气象条件在时空方面的变化,导致各类地质灾害不断发生.通过对庐山风景区地质灾害特点和成因分析,提出了防御工作思路与对策,以期为政府部门构建和完善地质灾害预警应急体系提供借鉴.     

Mechanically driven syntheses of carbides and silcides

Most metal carbides or slicides may be synthesized at room temperature, by ball milling mixtures of elemental powders for some tens of hours with a vibratory mill. Both stable and metastable compounds containing a high density of defects can be obtained. In general, phases stable at low temperatures are synthesized. This observation allows us to confirm recent estimations for the maximum temperature (600 K) attained in these powders during mechanical alloying. Exceptions are found for some MSi₂ suicides with M = titanium, iron or molybdenum for which both low- and high-temperature phases are formed.