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981.
RA Silva G Morales E Eulert A Montenegro R Yba?ez 《Canadian Metallurgical Quarterly》1998,76(1-2):153-157
Some new commercial methods for the extraction of viral RNA have been introduced recently. In addition to the study published previously (Verhofstede, C., Reniers, S., Van Wanzeele. F., Plum J., 1996. AIDS 8, 1421-1427), seven different methods (four newly developed and three reference methods) for extraction of HIV-1 RNA from plasma have been evaluated. The RNA preparation method that gave the best results (acceptable reproducibility, highest sensitivity, reasonable price, fast and easy to perform), was the QIAamp Viral RNA kit from QIAgen. The High Pure Viral RNA Kit (Boehringer Mannheim) as well as the non-commercialised extraction kits were also very sensitive. The non-commercial tests seem less suitable for routine use and for the processing of large number of samples. Two methods, RNA Insta-Pure LS (Eurogentec) and PANext RNA extraction kit 1 (NTL, PANsystems GmbH) are not adapted for HIV plasma extraction. The single step methods using glass fibre or silica column are rapid (from 60 to 75 min depending on the number of wash steps) and although the price is high they are cheaper than the Boom extraction methods: High Pure Viral RNA Kit (Boehringer Mannheim) ($3.3/sample), QIAamp Viral RNA Kit (Qiagen) ($3.6/sample), Boom extraction ($5/sample). The Qiagen kit is the only kit that combines sensitivity with reproducibility, it is commercialised, rapid and affordable in price and can be automated. For most of the methods evaluated the inter-test variability was acceptable (mean variation coefficient between duplicate extractions varied between 26.4 and 48.6%). 相似文献
982.
983.
984.
R. Ushatov H. Power J. J. Rê go Silva 《Engineering Analysis with Boundary Elements》1994,13(4):371-381
In this work, uniform bicubic B-spline functions are used to represent the surface geometry and interpolation functions in the formulation of boundary-element method (BEM) for three-dimensional problems. This is done as a natural generalization of cubic B-spline curves, introduced by Cabral et al, for two-dimensional problems. Three-dimensional scalar problems, with particular applications to Laplace and Helmholtz equations, are considered. 相似文献
985.
AA de Paola CE Balb?o O Silva Netto A Mendon?a H Villacorta AC Vattimo IA Souza N Guiguer Júnior OP Portugal EE Martinez Filho 《Canadian Metallurgical Quarterly》1993,60(2):65-70
The author suggests a working classification of dermopertorators from analysis of his own designs and devices described in the literature for forming netlike skin grafts in the treatment of patients with large tissue defects. It is shown that the well-known dermoperforators require essential modernization. The most promising model is a device with plane knives whose simple technology allows their production to be organized in Soviet industry. 相似文献
986.
A protocol was designed for the rapid and efficient construction of cosmid libraries from cell-associated viral genomes available in very low quantities. Purification of viral DNA from cellular DNA was unnecessary. The vast excess of cellular DNA compensated for the limited amount of available viral DNA, enabling titration of the restriction endonuclease partial digest. A cosmid library of the turkey herpesvirus DNA genome was constructed from 1.5 micrograms of cellular DNA containing approximately 6 nanograms of viral DNA. 相似文献
987.
988.
A galactomannoglucan (GMG) with an estimated weight–average molar mass of 415,000 g/mol was obtained from an aqueous extract of the mesocarp of fruits of Syagrus oleracea (Mart.) Becc. by fractionation by Sephacryl S-300 HR and Sephadex G-25. Chemical and spectroscopic studies indicated that GMG has a chain of (1 → 4)-linked β-d-mannopyranosyl residues attached to an initial chain of (1 → 3)-linked β-d-galactopyranosyl residues and a terminal chain of (1 → 4)-linked α-d-glucopyranosyl residues which comprised galactose, mannose and glucose in the molar ratio of 30:33:37. Results of the present study indicated that the polysaccharide GMG of S. oleracea significantly inhibited gastric lesions induced by ethanol, showing a gastroprotective property. 相似文献
989.
Danielle Marques Vilela Gilberto Vinícius de M. Pereira Cristina Ferreira Silva Luís Roberto Batista Rosane Freitas Schwan 《Food microbiology》2010
This work was aimed at isolating and identifying the microbiota present during the semi-dry method of coffee processing using polyphasic methods and to evaluate microbial diversity with PCR-DGGE. Samples of Coffea arabica L. were collected during different processing stages in southern Minas Gerais, Brazil. The bacterial and fungal isolates were phenotypically characterised and grouped according to the ARDRA technique, in which the 16–23S and ITS1-5.8S regions of the rDNA were sequenced for species identification. The bacterial counts varied from 3.7 to 7 log CFU g−1. The yeast counts ranged from 3.4 to 6.9 log CFU g−1, and the filamentous fungal population varied from 2 to 3.7 log CFU g−1. Bacillus subtilis, Escherichia coli, Enterobacter agglomerans, Bacillus cereus and Klebsiella pneumoniae were the predominant bacteria detected during the processing of the coffee, and Pichia anomala, Torulaspora delbrueckii and Rhodotorula mucilaginosa were the dominant yeasts. All of the yeast and bacterial species detected by PCR-DGGE were isolated using culture-dependent methods, with the exception of one uncultivable bacterial species. Aspergillus was the most common genus among the filamentous fungal isolates. The use of polyphasic methods allowed a better characterization of the microbiota that is naturally present in semi-dry processed coffee. 相似文献
990.
Patrícia da Silva Malheiros Daniel Joner Daroit Nádya Pesce da Silveira Adriano Brandelli 《Food microbiology》2010
Commercial nisin was encapsulated in nanovesicles (mean diameter 140 nm) prepared from partially purified soy lecithin. Nisin-loaded liposomes and unencapsulated (free) nisin were initially tested in BHI medium and skim milk inoculated with Listeria monocytogenes and incubated for 48 h at 30 °C. At such abuse temperature conditions, free nisin showed better inhibitory than the liposomal counterparts. Subsequently, the effect of encapsulated or free nisin was evaluated in combination with refrigeration (7 ± 1 °C) in both whole (3.25% fat) and skim (0% fat) milk for up to 14 day. A decrease of 3–4 log cycles in L. monocytogenes counts was observed for free and encapsulated nisin at 0.5 mg/ml concentration. Liposome encapsulation of antimicrobial peptides may be important to overcome stability issues and interaction with food components. The utilization of nanovesicle-encapsulated nisin in combination with low temperatures appeared to be effective to control L. monocytogenes in milk, emphasizing the importance of hurdle technology to assure food safety. 相似文献