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41.
Optimization of bioprocess for production of copper-enriched biomass of industrially important microorganism Saccharomyces cerevisiae 总被引:1,自引:0,他引:1
Mrvcić J Stanzer D Stehlik-Tomas V Skevin D Grba S 《Journal of Bioscience and Bioengineering》2007,103(4):331-337
The production of Saccharomyces cerevisiae cells enriched with copper and the effects of adding copper ions to different media on yeast cell growth and ethanol production were studied. In the media Cu(2+) concentrations of up to 0.094 mM had no effect on alcoholic fermentation, whereas higher Cu(2+) concentrations markedly decreased yeast cell growth rate and ethanol production. Under static conditions, the maximum amounts of copper uptake (i.e., 1.16 mg/g, 1.2 mg/g and 0.81 mg/g dry matter yeast biomass for glucose, sucrose and molasses media, respectively) were obtained after 8 h of fermentation, whereas under dynamic conditions smaller amounts of copper uptake (i.e., 0.98 mg/g, 1.02 mg/g and 0.7 mg/g dry matter yeast biomass for glucose, sucrose and molasses media, respectively) were obtained after 6 h of fermentation. 相似文献
42.
Željka Jovanović Jasmina StojkovskaBojana Obradović Vesna Mišković-Stanković 《Materials Chemistry and Physics》2012
An innovative method was developed for production of alginate hydrogel microbeads incorporated with silver nanoparticles (AgNPs) based on electrochemical synthesis followed by electrostatic extrusion. AgNPs were synthesized galvanostatically at different values of AgNO3 concentration in the initial solution (0.5–3.9 mM), current density (5–50 mA cm−2), and implementation time (0.5–10 min). Increase in all of these parameters increased the concentration of AgNPs in alginate solution and was confirmed by TEM analysis and UV–vis spectroscopy. Cyclic voltammetry studies and Fourier transform infrared spectroscopy proved the alginate to be a good capping agent for the electrochemical synthesis of silver nanoparticles, due to coordination bonding between hydroxyl and ether groups, as well as ring oxygen atoms in uronic acid residues of alginate molecules, and Ag nanoparticles. Ag/alginate colloid solution was used for production of uniform hydrogel microbeads (with diameter of 487.75 ± 16.5 μm) by electrostatic extrusion technique. UV–vis spectroscopy confirmed retention and entrapment of AgNPs in microbeads during the production process. Alginate microbeads incorporated with AgNPs are attractive as biocompatible carriers and/or efficient donors of AgNPs as active components especially for potential biomedical applications, which was demonstrated by the antibacterial activity against Staphylococcus aureus. 相似文献
43.
Obradovic B Stojkovska J Jovanovic Z Miskovic-Stankovic V 《Journal of materials science. Materials in medicine》2012,23(1):99-107
Alginate colloid solution containing electrochemically synthesized silver nanoparticles (AgNPs) was investigated regarding
the nanoparticle stabilization and possibilities for production of alginate based nanocomposite hydrogels in different forms.
AgNPs were shown to continue to grow in alginate solutions for additional 3 days after the synthesis by aggregative mechanism
and Ostwald ripening. Thereafter, the colloid solution remains stable for 30 days and could be used alone or in mixtures with
aqueous solutions of poly(vinyl alcohol) (PVA) and poly(N-vinyl-2-pyrrolidone) (PVP) while preserving AgNPs as verified by UV–Vis spectroscopy studies. We have optimized techniques
for production of Ag/alginate microbeads and Ag/alginate/PVA beads, which were shown to efficiently release AgNPs decreasing
the Escherichia coli concentration in suspensions for 99.9% over 24 h. Furthermore, Ag/hydrogel discs based on alginate, PVA and PVP were produced
by freezing-thawing technique allowing adjustments of hydrogel composition and mechanical properties as demonstrated in compression
studies performed in a biomimetic bioreactor. 相似文献
44.
Transferable Antibiotic Resistances in Marketed Edible Grasshoppers (Locusta migratoria migratorioides) 下载免费PDF全文
Andrea Osimani Cristiana Garofalo Lucia Aquilanti Vesna Milanović Federica Cardinali Manuela Taccari Marina Pasquini Stefano Tavoletti Francesca Clementi 《Journal of food science》2017,82(5):1184-1192
Grasshoppers are the most commonly eaten insects by humans worldwide, as they are rich in proteins and micronutrients. This study aimed to assess the occurrence of transferable antibiotic resistance genes in commercialized edible grasshoppers. To this end, the prevalence of 12 selected genes [aac(6’)‐Ie aph(2″)‐Ia, blaZ, erm(A), erm(B), erm(C), mecA, tet(M), tet(O), tet(S), tet(K), vanA, vanB] coding for resistance to antibiotics conventionally used in clinical practice was determined. The majority of samples were positive for tet(M) (70.0%), tet(K) (83.3%) and blaZ (83.3%). A low percentage of samples were positive for erm(B) (16.7%), erm(C) (26.7%), and aac(6’)‐Ie aph(2″)‐Ia (13.3%), whereas no samples were positive for erm(A), vanA, vanB, tet(O), and mecA. Cluster analysis identified 4 main clusters, allowing a separation of samples on the basis of their country of origin. 相似文献
45.
Investigation of technological approaches for reduction of methanol formation in plum wines 下载免费PDF全文
Uroš Miljić Vladimir Puškaš Vesna Vučurović 《Journal of the Institute of Brewing》2016,122(4):635-643
Methanol is natural ingredient of alcoholic beverages and soft drinks; however, the products of its metabolic transformations (formaldehyde and formic acid) are toxic to humans. The aim of this research was to assess the application of different physico‐chemical treatments of pomace in order to find the most efficient method for reducing the methanol during fermentation with the least effect on the sensory properties of the wine. The following procedures were studied: addition of tannins in pomace, addition of phenolic acids, addition of d ‐galacturonic and pectic acid, use of bentonite and zeolite, heat treatment of pomace, thermosonication and treatment of pomace with microwaves. Fruit wine used in this study was produced from plums (Prunus domestica L.). Applied treatments showed variable efficiency in reduction of methanol formation in plum wine. It may be noted that the procedures that involved some form of thermal treatment were characterized by a significant decrease in the production of methanol (up to 60–70%) but mostly tended to have a negative impact on the sensory properties of the produced wines. However, exposure of pomace to microwaves for a short time, owing to the contribution of mechanisms of non‐thermal nature (kinetic and chemical), allowed for a significant reduction in methanol formation with a negligible impact on the sensory properties. The decrease in methanol formation during fermentation using tannins, pectic acid, bentonite and zeolite was poor, with only a reduction of up to 15%. Obtained results are significant to the fruit wine and spirit industry, considering the common problem related to an increased content of methanol in these beverages. Copyright © 2016 The Institute of Brewing & Distilling 相似文献
46.
Sanja Eraković Djordje Veljović Papa N. Diouf Tatjana Stevanović Miodrag Mitrić Djordje Janaćković Ivana Z. Matić Zorica D. Juranić Vesna Mišković-Stanković 《Progress in Organic Coatings》2012
Composite coatings based on lignin, obtained by electrophoretic deposition (EPD) on titanium, were investigated. The aim of this work was to produce hydroxyapatite/lignin (HAP/Lig) coatings on titanium and to investigate the effect of the lignin concentration on microstructure, morphology, phase composition, thermal behavior and cytotoxicity of the HAP/Lig coatings. An organosolv lignin was used for the production of the composite coatings studied in this research. The properties of HAP/Lig coatings were characterized using X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FT-IR) and X-ray photoelectron spectroscopy (XPS), as well as the MTT test of cytotoxicity. The results showed that higher lignin concentrations protected the HAP lattice during sintering, thereby improving the stability of the HAP/Lig coatings. The cell survival of peripheral blood mononuclear cells (PBMC) after proliferation indicates that the HAP/Lig coating with 1 wt% Lig electrodeposited on titanium was non-toxic with significant promise as a potential bone-repair material. 相似文献
47.
Tumbas VT Canadanović-Brunet JM Cetojević-Simin DD Cetković GS Ethilas SM Gille L 《Journal of the science of food and agriculture》2012,92(6):1273-1281
BACKGROUND: The commercial development of plants as sources of antioxidants that can be used to enhance the properties of foods, for nutritional purposes and preservation as well as for prevention of oxidation‐related diseases, is currently of major interest. Rosehip (Rosa canina L.) is a rich source of vitamin C and polyphenols. RESULTS: Phytochemicals in rosehip tea were separated into three fractions: Fr1 (vitamin C, 39.17 mg kg?1), Fr2 (flavonoids, 451.05 µg kg?1) and Fr3 (phenolic acids, 504.69 µg kg?1). Quercetin and ellagic acid were the most abundant polyphenolic compounds. Rosehip fractions, primarily rosehip flavonoids (EC50 = 49 mg L?1), showed high antioxidant activity towards 2,2‐diphenyl‐1‐picrylhydrazyl radicals (DPPH?). Cell growth effects of rosehip fractions were assessed in HeLa, MCF7 and HT‐29 cell lines, with the lowest IC50 values being determined for rosehip flavonoids, (80.63, 248.03 and 363.95 mg L?1 respectively). However, the vitamin C fraction did not inhibit the growth of tested tumour cells. CONCLUSION: The results of this study confirm that vitamin C and flavonoids are responsible for the antioxidant activity of rosehip tea, while only polyphenols contribute to its antiproliferative activity. Copyright © 2011 Society of Chemical Industry 相似文献
48.
Jelena Vulić Jasna Čanadanović-Brunet Gordana Ćetković Vesna Tumbas Sonja Djilas Dragana Četojević-Simin Vladimir Čanadanović 《Journal of Functional Foods》2012,4(3):670-678
Beetroot (Beta vulgaris) pomace, processing by-product from food industry, was investigated in this work as a starting raw material. The contents of phenolics (1.87–11.98 mg GAE/g of dry weight) and betalains (0.75–3.75 mg betalains/g of dry weight) in the extracts were determinated spectrophotometrically. Some individual phenolic compounds, including ferulic, vanillic, p-hydroxybenzoic, caffeic and protocatehuic acids, were identified and quantified by HPLC. The antioxidative activity of beetroot extracts was tested using DPPH, hydroxyl and superoxide anion radicals by ESR spectroscopy. (0.05–0.108 mg/ml), (0.183–0.333 mg/ml), and (0.198–1.000 mg/ml) were calculated. The regression analysis produced moderate to high correlation coefficients between the scavenging activities and phenolics, and some individual phenolic compounds which indicated that beetroot pomace may be used as functional food ingredient. Cell growth effects were determined in MCF7 and MRC-5 cell lines, using SRB assay. IC50 values were in the range of 362.478–503.525 and 383–587.880 μg/ml in MRC-5 and MCF7, respectively. 相似文献
49.
Inferring the origin of rare fruit distillates from compositional data using multivariate statistical analyses and the identification of new flavour constituents 下载免费PDF全文
50.
Vesna Župunski Mirjana Vasić Jelka Šuštar Vozlič Marko Maras Aleksandra Savić Gordana Petrović Dalibor Živanov 《Food Analytical Methods》2018,11(4):1034-1040
Trypsin inhibitors could limit utilization of legumes in human nutrition, but they could also have beneficial health effects. The objective of this study was to measure trypsin inhibitor activity (TIA) of different legumes using microtiter plate method and to identify factors that contribute to uncertainty of TIA measurement. TIA measurements were performed on seeds of faba bean, pea, common vetch, soybean, and common bean cultivars. The significant effect of legume crop on TIA measurement uncertainty was confirmed with P = 0.045. Certain sources of measurement uncertainty were related with the content of trypsin inhibitors (Tis) in legume seeds. In respect to that, significant effect of level of sample dilution (P ? 0.001) was confirmed. Significant influence of the repeated absorbance measurement of sample reaction mixture on uncertainty of TIA measurement was identified (P ? 0.001), and it took 60% of overall TIA measurement uncertainty for soybean cultivars. TIA of soybean cultivars exceeded 90 TUI/mg. Repeated absorbance measurement of positive control reaction mixture took 70% of TIA measurement uncertainty of cultivars with TIA lesser than 4.5 TUI/mg. Graduated cylinder used for preparation of the final sample solutions took the range from 45 to 90% of overall TIA measurement uncertainty of the cultivars whose TIA were in the middle of previously mentioned. The uncertainty of TIA measurement of legume crops was not studied before; thus, this study pointed out that acquiring insight into factors contributing to uncertainty of TIA measurement could give directions for improvement of TIA testing if microtiter plate method is used. 相似文献