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81.
High-Fat Diet Alters Serum Fatty Acid Profiles in Obesity Prone Rats: Implications for In Vitro Studies
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Liu Tzu-Wen Heden Timothy D. Matthew Morris E. Fritsche Kevin L. Vieira-Potter Victoria J. Thyfault John P. 《Lipids》2015,50(10):997-1008
High‐fat diets (HFD) are commonly used in rodents to induce obesity, increase serum fatty acids and induce lipotoxicity in various organs. Invitro studies commonly utilize individual free fatty acids (FFA) to study lipid exposure in an effort to model what is occurring in vivo; however, these approaches are not physiological as tissues are exposed to multiple fatty acids in vivo. Here we characterize circulating lipids in obesity‐prone rats fed an HFD in both fasted and fed states with the goal of developing physiologically relevant fatty acid mixtures for subsequent in vitro studies. Rats were fed an HFD (60 % kcal fat) or a control diet (10 % kcal fat) for 3 weeks; liver tissue and both portal and systemic blood were collected. Fatty acid profiles and absolute concentrations of triglycerides (TAG) and FFA in the serum and TAG, diacylglycerol (DAG) and phospholipids in the liver were measured. Surprisingly, both systemic and portal serum TAG were ~40 % lower in HFD‐fed compared to controls. Overall, compared to the control diet, HFD feeding consistently induced an increase in the proportion of circulating polyunsaturated fatty acids (PUFA) with a concomitant decline in monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA) in both serum TAG and FFA. The elevations of PUFA were mostly attributed to increases in n‐6 PUFA, linoleic acid and arachidonic acid. In conclusion, fatty acid mixtures enriched with linoleic and arachidonic acid in addition to SFA and MUFA should be utilized for in vitro studies attempting to model lipid exposures that occur during in vivo HFD conditions. 相似文献
82.
Victoria Derr 《CoDesign》2015,11(2):119-133
This article shares outcomes from a year-long participatory planning process – one that brought together children and youth engagement, city planning, campus planning, and a university environmental design course to explore child-friendly, dense and affordable housing for a city of approximately 100,000 residents. This process of engagement asked design students to include youth participation and child-friendly cities as an integrated component of sustainable cities. The article shows varying degrees of transformation, views of children as credible participants, and values gained from the project. Many undergraduates changed their thinking about the role of young people in design processes and outcomes. 相似文献
83.
Rubn Núez Chen Jin Marta Victoria Csar Domínguez Stephen Askins Rebeca Herrero Ignacio Antn Gabriel Sala 《Progress in Photovoltaics: Research and Applications》2016,24(9):1214-1228
Multi‐junction solar cells are widely used in high‐concentration photovoltaic systems (HCPV) attaining the highest efficiencies in photovoltaic energy generation. This technology is more dependent on the spectral variations of the impinging Direct Normal Irradiance (DNI) than conventional photovoltaics based on silicon solar cells and consequently demands a deeper knowledge of the solar resource characteristics. This article explores the capabilities of spectral indexes, namely, spectral matching ratios (SMR), to spectrally characterize the annual irradiation reaching a particular location on the Earth and to provide the necessary information for the spectral optimization of a MJ solar cell in that location as a starting point for CPV module spectral tuning. Additionally, the relationship between such indexes and the atmosphere parameters, such as the aerosol optical depth (AOD), precipitable water (PW), and air mass (AM), is discussed using radiative transfer models such as SMARTS to generate the spectrally resolved DNI. The network of ground‐based sun and sky‐scanning radiometers AErosol RObotic NETwork (AERONET) is exploited to obtain the atmosphere parameters for a selected bunch of 34 sites worldwide. Finally, the SMR indexes are obtained for every location, and a comparative analysis is carried out for four architectures of triple junction solar cells, covering both lattice match and metamorphic technologies. The differences found among cell technologies are much less significant than among locations. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
84.
Alvaro-Fuentes J Arrúe JL Gracia R López MV 《The Science of the total environment》2007,378(1-2):179-182
During decades, in semiarid rainfed Aragon, intensive soil tillage and low crop residue input have led to the loss of soil structure and soil degradation. Conservation tillage and cropping intensification can improve soil structure in these areas. The objective of this study was to determine the influence of three different tillage systems (traditional tillage, reduced tillage and no-tillage) under two cropping systems (fallow-barley rotation and barley monoculture) on soil aggregation dynamics during a cropping season. A decrease in tillage intensity resulted in a higher mean size of dry aggregates and a greater water aggregate stability in both cropping systems particularly under no-tillage. 相似文献
85.
The use of azo dyes in industrial activities generates a large volume of contaminated wastewater; these pollutants in water bodies affect aquatic biota and human health. A functional biocomposite sorbent material was synthesized using cross-linked chitosan with oxalic acid that forms a coating on alumina ceramic particles (AOCh). The removal of Reactive Red 195, a reactive azo dye, using a fixed-bed adsorption column filled with this material was tested. AOCh was physico-chemically characterized by Fourier transform infrared spectroscopy–total attenuated reflection (FTIR-ATR), scanning electron microscopy–energy dispersion spectrometry X-ray (SEM-EDS), X-ray diffraction (XDR), thermo-gravimetric analysis (TGA), and Z-potential. The dynamic adsorption performance was analyzed from experimental breakthrough curves obtained in fixed-bed columns by modifying different operating conditions (bed depth, volumetric flow rate, and dye inlet concentration). Equilibrium adsorption isotherms were determined under dynamic conditions and compared with batch results. The maximum adsorption capacity of the dynamic equilibrium isotherm obtained from the continuous assays was 331 mg/g; this value was the highest in comparison to other tested materials reported in the literature. Different dynamic adsorption models were applied to fit experimental data, including Thomas, Bohart–Admas, Yoon–Nelson, logistic general model, bed depth surface time (BDST), and modified dose response (Yan) models. A critical analysis of these equations was presented, showing the equivalences and the relationship among the coefficients. The Yan model achieved the highest level of agreement between the experimental and predicted values of the breakthrough curves. The use of this model enables scaling-up the industrial process for dye removal. The present work proposed a novel biosorbent material and contributes to the analysis of industrial dye removal under dynamic conditions. 相似文献
86.
M Victoria Avanza M Cristina Añón 《Journal of the science of food and agriculture》2007,87(4):616-623
The effect of thermal treatment of proteins from Amaranthus hypochondriacus was studied. Two protein isolates were obtained from the defatted flour by water extraction at a pH of 9 (A9 isolate) and 11 (A11 isolate), followed by isoelectric precipitation at a pH of 5. Effect of thermal treatment (70 and 90 °C, during 3, 5, 10, 15 and 30 min) on A9 and A11 dispersions were analyzed by differential scanning calorimetry (DSC), polyacrylamide gel electrophoresis, UV spectrophotometry, superficial hydrophobicity and solubility in water. Thermal treatment induced the aggregate formation of high molecular mass stabilized by disulfide and non‐covalent bond. Thermal treatment at 70 °C produced a 30% denaturation in both, while at 90 °C A9 was more denatured than A11 (75% and 55% of denaturation, respectively). An increase in thermal stability was also detected by DSC in A9 treated at 90 °C. The denaturation process was accompanied at short heating times by an increase in UV absorbance and changes in superficial hydrophobicity. A decrease in water solubility (35–50%, depending on time–temperature conditions) was also observed for the A9 isolates. The results suggest that the A9 isolates, enriched in a globulin protein fraction, are more sensitive to thermal treatment than isolates A11 enriched in glutelin protein fraction. The changes shown by both isolates, indeed, could affect their functional properties and could definitely limit their use in food products. Copyright © 2007 Society of Chemical Industry 相似文献
87.
Localization and speciation of chromium in subterranean clover using XRF, XANES, and EPR spectroscopy 总被引:2,自引:0,他引:2
Howe JA Loeppert RH DeRose VJ Hunter DB Bertsch PM 《Environmental science & technology》2003,37(18):4091-4097
Optimization of phytoremediation and assessment of potential health hazards from metals in the environment requires an understanding of absorption, localization, and transport of the target metal by plants. The objectives of this study were to localize Cr and determine the oxidation state and possible complexation mode of Cr in intact plant tissue by means of XANES, synchrotron XRF microprobe spectroscopy, and EPR spectroscopy. Subterranean clover (Trifolium brachycalycinum) was grown hydroponically with Cr(VI) (0.04-2.0 mmol L(-1)) and compared with plants grown without Cr and with inorganic Cr(III) and various Cr(III)-organic sources. The uptake, translocation, and form of Cr in the plant were dependent on the form and concentration of supplied Cr. Chromium was found predominately in the +3 oxidation state, regardless of the Cr source supplied to the plant, though at high Cr(VI) treatment concentrations, Cr(VI) and Cr(V) were also observed. At low Cr(VI) concentrations, the plant effectively reduced the toxic Cr(VI) to less toxic Cr(III), which was observed both as a Cr(III) hydroxide phase at the roots and as a Cr(III)-organic complex in the roots and shoots. At low Cr(VI) treatment concentrations, Cr in the leaves was observed predominately around the leaf margins, while at higher concentrations Cr was accumulated at leaf veins. 相似文献
88.
Navas J Ortiz S López P López V Martínez-Suárez JV 《Journal of food protection》2007,70(12):2851-2854
This study aimed to evaluate the effect of different enrichment procedures on the detection of Listeria monocytogenes in food, by a comparison of subculture onto chromogenic agar with real-time PCR. Two different culture media, the primary and secondary enrichment broths of the U.S. Food Safety and Inspection Service (FSIS) method used for PCR detection of L. monocytogenes, were compared for the primary enrichment of retail ground chicken samples. L. monocytogenes was detected after the completion of each enrichment procedure in 63% (complete FSIS procedure) and 60% (plain FSIS secondary enrichment broth incubated for 48 h) of the samples by both culture and PCR, whereas a combination of the results for the two enrichment procedures revealed 86% of the samples to be positive. Most of the samples analyzed contained a mixture of lineage I and II strains, and their ratio varied for each enrichment procedure. This mixture could have a significant effect on the result of detection of L. monocytogenes for each individual sample, explaining the increase in positive samples when the results of the two enrichment procedures were combined. The use of different isolation procedures can affect the specific samples identified as positive and the specific strains isolated. 相似文献
89.
Ultra‐high‐pressure homogenization (UHPH) system for producing high‐quality vegetable‐based beverages: physicochemical,microbiological, nutritional and toxicological characteristics
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90.
Trends in Hydrological Parameters of a Southern Brazilian Watershed and its Relation to Human Induced Changes 总被引:4,自引:1,他引:4
Moraes Jorge M. Pellegrino Giampaolo Q. Ballester Maria V. Martinelli Luiz A. Victoria Reynaldo L. Krusche Alex V. 《Water Resources Management》1998,12(4):295-311
The Piracicaba river basin is a subtropical watershed located in the southeastern region of Brazil. With an area of 12 400 km2, the basin is a typical example of new landscape resulting from development in tropical and sub-tropical regions: establishment of intensive industrial and agricultural processes were followed by significant population growth and water management. This scenario has led to significant increase in water demand and decrease in water quality. The main objective of this study is the detection of changes in the patterns of flow and precipitation in the basin, and its possible relation to man-induced changes. Statistical analyses were performed on records of precipitation, evapotranspiration and streamflow, from 1947 to 1991. Precipitation and evapotranspiration totals showed significant increasing trends for the entire basin. From eight streamflow gauge stations, half showed significant decreasing trend. The most probable cause of such trends is the export of water from the basin to the metropolitan region of São Paulo city. 相似文献