Design,optimization and evaluation of poly-ɛ-caprolactone (PCL) based polymeric nanoparticles for oral delivery of lopinavir

Lopinavir (LPV)-loaded poly-ε-caprolactone (PCL) nanoparticles (NPs) were prepared by emulsion solvent evaporation technique. Effects of various critical factors in preparation of loaded NPs were investigated. Box–Behnken design (BBD) was employed to optimize particle size and entrapment efficiency (EE) of loaded NPs. Optimized LPV NPs exhibited nanometeric size (195.3?nm) with high EE (93.9%). In vitro drug release study showed bi-phasic sustained release behavior of LPV from NPs. Pharmacokinetic study results in male Wistar rats indicated an increase in oral bioavailability of LPV by 4-folds after incorporation into PCL NPs. From tissue distribution studies, significant accumulation of loaded NPs in tissues like liver and spleen indicated possible involvement of lymphatic route in absorption of NPs. Mechanistic studies using rat everted gut sac model revealed endocytosis as a principal mechanism of NPs uptake. In vitro rat microsomal metabolism studies demonstrated noticeable advantage of LPV NPs by affording metabolic protection to LPV. These studies indicate usefulness of PCL NPs in enhancing oral bioavailability and improving pharmacokinetic profile of LPV.     

A study on utilization of ground source energy for space heating using a nanofluid as a heat carrier

Ground source heat pump (GSHP) systems are well established as an energy-efficient space conditioning device. However, for better utilization of the ground source, improvement in GSHP performance is desirable, which limits the small temperature difference between the ground and the circulating fluid. In this study, efforts have been made to investigate the performance of a ground heat exchanger (GHX) with a nanofluid as a heat carrier. Mathematical modeling is performed for the closed-loop vertical U-tube GHX with six different (Al₂O₃, CuO, graphite, multiwalled carbon nanotube, graphene, and Cu) water-based nanofluids. The effect of different operating parameters on GHX length, fluid temperature, and pressure drop with nanofluids is determined. On the basis of the analytical results, it is found that the graphite particle-based nanofluid plays a prominent role to enhance the performance of the GHX as compared with other nanoparticles. The maximum enhancement in the increase in outlet fluid temperature and reduction in pipe length with graphite particle-based nanofluid are 68.3% and 63.3%, respectively, for an increase in temperature difference from 7°C to 15°C between the atmosphere and the ground. Also, with the graphite particle-based nanofluid and the increase in pipe diameter from 20 to 50 mm, the fluid outlet temperature increases up to 11.2%, and the requirement in GHX length reduces up to 55%.     

Synthesis,structural and photoluminescence properties of SiOx nanospheres prepared by evaporation of silicon monoxide

We report synthesis of amorphous silicon oxide nano-spheres in bulk quantity by using a simple non-catalytic process based on thermal evaporation of silicon monoxide. Structural characteristics of the nano-spheres were investigated using high resolution scanning and transmission electron microscopes equipped with energy dispersive X-ray spectroscopy (EDAX). Selected area electron diffraction and EDAX analyses revealed that nano-spheres were amorphous and comprised of silicon oxide only with Si and O in an atomic ratio of ~ 1:2. Photoluminescence measurements showed that the SiO_x nano-spheres had strong emission band in the blue region. Possible growth mechanism based on vapor-solid model has been discussed. The SiO vapors generated at high temperature react with O₂ to form SiO_x vapors which subsequently condense on the substrates (placed in the appropriate temperature zone) to form SiO_x nano-spheres.     

Impermeable graphenic encasement of bacteria

Transmission electron microscopy (TEM) of hygroscopic, permeable, and electron-absorbing biological cells has been an important challenge due to the volumetric shrinkage, electrostatic charging, and structural degradation of cells under high vacuum and fixed electron beam.(1-3) Here we show that bacterial cells can be encased within a graphenic chamber to preserve their dimensional and topological characteristics under high vacuum (10(-5) Torr) and beam current (150 A/cm(2)). The strongly repelling π clouds in the interstitial sites of graphene's lattice(4) reduces the graphene-encased-cell's permeability(5) from 7.6-20 nm/s to 0 nm/s. The C-C bond flexibility(5,6) enables conformal encasement of cells. Additionally, graphene's high Young's modulus(6,7) retains cell's structural integrity under TEM conditions, while its high electrical(8) and thermal conductivity(9) significantly abates electrostatic charging. We envision that the graphenic encasement approach will facilitate real-time TEM imaging of fluidic samples and potentially biochemical activity.     

Investigation of mechanical properties of friction-welded AISI 304 with AISI 1021 dissimilar steels

Austenitic stainless steel and low alloy steels are extensively used in various automotive, aerospace, nuclear, chemical, and other general purpose applications. Joining of dissimilar metals is one of the challenging tasks and most essential need of the present-day industry. It has been observed that a wide range of dissimilar materials can be easily integrated by friction welding. The objectives of the present investigation were obtaining weldments between austenitic stainless steel (AISI 304) with low alloy steel (AISI 1021) and optimizing the friction welding parameters in order to establish the weld quality. In the present study, an experimental setup was designed in order to achieve friction welding of plastically deformed austenitic stainless steel and low alloy steel. AISI 304 and AISI 1021 steels were welded by friction welding using five different axial pressures at 1,430 rpm. The joining performances of friction-welded dissimilar joints were studied, and influences of these process parameters on the mechanical properties of the friction-welded joints were estimated. The joint strength was determined with tensile testing, and the fracture behavior was examined by scanning electron microscopy (SEM) and was supported and backed by energy dispersive spectroscopy (EDS) analysis. Furthermore, the proposed joints were tested for impact strength, and the microhardness across the joint was also evaluated.     

Technological and policy innovations toward cleaner development

Clean Technologies and Environmental Policy -     

Study of phases formed during production of copper indium diselenide by reacting copper, indium and selenium layers

CuInSe₂ films were grown by reacting stacked layers of Cu, In and Se in an atmosphere of Se vapor. Incremental growth of the various phases was followed at different temperatures until a single phase CuInSe₂ film was formed. Conventional X-ray diffraction was used in identifying the different phases formed. Along with the knowledge of different phases formed at increasing reaction temperatures, it was concluded that CuInSe₂ is formed at temperatures as low as 235°C, although a single phase film is obtained only at higher temperatures (≈350°C).     

Effect of heat treatment on fatigue crack growth in Ti---6Al---3.5Mo---1.9Zr---0.23Si alloy

The effect of heat treatment on fatigue crack growth in Ti---6.5Al---3.5Mo---1.9Zr---0.23Si has been studied. Recrystallization annealing (960 °C/2 h) followed by a water quench from within the +β field (875 °C) leads to a significant improvement in the fatigue crack growth resistance as compared to a conventional heat treatment, owing primarily to a transition which occurs at ΔK=20 MPa √m. The improvement in fatigue crack growth resistance can be attributed to the presence of twinning as the primary mode of deformation in the metastable β-phase.     

Damage Detection and Damage Detectability— Analysis and Experiments

A technique to identify structural damage in real time using limited instrumentation is presented. Contrast maximization is used to find the excitation forces that create maximum differences in the response of the damaged structure and the analytical response of the undamaged structure. The optimal excitations for the damage structure are then matched against a database of optimal excitations to locate the damage. To increase the reliability of the approach under modeling and measurement errors, the contrast maximization approach is combined with an approach based on changes in frequency signature. The detectability of any particular damage with the proposed technique depends on the ratio of the magnitude of damage and the magnitude of errors in the measurements, as well as on how much the damage influences the measurements. A damage detectability prediction measure, that incorporates these effects, is developed. The technique is first tested numerically on a 36 degree-of-freedom space truss. To simulate experimental conditions, an extensive study is carried out in the presence of noise. A similar truss is then built and the finite-element method (FEM) model of the structure is corrected using experimental data. The technique is applied to locate the damage in several members. The experimental results indicate that this technique can robustly identify the damaged member with limited measurements and real-time computation. The effectiveness of the damage detection measure is also demonstrated.