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61.
62.
JR Nofer M Walter B Kehrel S Wierwille M Tepel U Seedorf G Assmann 《Canadian Metallurgical Quarterly》1998,18(6):861-869
We demonstrate that physiological concentrations of HDL3 inhibit the thrombin-induced platelet fibrinogen binding and aggregation in a time- and concentration-dependent fashion. The underlying mechanism includes HDL3-mediated inhibition of phosphatidylinositol 4,5-bis-phosphate turnover, 1,2-diacylglycerol and inositol 1,4,5-tris-phosphate formation, and intracellular calcium mobilization. The inhibitory effects of HDL3 on inositol 1,4,5-tris-phosphate formation and intracellular calcium mobilization were abolished after covalent modification of HDL3 with dimethylsuberimidate. Furthermore, they could be blocked by calphostin C and bis-indolylmaleimide, 2 highly selective and structurally unrelated protein kinase C inhibitors. However, the inhibitory effects of HDL3 were not blocked by H89, a protein kinase A inhibitor. In addition, HDL3 failed to induce cAMP formation but stimulated the phosphorylation of the protein kinase C 40- to 47-kD major protein substrate. We observed a close temporal relationship between the HDL3-mediated inhibition of thrombin-induced inositol 1,4,5-tris-phosphate formation, intracellular calcium mobilization, and fibrinogen binding and the phosphorylation of the protein kinase C 40- to 47-kD major protein substrate. Taken together, these findings indicate that the HDL3-mediated inhibition of thrombin-induced fibrinogen binding and aggregation occurs via inhibition of phosphatidylinositol 4,5-bis-phosphate turnover and formation of 1,2-diacylglycerol and inositol 1,4,5-tris-phosphate. Protein kinase C may be involved in this process. 相似文献
63.
64.
MS McNeill DB Griffin TR Dockerty JP Walter HK Johnson JW Savell 《Canadian Metallurgical Quarterly》1998,76(6):1613-1620
Twenty-nine selected styles of subprimals or sections of veal were obtained from a commercial facility to assist in the development of a support program for retailers. They were fabricated into bone-in or boneless retail cuts and associated components by trained meat cutters. Each style selected (n = 6) was used to generate mean retail yields and labor requirements, which were calculated from wholesale and retail weights and processing times. Means and standard errors for veal ribs consisting of five different styles (n = 30) concluded that style #2, 7-rib 4 (10 cm) x 4 (10 cm), had the lowest percentage of total retail yield (P < .05) owing to the greatest percentage of bone. Furthermore, rib style #2 required the longest total processing time (P < .05). Rib styles #3, 7-rib chop-ready, and #5, 6-rib chop ready, yielded the greatest percentage of total retail yield and also had the shortest total processing time (P < .05). Within veal loins, style #2, 4 (10 cm) x 4 (10 cm) loin kidney fat in, had the greatest percentage fat (P < .05). Loin styles #2 and #3, 4 (10 cm) x 4 (10 cm) loin special trimmed, generated more lean and fat trimmings and bone, resulting in lower percentage of total retail yields than loin style #1, 0 (0 cm) x 1 (2.5 cm) loin special trimmed (P < .05). Results indicated that bone-in subprimals and sections required more processing time if fabricated into a boneless end point. In addition, as the number of different retail cuts increased, processing times also increased. 相似文献
65.
Poly(diaryl diazosulfide)s and model diaryl diazosulfides of the general structure aryl-N?N? S-aryl' are tested for their thermostability. Thermolysis in solution is followed by means of UV spectroscopy. At the beginning of the decay most model diazosulfides follow first-order kinetics, but show an acceleration of the decay rate in the further course of the thermolysis. In contrast to the models the decomposition of the corresponding polymers cannot be described by first-order kinetics, except one example ( P 1 ). Thermogravimetry studies show weight losses of 8–12% in the range between 80 and 150°C depending on the structure of the models and polymeric diazosulfides. These values correspond with the content of azo function in these compounds, therefore it is assumed that nitrogen is split off under these reaction conditions. 相似文献
66.
Mandrell D Truong L Jephson C Sarker MR Moore A Lang C Simonich MT Tanguay RL 《Journal of laboratory automation》2012,17(1):66-74
The potential of the developing zebrafish model for toxicology and drug discovery is limited by inefficient approaches to manipulating and chemically exposing zebrafish embryos-namely, manual placement of embryos into 96- or 384-well plates and exposure of embryos while still in the chorion, a barrier of poorly characterized permeability enclosing the developing embryo. We report the automated dechorionation of 1600 embryos at once at 4 h postfertilization (hpf) and placement of the dechorionated embryos into 96-well plates for exposure by 6 hpf. The process removed ≥95% of the embryos from their chorions with 2% embryo mortality by 24 hpf, and 2% of the embryos malformed at 120 hpf. The robotic embryo placement allocated 6-hpf embryos to 94.7% ± 4.2% of the wells in multiple 96-well trials. The rate of embryo mortality was 2.8% (43 of 1536) from robotic handling, the rate of missed wells was 1.2% (18 of 1536), and the frequency of multipicks was <0.1%. Embryo malformations observed at 24 hpf occurred nearly twice as frequently from robotic handling (16 of 864; 1.9%) as from manual pipetting (9 of 864; 1%). There was no statistical difference between the success of performing the embryo placement robotically or manually. 相似文献
67.
阐述了基于B/S模式开发湖南高速铁路职业技术学院财务管理信息系统,并详细介绍了系统的功能需求和数据库设计,以及主要功能模块的设计与实现代码。该系统将Web技术和数据库技术很好的融合在一起,使数据库成为Web的重要组成部分,实现了数据库技术和网络技术的有机结合。 相似文献
68.
任意波形发生器是现代电子测量领域应用最为广泛的一种通用信号源;为了更好地满足人机交互,介绍了一套基于μC/GUI和SOPC技术的任意波形发生器人机交互系统设计方案;系统采用Avalon突发模式设计一个通用的LCD控制器,实现触摸屏为人机交互输入接口,分析并完成μC/GUI的移植,最后完成仪器的图形界面设计;整个系统采用SOPC技术集成到单片FPGA芯片内部,既降低开发成本,又提高系统的稳定性;实验结果表明基于μC/GUI设计的界面简洁友好,触摸屏操作方便直观,整个系统运行稳定,任务能够实时处理;该系统占用资源少,成本低,集成度高,稳定性强,具有一定的实用价值。 相似文献
69.
Gutjahr WJ 《Evolutionary computation》2012,20(3):395-421
For stochastic multi-objective combinatorial optimization (SMOCO) problems, the adaptive Pareto sampling (APS) framework has been proposed, which is based on sampling and on the solution of deterministic multi-objective subproblems. We show that when plugging in the well-known simple evolutionary multi-objective optimizer (SEMO) as a subprocedure into APS, ε-dominance has to be used to achieve fast convergence to the Pareto front. Two general theorems are presented indicating how runtime complexity results for APS can be derived from corresponding results for SEMO. This may be a starting point for the runtime analysis of evolutionary SMOCO algorithms. 相似文献
70.
The microstructure of a commercial cubic boron nitride compact, with aluminium nitride and aluminium diboride binder phases, has been characterized by transmission electron micro-scopy. Within individual grains significant plastic deformation is observed in the form of dis-locations and microtvvin lamellae. There is no evidence of recrystallization. Grains have been forced into direct contact, but there is no evidence of new growth of cubic boron nitride between the grains. Some grains have obviously broken or shattered during manufacture. Aluminium nitride is present mostly as an oriented rind around the cubic boron nitride grains where they are not in direct contact, while aluminium diboride forms a largely continuous network in the channels between them. Our observations suggest that the two aluminium compounds are responsible for most of the bonding in the compact. The orientation relation-ships between the cubic boron nitride and As aluminium nitride rind are, on (1 1 1) facets of BN, BN(1 1 1) AIN(0 0 0 1) and BN[1 1 0] AIN[1 1 – 0], and on (0 0 1) facets of BN, BN(0 0 1) AIN(0 0 0 1) and BN[1 1 0] AIN[l 1 ¨ 0]. The aluminium diboride is mostly unoriented. The results are discussed in the context of previous work on diamond-based compact materials. 相似文献