Nickel-Carnosine complex:A new carrier for enzymes immobilization by affinity adsorption

Immobilization is an effective method to promote the application of enzyme industry for improving the stability and realizing recovery of enzyme.To some extent,the performance of immobilized enzyme depends on the choice of carrier material.Therefore,the development of new carrier materials has been one of the key issues concerned by enzyme immobilization researchers.In this work,a novel organic-inorganic hybrid material,nickel-carnosine complex (NiCar),was synthesized for the first time by solvothermal method.The obtained NiCar exhibits spherical morphology,hierarchical porosity and abun-dant unsaturated coordination nickel ions,which provide excellent anchoring sites for the immobiliza-tion of proteins.His-tagged organophosphate-degrading enzyme (OpdA) and ω-transaminase (ω-TA)were used as model enzymes to evaluate the performance of NiCar as a carrier.By a simple adsorption process,the enzyme molecules can be fixed on the particles of NiCar,and the stability and reusability are significantly improved.The analysis of protein adsorption on NiCar verified that the affinity adsorp-tion between the imidazole functional group on the protein and the unsaturated coordination nickel ions on NiCar was the main force in the immobilization process,which provided an idea way for the develop-ment of new enzyme immobilization carriers.     

面向电力系统潮流仿真的语音交互研究

以深度学习为代表的人工智能技术不断发展,正逐步渗透并融入电力系统等传统领域.为增强潮流仿真中人机交互的智能化程度,减少仿真技术人员在输入数据、计算分析等过程中的重复劳动,本文从语音识别理论和工程应用相结合的角度开展研究,提出了一种在潮流仿真中嵌入语音识别技术的方法.首先,对现有潮流仿真软件融入语音识别技术的可行性进行了探讨;其次,利用基于开源的电力系统分析工具Pandapower,设计了潮流仿真的语音识别流程,提出了相应的实现方法;最后,在建立的语音识别系统上,对1个仿真算例进行了测试.测试结果表明,本文提出在潮流仿真中嵌入语音识别技术的方法,能有效地实现潮流数据的语音编辑以及潮流计算的语音控制等功能.     

Exploring the methods on improving CH4 delivery performance to surpass the Advanced Research Project Ageney-Energy target

CH_4 storage associated with adsorbed natural gas(ANG) technology is an issue attracting great concern.Following the Advanced Research Project Agency-Energy(ARPA-E) targeted deliverable capacity of 315 cm~3·cm~(-3)(STP), hundreds of thousands of materials have been experimentally or theoretically evaluated,while the best results still show a 35% gap from the target. Moreover, recent theoretical research reveals that the target is beyond the possibility that real materials can be designed. To get rid of the awkward situation, we make attempts on investigating the CH_4 delivery performance under other operation conditions. Methods of raising the discharge temperature(to infinite high) or elevating the storage pressure(to 25 MPa) have been proved to show limited effectiveness. In this work, it is found that the ARPA-E target can be achieved by using a decreasing storage temperature strategy. By taking 280 Co RE(computation-ready, experimental) COFs(covalent organic frameworks) as ANG materials, when reduce the storage temperature to 190.6 K, the highest deliverable capacity can reach 392 cm~3·cm~(-3)(STP), and16.1% Co RE COFs can surpass the target. The target is also achievable when storage at 220 K. Structure performance relationships study shows strong correlation between deliverable capacity and void fraction. Hence, 120 hypothetical COFs are generated to ascertain the optimum void fraction. In addition,the performance of 2 D-COFs can be greatly enhanced by increasing the interlayer spacings, e.g. CH_4 deliverable capacity(storage at 190.6 K) of ATFG-COF can be improved from 239 to 411 cm~3·cm~(-3)(STP) when interlayer spacing is enlarged to 1.65 nm.     

 基于混合模型的优化低频低压减载方法研究

文中以低频低压情况下保证电压稳定的前提下减载为研究内容,提出了一种基于混合模型的优化低频低压减载方法。该混合模型将遗传算法与人工神经网络相结合,在保证电压稳定的情况下选择合适的节点进行负荷切除。实验结果表明,该方法可以在低频低压情况下使负荷减载量达到最小。     

基于多分量融合的调度端小电流接地选线策略

针对中性点不接地的配电系统站端接地选线方式投资大、维护成本高,零序电流采样监测运行维护困难的问题,提出一种基于多分量融合的调度端小电流接地选线方法。首先,在现有调度自动化系统监测数据集中,针对性分析了对接地故障较为敏感的单分量特征及其影响因素;其次,综合考虑实际运行可靠性和判断准确性,提出了基于三角模融合的接地选线判据;进而通过归一化计算各线路接地概率,实现在调度端远程的故障线路准确选线;最后,通过在某电力公司实际部署基于所提方法的小电流接地选线功能模块进行实测验证。运行结果显示,所提方法具有良好的选线成功率,能够较好支撑调度端的远程故障定位,具有较高的推广价值。     

便携式样气转移注射器自动清洗装置的研制与应用

进行气相色谱分析,需对转移样气的注射器进行彻底清洗.研制了一款注射器自动清洗装置,代替人工清洗,提高了工作效率,节约了劳动成本,同时避免了人工清洗时人员受伤情况的发生,具有广泛的应用前景.     

SiO2改性聚丙烯纤维棉对油水乳状液的高效分离

近年来,基于特殊润湿性理论制备表面具有微纳米粗糙结构的多孔材料成为油水分离领域研究的重点。为了满足不同环境下对不同形式油-水乳状液高效高通量分离的需求,该研究利用纳米SiO2颗粒对聚丙烯（PP）纤维棉有针对性地亲（疏）水改性,构建了系列不同润湿性和粗糙度的PP纤维棉,探究了不同孔隙度和表面能的PP纤维棉对W/O及O/W型乳状液的分离性能,结果表明,经过亲（疏）水改性后的PP纤维棉对水/正己烷和水/甲苯乳状液的分离效率都高于99.5%,通量高于700 L/(m2·h),并针对不同形式油-水乳状液阐释其相应的分离机制,为后续油-水乳状液分离材料的科学设计和可控制备提供了理论依据。     

Cardiac Oxidative Signaling and Physiological Hypertrophy in the Na/K-ATPase α1s/sα2s/s Mouse Model of High Affinity for Cardiotonic Steroids

The Na/K-ATPase is the specific receptor for cardiotonic steroids (CTS) such as ouabain and digoxin. At pharmacological concentrations used in the treatment of cardiac conditions, CTS inhibit the ion-pumping function of Na/K-ATPase. At much lower concentrations, in the range of those reported for endogenous CTS in the blood, they stimulate hypertrophic growth of cultured cardiac myocytes through initiation of a Na/K-ATPase-mediated and reactive oxygen species (ROS)-dependent signaling. To examine a possible effect of endogenous concentrations of CTS on cardiac structure and function in vivo, we compared mice expressing the naturally resistant Na/K-ATPase α1 and age-matched mice genetically engineered to express a mutated Na/K-ATPase α1 with high affinity for CTS. In this model, total cardiac Na/K-ATPase activity, α1, α2, and β1 protein content remained unchanged, and the cardiac Na/K-ATPase dose–response curve to ouabain shifted to the left as expected. In males aged 3–6 months, increased α1 sensitivity to CTS resulted in a significant increase in cardiac carbonylated protein content, suggesting that ROS production was elevated. A moderate but significant increase of about 15% of the heart-weight-to-tibia-length ratio accompanied by an increase in the myocyte cross-sectional area was detected. Echocardiographic analyses did not reveal any change in cardiac function, and there was no fibrosis or re-expression of the fetal gene program. RNA sequencing analysis indicated that pathways related to energy metabolism were upregulated, while those related to extracellular matrix organization were downregulated. Consistent with a functional role of the latter, an angiotensin-II challenge that triggered fibrosis in the α1^r/rα2^s/s mouse failed to do so in the α1^s/sα2^s/s. Taken together, these results are indicative of a link between circulating CTS, Na/K-ATPase α1, ROS, and physiological cardiac hypertrophy in mice under baseline laboratory conditions.     

光热电站容量效益评估及影响因素研究

光热发电作为一种清洁能源,配置储热后具有良好的调节性能,近年得到大力发展。依据自身经济性,光热电站往往配备较大的储热装置,能够平移所吸收的光热能,且具有跨日调节能力,使得光热机组有一定替代常规机组带负荷的能力,即容量效益。本文提出一种基于等可靠性的光热电站容量效益评估方法,考虑了调峰方式、储热时长、新能源规模、光热电站规模对光热电站容量效益发挥的影响,采用基于数学优化的生产模拟仿真程序,以周为尺度,计算全年8760 h的系统运行状态,计及了机组启停、水电跨日调节、抽蓄跨日调节、光热电站的跨日调节等因素。最后通过对西北地区实际电网的仿真,验证了所提方法的有效性。     

Robust Superhydrophobic Fabric for Durability,Self-Cleaning,and Oil/Water Separation via Thiol–Acrylate Polymerization

In recent years, highly efficient oil/water separation materials have brought much attention. It requests superhydrophobic surfaces with a rapid and facile separation process, excellent durability, and large-scale fabrication. Herein, a facile vapor-liquid sol-gel, and free radical polymerization reaction method to prepare the durable and robust superhydrophobic cotton fabric is proposed. Moreover, the fabric can be used for highly efficient and various oil/water separation. It is prepared via a simple two-step process, including a vapor-liquid sol-gel process to deposit with thiols particles, and then followed a free radical polymerization reaction to graft 2,2,3,4,4,4-hexafluorobutyl methacrylate. Scanning electron microscopy and Fourier transform infrared spectrometry prove that the rough structures are generated from the hydrolysis condensation reaction between tetraethyl orthosilicate and 3-mercaptopropyltriethoxysilane. As a result, the synthetic chemical composition provided by the natural fabric and silica nanoparticles synergistically construct a superhydrophobic surface with water contact angles and shedding angle of 158° and 9°, respectively. Additionally, the treated fabric exhibits excellent chemical resistance and self-cleaning ability. Remarkably, the fabric still retains superhydrophobic and excellent mechanical robustness after 30 cycles of various oil/water separation. In summary, the resultant fabrics with excellent chemical resistance, remarkable mechanical robustness, and versatile separation abilities have potential applications in various oil/water separations.