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71.
Huijbers A Velstra B Dekker TJ Mesker WE van der Burgt YE Mertens BJ Deelder AM Tollenaar RA 《International journal of molecular sciences》2010,11(11):4175-4193
Early diagnosis of cancer is of pivotal importance to reduce disease-related mortality. There is great need for non-invasive screening methods, yet current screening protocols have limited sensitivity and specificity. The use of serum biomarkers to discriminate cancer patients from healthy persons might be a tool to improve screening programs. Mass spectrometry based proteomics is widely applied as a technology for mapping and identifying peptides and proteins in body fluids. One commonly used approach in proteomics is peptide and protein profiling. Here, we present an overview of profiling methods that have the potential for implementation in a clinical setting and in national screening programs. 相似文献
72.
Pamela J. Perez Nicolas Clauvelin Michael A. Grosner Andrew V. Colasanti Wilma K. Olson 《International journal of molecular sciences》2014,15(9):15090-15108
The looping of DNA provides a means of communication between sequentially distant genomic sites that operate in tandem to express, copy, and repair the information encoded in the DNA base sequence. The short loops implicated in the expression of bacterial genes suggest that molecular factors other than the naturally stiff double helix are involved in bringing the interacting sites into close spatial proximity. New computational techniques that take direct account of the three-dimensional structures and fluctuations of protein and DNA allow us to examine the likely means of enhancing such communication. Here, we describe the application of these approaches to the looping of a 92 base-pair DNA segment between the headpieces of the tetrameric Escherichia coli Lac repressor protein. The distortions of the double helix induced by a second protein—the nonspecific nucleoid protein HU—increase the computed likelihood of looping by several orders of magnitude over that of DNA alone. Large-scale deformations of the repressor, sequence-dependent features in the DNA loop, and deformability of the DNA operators also enhance looping, although to lesser degrees. The correspondence between the predicted looping propensities and the ease of looping derived from gene-expression and single-molecule measurements lends credence to the derived structural picture. 相似文献
73.
Therapeutic change involves integration of emotion schemas that have been dissociated. Two types of avoidant dissociation are distinguished: primary dissociation dominated by fragmentary emotional memories; and secondary dissociation involving initial encoding of more organized memories whose meaning is avoided. Reconstruction of dissociated emotion schemas occurs through the referential process which includes three basic components: arousal of the subsymbolic affective core of a dissociated schema in the treatment relationship; connections of subsymbolic processes to symbolic representations in narratives and interactions in the session; and reflection leading to reorganization of the schema. The role of enactive perception and embodied communication as underlying intersubjectivity in the referential process is reviewed. Variations in states of awareness associated with each phase of the process, in both analyst and patient, and their effects on therapeutic change are examined. Current work in cognitive science and affective neuroscience supporting this process model is reviewed. This formulation is largely compatible with Freud's early theory of recollection and “associative working-over” with new emphasis on subjective state and the relational context. Studies of the referential process provide a potential interface between investigations of psychotherapy process and basic cognitive science and neuroscience research. (PsycINFO Database Record (c) 2011 APA, all rights reserved) 相似文献
74.
Koutstaal Wilma; Schacter Daniel L.; Galluccio Lissa; Stofer Kathryn A. 《Canadian Metallurgical Quarterly》1999,14(2):220
Using a categorized pictures paradigm, Koutstaal and Schacter (1997) reported high levels of false recognition of lures that were categorically related to presented items. Although also shown by younger adults, false recognition was markedly higher for older adults. To probe the factors underlying this age difference, these experiments required participants to engage in more careful scrutiny of the items at retrieval or to notice specific differentiating perceptual features of the objects during encoding. False recognition was reduced with each of these manipulations, but neither manipulation, either separately or together, eliminated the age difference in false recognition. Older adults can considerably reduce false recognition if encouraged to use more stringent decision criteria. Persistent difficulty in opposing familiarity-based responding and comparatively more generic encoding may contribute to residual deficits. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
75.
Koutstaal Wilma; Verfaellie Mieke; Schacter Daniel L. 《Canadian Metallurgical Quarterly》2001,15(2):268
Studies have shown lower false recognition of semantically related lure words in patients with global amnesia than in matched controls. This pattern has been interpreted as suggesting that medial temporal and diencephalic structures that are damaged in amnesia and that contribute to veridical memory also contribute to false recognition. It has been argued that whereas controls form and retain a well-organized representation of the semantic gist of studied items, patients with amnesia can retain only a degraded gist representation. However, these studies are subject to an alternative interpretation involving greater source confusions in controls. The authors used a categorized-pictures paradigm to test recognition under conditions in which source confusions were unlikely to occur. Relative to controls, patients with amnesia showed reduced false recognition of categorically related pictorial lures, thereby supporting the notion of degraded gist representations in amnesia. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
76.
Maija Hoikkanen Minna Poikelispää Amit Das Mari Honkanen Wilma Dierkes 《Polymer-Plastics Technology and Engineering》2015,54(4):402-410
In the presence of multiwalled carbon nanotubes (MWCNT), polar nitrile-butadiene rubber (NBR) and nonpolar ethylene propylene diene rubber (EPDM) blends were prepared following a melt mixing method. For the preparation of MWCNT filled EPDM/NBR blends, two mixing methods were used: direct mixing and the masterbatch dilution method. Various physical, mechanical, and morphological properties are explored to elucidate the dispersion behavior of MWCNTs. It was concluded that the preparation method influences the dispersion of the nanotubes in different rubber phases and the properties of these blends are controlled by the degree of dispersion of the nanotubes in the two phases. 相似文献
77.
The articles in this special section have pointed to the need for reexamination of theory and technique, if psychoanalytic treatment is to remain relevant for diverse patient groups. Some clinical assumptions, including both theories of psychosexual development and theories of object relations, and some assumptions as to mutative factors and therapeutic goals, are culture bound in unacknowledged ways. A modern psychological theory of psychic organization and psychic change is needed to account for common factors in the psychoanalytic approach that apply across cultures to enable specific applications for particular patient groups and to serve as a framework for research. The issues of diversity raise a challenge for psychoanalysis but also offer an opportunity to revitalize the field. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
78.
Horowitz Mardi J.; Stinson Charles; Curtis Deborah; Ewert Mary; Redington Dana; Singer Jerome; Bucci Wilma; Mergenthaler Erhard; Milbrath Constance; Hartley Dianna 《Canadian Metallurgical Quarterly》1993,61(3):421
This single-case study examined frank disclosure of important topics in a brief exploratory psychotherapy, including topics closely related to a recent, unintegrated stressor life event. Quantitative measures of emotion and control variables showed heightened levels of both emotionality and defensive control during discourse on the topic of the stressor event. In future studies, such measures of verbal and nonverbal signs of emotional expression and defensive control might be used to identify topics in an unresolved state. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
79.
Janneke Verhaegh Willem F. J. Fontijn Emile H. L. Aarts Wilma C. M. Resing 《Personal and Ubiquitous Computing》2013,17(8):1637-1646
We present a field study with a game for children called ‘Tap the little hedgehog’, which is played on the TagTiles console, a tangible electronic interface. The game was developed to train and assess cognitive skills and includes tasks which, in isolation, exhibit a high correlation with a number of subtests from the Wechsler Intelligence Scale for Children (WISC-III-NL). The tasks address a range of nonverbal skills by requiring children to perform different operations on abstract patterns such as copying, reproducing sequences from memory and mirroring patterns. In the current study, we tested whether these tasks kept their ability to address these skills if included in a gaming context, whether children are able to play the game independently and whether they are motivated to play the game. The results of the study support the hypothesis that nonverbal IQ-scores, as measured by the Wechsler Intelligence Scale for Children, can improve by training with a game. Hence, games like ‘Tap the little hedgehog’ can be used to train specific skills and serve as a screening tool for these skills. The results also confirm that children can play the game independently and that they enjoy it. We further found that children quickly learn how to play the game and use the interface. 相似文献
80.
Numerical weather prediction models play an important role in the field of wind energy, for example, in power forecasting, resource assessment, wind farm (wake) simulations, and load assessment. Continuous evaluation of their performance is crucial for successful operations and further understanding of meteorology for wind energy purposes. However, extensive offshore observations are rarely available. In this paper, we use unique met mast and Lidar observations up to 315 m from met mast “IJmuiden,” located in the North Sea 85 km off the Dutch coast, to evaluate the representation of wind and other relevant variables in three mainstream meteorological models: ECMWF‐IFS, HARMONIE‐AROME, and WRF‐ARW, for a wide range of weather conditions. Overall performance for hub‐height wind speed is found to be comparable between the models, with a systematic wind speed bias <0.5 m/s and random wind speed errors (centered RMSE) <2 m/s. However, the model performance differs considerably between cases, with better performance for strong wind regimes and well‐mixed wind and potential temperature profiles. Conditions characterized by moderate wind speeds combined with stable stratification, which typically produce substantial wind shear and power fluctuations, lead to the largest misrepresentations in all models. 相似文献