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91.
The type I adenylyl cyclase is directly stimulated by Ca2+ and calmodulin in vitro, and the enzyme is also stimulated by increases in intracellular Ca2+ in vivo. Ca2+ stimulation of the enzyme in vivo may be due to direct interactions of the enzyme with Ca2+ and calmodulin or to an indirect mechanism involving stimulation of the enzyme by Ca(2+)-activated protein kinases. In this study, we have made several point mutations within the calmodulin binding domain to determine if the Ca2+ sensitivity of the enzyme can be modified by mutagenesis. The catalytic activities of the mutant enzymes were comparable to wild type type I adenylyl cyclase. Substitution of Cys-507 with Ser-507 did not have significant effects on the calmodulin or Ca2+ sensitivity of the enzyme. However, replacement of Lys-504 with Asp caused a 4-fold decrease in sensitivity to Ca2+. Ca2+ and calmodulin stimulation were abolished by substitution of Phe-503 with Arg-503. Stimulation of type I adenylyl cyclase activity in vivo by intracellular Ca2+ was also greatly diminished with the Arg-503 mutant indicating that Ca2+ stimulation of the enzyme in vivo is due primarily to direct interactions with calmodulin and Ca2+. These data demonstrate that the Ca2+ sensitivity of this enzyme can be modulated by point mutagenesis within the putative calmodulin binding domain and indicate that the enzyme can be directly regulated by Ca2+ and calmodulin in vivo. 相似文献
92.
93.
Krick R.F. Clark L.T. Deleganes D.J. Wong K.L. Fernando R. Debnath G. Banik J. 《Solid-State Circuits, IEEE Journal of》1994,29(12):1455-1463
An implementation of the Pentium microprocessor architecture in 0.6 μm BiCMOS technology is described. Power dissipation is reduced and performance is enhanced over the previous generation. Processor features, implementation technology, and circuit techniques are discussed. An internal clock rate of 150 MHz is achieved at 3.7 V and -55°C 相似文献
94.
This paper examines the detrimental effects of excess majority carriers and photons induced by impact ionization on the operation of neighboring pn junctions, bipolar transistors, MOS transistors, and circuits. The experimental results show that in addition to an increase in the substrate surface potential due to the excess majority carriers, photons can lower the barrier of a pn junction and, as a consequence, shift the Gummel plot of an npn bipolar transistor. As for the neighboring circuits, an example in which the speed of an NMOS ring oscillator is retarded by impact ionization in a neighboring NMOS transistor is presented 相似文献
95.
Parasitic bipolar gain in fully depleted n-channel SOI MOSFET's 总被引:3,自引:0,他引:3
Ver Ploeg E.P. Nguyen C.T. Wong S.S. Plummer J.D. 《Electron Devices, IEEE Transactions on》1994,41(6):970-977
Fully depleted SOI MOSFET's include an inherent parasitic lateral bipolar structure with a floating base. We present here the first complete physically based explanation of the bipolar gain mechanism, and its dependence on bias and technological parameters. A simple, one-dimensional physical model, with no fitting parameters, is constructed, and is shown to agree well with simulations and measurements performed on a new type of SOI MOSFET structure. It is shown that parameters which affect the gain, such as SOI layer thickness, body doping concentration and gate and drain voltages, do so primarily by affecting the concentration of holes in the body region. Thus, current gain falls dramatically with increasing drain voltage due to the associated impact ionization driven increase in the hole concentration. Gummel plots of this parasitic bipolar indicate an apparent ideality factor of 0.5 for the hole current, due to the body hole concentration's dependence on drain voltage 相似文献
96.
High dielectric constant (high-k) polymer composites are of great interest for embedded capacitor applications. Previously,
we demonstrated that epoxy—aluminum composites are promising for embedded capacitor applications, because they have a high
dielectric constant and a low dielectric loss due to the core—shell structure of the self-passivated aluminum particles. In
this work, to further understand the dielectric behavior of aluminum composites, lower-loss polymers such as silicone, polyimide,
polynorbornene, and benzocyclobutene were explored as matrices for the aluminum composites. It is found that the polymer matrices
can significantly change the dielectric properties of the aluminum composites. A polymer matrix with a lower dielectric constant
generally results in a lower dielectric constant of its aluminum composites. In this regard, polymer—aluminum composites have
a similar dielectric characteristic as polymer—ceramic composites. Thermomechanical properties of aluminum composites were
characterized by a thermomechanical analyzer. 相似文献
97.
Kok Wai Wong Chun Che Fung Eren H. Gedeon T. 《IEEE transactions on instrumentation and measurement》2003,52(6):1865-1869
Fuzzy rule-based systems have been very popular in many engineering applications. In mineral engineering, fuzzy rules are normally constructed using some fuzzy rule extraction techniques to establish the determination model in predicting the d50c of hydrocyclones. However, when generating fuzzy rules from the available information, it may result in a sparse fuzzy rule base. The use of more than one input variable is also common in hydrocyclone data analysis. This paper examines the application of fuzzy interpolation to resolve the problems using sparse fuzzy rule bases, and to perform analysis of fuzzy interpolation in multidimensional input spaces. 相似文献
98.
Daniel Won‐Kyu Hong Choong Seon Hong 《International Journal of Network Management》2003,13(2):115-127
This paper proposes a high‐performance connection management architecture to design a common QoS framework applied to an ATM network based on the Open Distributed Processing (ODP) concept. We design the QoS framework in accordance from the RM‐ODP information and computational viewpoints. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
99.
Sang Cheol Lee Won Kook Lee 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》1992,55(3):251-261
The extraction of penicillin G from simulated media was performed by water/oil/water (w/o/w) emulsion liquid membranes (ELMs) and studied under various operational conditions in a batch system. The degree of extraction achieved was between 80% and 95% under specific conditions. A concentration of greater than nine times the initial concentration of penicillin G in the external phase was obtained in the internal phase. The pH of the internal aqueous solution, containing a basic salt, was theoretically calculated on the basis of the amount of penicillin G transported into the internal phase. The calculated results agreed with the experimental data well and were used to select a suitable type and concentration of a basic salt in the internal phase to give a pH within the range 5 to 8 where penicillin G was stable after the termination of extraction. The extraction of penicillin G was successfully performed by the ELM process with sodium carbonate in the internal phase. 相似文献
100.