Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1

Ubiquitin-mediated proteolysis controls the abundance of many cell cycle regulatory proteins. Recent work in Saccharomyces cerevisiae suggests that a complex consisting of Cdc53, Skp1, and a third component known as an F-box protein (termed SCF) in combination with Cdc34 specifically targets regulatory proteins for degradation, and that substrate specificity is likely to be mediated by the F-box subunit. A screen for genetic interactions with a cdc34 mutation yielded MET30, which encodes an F-box protein. MET30 is an essential gene required for cell cycle progression and met30 mutations interact genetically with mutations in SCF components. Furthermore, physical interactions between Met30, Cdc53, Cdc34, and Skp1 in vivo provide evidence for an SCFMet30 complex. We demonstrate the involvement of Met30 in the degradation of the Cdk-inhibitory kinase Swe1. Swe1 is stabilized in met30 mutants and GST-Met30 pull-down experiments reveal that Met30 specifically binds Swe1 in vivo. Furthermore, extracts prepared from cdc34 or met30 mutants are defective in polyubiquitination of Swe1. Taken together, these data suggest that SCF-mediated proteolysis may contribute to the regulation of entry into mitosis. Our data, in combination with previously published results, also provide evidence for distinct SCF complexes in vivo and support the idea that their F-box subunits mediate SCF substrate specificity.     

Unfolding mechanism of rubredoxin from Pyrococcus furiosus

As part of our studies on the structural and dynamic properties of hyperthermostable proteins, we have investigated the unfolding pathways of the small iron-sulfur protein rubredoxin from Pyrococcus furiosus (RdPf) at pH 2. Unfolding has been initiated by temperature jump, triggered by manual mixing of a concentrated protein solution into a thermally preequilibrated buffer. The process has been followed in real time by absorption, tryptophan fluorescence emission, and far-UV circular dichroism. Unlike the case of the mesophilic rubredoxin from Clostridium pasteurianum (RdCp), RdPf displays a complex unfolding kinetics, pointing to the formation of at least three intermediates. All of the steps, including the one involving metal ion release, are extremely slow. However, hydrophobic core relaxation--not Fe3+ loss--is rate-determining for RdPf unfolding. This clearly rules out the fact that Fe3+ is solely responsible for the kinetic stability of RdPf. Results have been discussed in terms of sequential vs parallel pathways, and the possible role of irreversible phenomena has been taken into consideration. Aggregation does not appear to play a significant role in the observed kinetic complexities. According to a proposed sequential mechanism, partial release of secondary structure elements precedes iron loss, which is then followed by further loss of beta-sheet content and, finally, by hydrophobic relaxation. Although the main features of the RdPf unfolding mechanism remain substantially unchanged over the experimentally accessible temperature range, final hydrophobic relaxation gets faster, relative to the other events, as the temperature is decreased. A qualitative assessment of the unfolding activation parameters suggests that this arises from the very low activation energies (Ea) that characterize this step.     

苏州市景观水体表观污染类型识别及特征指标筛选

为解决表观污染成因的问题,以苏州市区河网为主要研究对象,采集796个数据样本,对景观水体的表观污染类型进行划分界定,并通过对数据样本的统计分析,探究水体表观污染类型和水质指标之间的关系。结果表明:氧化还原电位、溶解氧、叶绿素a、高锰酸盐指数和浊度对不同污染类型的水体具有很好的区分度,可作为主要特征指标;景观水体可分为有机主导型、无机主导型、营养主导型和混合型4种表观污染类型;不同表观污染水体判别的优先次序为有机主导型、无机主导型、营养主导型、混合型。     

采用工作量证明共识机制的区块链中挖矿攻击者间的“鲶鱼效应”

近年来,采用工作量证明共识机制(Proof of Work,PoW)的区块链被广泛地应用于以比特币为代表的数字加密货币中.自私挖矿攻击(Selfish mining)等挖矿攻击(Mining attack)策略威胁了采用工作量证明共识机制的区块链的安全性.在自私挖矿攻击策略被提出之后,研究者们进一步优化了单个攻击者的挖矿攻击策略.在前人工作的基础上,本文提出了新颖的两阶段挖矿攻击模型,该模型包含拥有单攻击者的传统自私挖矿系统与拥有两个攻击者的多攻击者系统.本文的模型同时提供了理论分析与仿真量化分析,并将两个攻击者区分为内部攻击者与外部攻击者.通过引入内部攻击者与外部攻击者的概念,本文指出传统自私挖矿系统转化为多攻击者系统的条件.本文进一步揭示了在多攻击者系统中两个攻击者将产生竞争并面临着“矿工困境”问题.攻击者间的竞争可被总结为“鲶鱼效应”:外部攻击者的出现导致内部攻击者的相对收益下降至多67.4%,因此内部攻击者需要优化攻击策略.本文提出了名为部分主动发布策略的全新挖矿攻击策略,相较于自私挖矿策略,该策略是半诚实的攻击策略.在特定场景下,部分主动发布策略可以提高攻击者的相对收益并破解攻击者面临的“矿工困境”问题.     

手机数据存储和恢复方法研究

文章以手机数据存储和恢复为研究目的,对目前主流的手机操作系统,即Java非智能手机操作系统、塞班非智能与智能手机操作系统,分别将其数据的删除和恢复等进行对比分析,总结数据存储结构和存储方式,归纳手机数据恢复的方法,以期有效解决日常数据丢失等相关问题。     

Measurements and analysis of transmitted spectra from LOTUS fission-suppressed hybrid blanket driven by D-T neutrons

The neutron spectra transmitted across a fission-suppressed hybrid blanket and its components, driven by a low intensity 14 MeV Haefely neutron generator, were measured with a 2×2 NE213 detector at LOTUS facility. These experiments have been analyzed with 2D and 3D codes DOT3.5 and MCNP, respectively. The spectral integrals between 15 to 1 MeV show good agreement among the 2D, the 3D, and the NE213 for 15 cm lead, 18 cm beryllium, and 25 cm graphite slabs. However, there are large discrepancies for 6.2 cm stainless steel and 15 cm lithium carbonate slabs. The assemblies involving two or more of these slabs reflect these tendencies. We observe also considerable disagreement over pointwise spectra for a number of assemblies.Work done while on visiting assignment to Institut de Génie Atomique, E.P.F.L.     

基于互联网实现多种信息传输技术

随着信息技术的迅速发展,使用互联网实现楼宇内电话、电视、电脑、安防和环境监控、计量数据等信息的合路传输,是实现智能家居和数字楼宇的一种主要发展趋势。最终目的,实现对物和过程的智能化感知。文章首先介绍信息合路编码、中央控管电路,然后介绍网络接口电路原理和系统体系构架。重点介绍多种多样的信息传输技术。它对现代楼宇信息化、智能化建设有很好的参考作用。     

基于混合加权的双门限能量协作检测算法研究

研究无线电系统能量优化问题,由于路径损耗导致检测到的信号能量值减小,信号干扰增大,门限值减小,从而使双门限能量协作检测算法的检测概率降低。针对此问题提出混合自适应加权双门限检测算法。首先利用两个门限值将认知用户检测到的能量值划分为不同的区域,然后对位于两个门限值之间的认知用户检测能量值引入信噪比权重因子和距离权重因子进行混合加权,依此来自适应调整其双门限权值,以减小能量损耗较大的信号对门限值的影响,最后融合中心采用"或"准则做出最终的检测结果。仿真结果表明混合自适应加权双门限检测算法比传统等权值的双门限能量协作检测算法具有更高的检测概率,使通信系统能量检测准确性大大提高。     

光伏电站远程监控系统设计

本文主要论述分散式光伏电站远程监控系统的设计与实现。远程监控系统主要由基于B/S的三层体系结构主站、基于ARM的智能终端以及基于单片机的数据采集装置组成。数据采集装置主要采集光伏电站的电压电流等信息,通过RS232串口传给智能终端;智能终端分析处理采集的数据,通过GSM短信机制和主站通信,从而实现光伏电站的远程监控。     

基于泊肃叶方程改进的驱替面积确定方法

为解决B-L水驱前缘理论中前缘推进位置的准确度有限问题,基于泊肃叶方程,通过联立油藏物质平衡方程,转化为地层压力与流管数的最小化问题,运用遗传算法寻优最小目标值,获取各注采方向的流管数,从而确定注采井间驱替面积.该模型量化了B-L方程中驱替面积参数取值,利用改进后的井间驱替面积计算公式,提高了连通的注采井之间水驱前缘推进位置的计算精度.计算结果更加符合油田实际生产,对类似油田的水驱开发效果评价,具有一定的借鉴和指导意义.