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103.
该文报道了一种基于酶级联扩增的电化学核酸适体检测腺苷的新方法。当腺苷存在时,滚环扩增的引物通过E.coli DNA连接酶的作用与通过巯基组装在金电极上的固定探针连接。在pHi29 DNA聚合酶的作用下,滚环扩增反应进行并产生一条与环形探针完全互补的长的单链,然后将大量金纳米粒子标记的核酸探针与滚环扩增的产物杂交。该传感界面电化学行为的结果表明,通过酶级联扩增的方法提高了检测腺苷的阻抗响应灵敏度,且选择性和重现性良好。用于腺苷的检测时,其线性范围为2.0μmol/L~100μmol/L,最低检测浓度为2.0μmol/L,表明该传感界面的设计可作为一种通用方法而有望用于其它目标物的检测。 相似文献
104.
闫锦秀 《网络安全技术与应用》2013,(11):13-14
随着高新科学技术的发展,互联网的规模也不断扩大.人们对互联网需求也越来越多,而现有的网络体系结构开始难以满足人们的需求.本文主要通过讨论现今网络体系结构存在的问题,对相关技术进行研究. 相似文献
105.
This paper addresses the design of low‐level controllers for leader–follower formations of nonholonomic vehicles in the presence of bounded measurement delays. The concept of input‐to‐state stability is extended to encompass the effect of bounded delays and restrictions on the input. A method is proposed to integrate a Smith predictor in a backstepping design on the basis of nested saturations and nonlinear small‐gain assignment, which allows for time delays in the feedback loop. Robustness analysis under uncertain bounded time delays is provided, and design tradeoffs resulting from the use of bounded controls are discussed. Illustrative simulations are shown to validate the design and robustness analysis in the context of a simple leader–follower trailing control problem. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
106.
This paper proposes an improved semi-fragile speech watermarking scheme by quantization of linear prediction (LP) parameters, i.e., the inverse sine (IS) parameters. The spectral distortion due to watermark embedding is controlled to meet the ‘transparency’ criterion in speech coding. A modified bit allocation algorithm combined with watermarking is developed to determine the quantization step so that the ‘transparency’ requirement is satisfied. Due to the statistical nature, the LP coefficients estimated from the watermarked speech signal are different from the watermarked LP coefficients even in the absence of attacks. This effect is the cause of increase in decoding error and minimum authentication length. To tackle this problem, an Analysis by Synthesis (AbS) scheme is developed to reduce the difference between the estimated LP coefficients and the watermarked ones. The watermark detection threshold and minimum authentication length are then derived according to the probability of error and the signal to noise ratio (SNR) requirements. Experimental results show that the proposed AbS based method can effectively reduce the difference between the watermarked IS parameter and the extracted IS parameter when there is no attacks. In addition, the modified bit allocation algorithm can automatically find the appropriate quantization step used in the odd-even modulation so that the transparency requirement is satisfied. 相似文献
107.
针对片状颗粒厚度检测的实际需求,结合机器视觉、图像处理和嵌入式技术,设计与实现了一套基于高性能ARM11的微片状颗粒厚度检测系统。该系统以QT和S3C6410为软硬件平台,提出了利用轮廓提取和最小矩形边界框相结合的方法计算片状颗粒厚度。结果表明,该检测系统具有处理速度快,测量精度高和成本低廉等优点,满足了对颗粒厚度测量的需求。 相似文献
108.
Various molecular docking software packages are available for modeling interactions between small molecules and proteins.However,there have been few reports of modeling the interactions between metal ions and metalloproteins.In this study,the AutoDock package was employed to example docking into a di-iron binding protein,bacterioferritin.Each binding site of this protein was tested for docking with iron ions.Blind docking experiments showed that all docking conformations converged into two clusters,one for internal iron binding in sites within the metalloprotein and the other for external iron binding on the protein surface.Local docking experiments showed that there were significant differences between two internal iron binding sites.Docking at one site gave a reasonable root-mean-square deviation(RMSD) distribution with relatively low binding energy.Analysis of the binding mode quality for this site revealed that more than half of the docking conformations were categorized as having good binding geometry,while no good conformations were found for the other site.Further investigations indicated that coordinating water molecules contributed to the stability of binding geometries.This study provides an empirical approach towards the study of molecular docking in metalloproteins. 相似文献
109.
施工企业的发展依赖于先进的企业管理,现代施工企业管理的有效工具是价值管理及业绩评价.施工企业传统的业绩评价一般以已经发生的经济业务及其成果作为衡量依据,具有短期性和滞后性;真正有价值的业绩评价应当面向企业的战略计划和未来发展方向,在企业业绩评价和考核中引入价值管理理念,实现企业价值最大化. 相似文献
110.
本文以福生水电站设计的坝型选择为例,综合阐速了利用大中型水电站发电尾水,选择适合的坝型建低水头大流量小水电站具有可观的前景. 相似文献