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11.
We examined the molecular mechanism of metronidazole resistance by constructing a lambda-Zap II phagemid expression library with genomic DNA from a metronidazole-resistance strain of Helicobacter pylori. Twenty-two clones were found to have elevated MTZ resistances in XLOLR strain of E. coli. Phagemids belonging to the twenty two clones were extracted and then retransformed into the XLOLR strain of E. coli. After MTZ selection, five clones could confer metronidazole resistance consistently. According to Southern hybridization and DNA sequencing, the five clones contained a same locus, recA. In addition, transforming the five clones into BL21 strain of E. coli produced a higher resistance to MTZ. Interestingly, electroporation of one of the five phagemid clones into two MTZ sensitive H. pylori yielded MTZ resistant strains. Comparing amino acid sequence in MTZ resistant with sensitive isolates revealed two point mutations at this locus. Above results suggest that mutation in recA may be associated with metronidazole resistance of H. pylori. 相似文献
12.
DK Bhuyan X Huang G Kuriakose WH Garner KC Bhuyan 《Canadian Metallurgical Quarterly》1997,16(6):519-526
This article takes issue with procedural reductionism, which is the inclination to reduce all matters of judgement and responsibility to the following of some procedure or rule. Two scenarios provide content for a discussion of professional discretion in the context of accountability. The author shows that in professional life there will always be situations that stand beyond the rules of procedures and require the unique judgement of the professional at the time. While this judgement may be determined by the facts available in a situation of uncertainty, it cannot be reduced to the facts (including facts about rules and procedures). The moral judgement will still have an essential indeterminancy about it. 相似文献
13.
A technique for SiO2 formation by liquid-phase deposition (LPD) at nearly room temperature for low-temperature processed (LTP) polysilicon thin-film transistor (poly-Si TFT) was developed. LPD SiO2 film with a lower P-etch rate shows a dense structure. LPD SiO2 also exhibits good electrical characteristics. LTP poly-Si thin-film transistors (TFTs) with LPD SiO 2 as the gate insulator have been fabricated and investigated. Their characteristics indicate performance adequate for their use as pixel transistors in liquid crystal displays (LCDs) 相似文献
14.
15.
TY Chen ST Lee TN Lui CW Wong YS Yeh WC Tzaan SY Hung 《Canadian Metallurgical Quarterly》1997,48(5):435-40; discussion 441
BACKGROUND: Controversy surrounds the treatment of traumatic central cord syndrome (TCCS), as there are strong advocates for nonsurgical treatment for most patients. However, conservative treatment has been shown to yield a longer period of discomfort from pain and weakness in certain cases. METHODS: In a retrospective review of 114 patients presenting with acute or chronic TCCS from 1988-94, four different age groups were separately observed under different treatments. Motor and sensory recovery were assessed. RESULTS: Better results were achieved in younger patients, with or without radiographic abnormalities, and in patients with clinically correlated encroaching cord lesions who received early surgical decompression. CONCLUSIONS: Surgical intervention for TCCS must be addressed with careful clinical and radiographic survey. Removal of offending lesions in the subacute period results in significant motor and sensory improvement in short-term and long-term follow-up. 相似文献
16.
This study focuses on how to assess the optimal environmental investment decisions under economic and ecological uncertainty, and establishes the continuous time model using the real option approach to optimize environmental pollution policy. Unlike traditional cost benefit analysis, this work extends the model of [Pindyck, R.S., 2002. Optimal timing problems in environmental economics. Journal of Economic Dynamics and Control 26(9–10), 1677–1697], and attempts to identify the storage threshold of pollution stocks and the optimal timing for implementing environmental pollution decisions. 相似文献
17.
Lianshan Yan Yeh C. Yang G. Lin L. Chen Z. Shi Y.Q. Willner A.E. Yao X.S. 《Lightwave Technology, Journal of》2003,21(7):1676-1684
We demonstrate the first programmable group-delay module based on polarization switching. With a unique binary tuning mechanism, the device can generate any differential group delay value from -45 to +45 ps with a resolution of 1.40 ps, or any true-time-delay value from 0 to 45 ps with a resolution of 0.7 ps. The delay varying speeds for both applications are under 1 ms and can be as fast as 0.1 ms. We evaluate both the dynamic and static performances of the device while paying special attention to its dynamic figures of merit for polarization-mode dispersion emulation and compensation applications. Our experiment shows that the device exhibits a negligible transient-effect induced power penalty (<0.2 dB) in a 10-Gb/s nonreturn-to-zero system. 相似文献
18.
Ho-Ming Yeh 《Chemical Engineering Communications》2002,189(10):1360-1367
Effects of inclined angle and plate spacing on the degree of separation in batch-type flat-plate thermal diffusion columns, with operating expense and fixed charge kept unchanged, are discussed. It was found that the degree ofseparation increases when the inclined angle increases or when the platespacing decreases. However, decreasing the plate spacing will lead to decreasingthe amount of solution to be treated in the column, while increasing the inclined angle will enlarge the operating time for reaching the steady state. 相似文献
19.
MT Yeh TN Mather RT Coughlin C Gingrich-Baker JW Sumner RF Massung 《Canadian Metallurgical Quarterly》1997,35(4):944-947
A new indirect fluorescent-antibody (IFA) assay with antigen produced in vitro in the human promyelocytic leukemia cell line HL60 was used to identify the first recognized case of human granulocytic ehrlichiosis in Rhode Island. This IFA assay was used to detect granulocytic ehrlichiae in white-footed mice and in a dog inhabiting the area surrounding the patient's residence. Host-seeking Ixodes scapularis ticks found in the same habitat also were infected. I. scapularis ticks collected from other locations were fed on dogs and New Zealand White rabbits to assess the competency of these species as hosts of granulocytotropic Ehrlichia. Tick-induced infections of dogs were confirmed by serologic testing, tissue culture isolation, and PCR amplification, whereas several rabbits seroconverted but were PCR and culture negative. PCR amplification of the 16S rRNA gene and DNA sequencing of the PCR products or culture isolation was used to confirm granulocytic Ehrlichia infections in humans, dogs, white-footed mice, and ticks. 相似文献
20.
The dc and ac electrical conduction properties have been investigated in four composites containing hydroxyl-terminated polybutadiene (HTPB) as binder and different proportions of aluminum particles as filler in a temperature range of -60 to +70°C and a frequency range of 40 Hz to 100 kHz. A change in conduction mechanism at a temperature of 4±3°C was displayed explicitly in de measurements. The transition was shown to be due to crystallization of the HTPB upon cooling. dc conduction is explained in terms of ionic hopping in HTPB. Distinct polarization mechanisms, below and above the transition temperature, have been identified as interfacial polarisation and electrode blocking effect, respectively. The change of conduction behaviors at the crystallization temperature, 4±3°C, was not revealed explicitly in ac measurements. However, using the free volume concept to analyze the frequency shift factor aT, a discontinuity in the fractional free volume curve was displayed. The temperature of the break point, 6.7°C, corresponds to the crystallization temperature of HTPB. The study showed that the crystallization of a polymer is accompanied by an abrupt reduction in free volume, and this structural change affects dc as well as ac conduction behaviors 相似文献