Spectroscopic evaluation of the effect of a red microalgal polysaccharide on herpes-infected Vero cells

The sulfated polysaccharide obtained from a species of red microalga has proved to be a potent antiviral agent against various members of the herpes family. In the present study, we used microscopic Fourier transform infrared spectroscopy (FT-IR) to investigate differences between normal cells, those infected with herpes viruses, and infected cells treated with red microalgal polysaccharide. FT-IR enables the characterization of cell or tissue pathology based on characteristic molecular vibrational spectra of the cells. The advantage of microscopic FT-IR spectroscopy over conventional FT-IR spectroscopy is that it facilitates inspection of restricted regions of cell cultures or tissue. Our results showed significant spectral differences at early stages of infection between infected and noninfected cells, and between infected cells treated with the polysaccharide and those not treated. In infected cells, there was an impressive decrease in sugar content and a considerable increase in phosphate levels in conjunction with the infection progress. Our results also proved that sugars penetrated and accumulated inside cells treated with the red microalgal polysaccharide. These could have been sugar fragments of low molecular weight present in the polysaccharide solution, despite purification by dialysis. Such sugar accumulation might be responsible for a breakdown in the internal steps of the viral replication cycle.     

Evaluating drought response of Southern Cape Indigenous Forests,South Africa,using MODIS data

Indigenous forests in South Africa cover less than 0.5% of the total land area but are a valued resource under threat from fragmentation, fires, exploitation, and climate change. The largest indigenous forest complex is located along the southern coast of the Western Cape Province. This complex is made up of sub-forests distinguished by different structural and edaphoclimatic attributes. It has been hypothesized that these sub-forests exhibit different resistance to stressors, such as drought. A time series of MODIS 250 m enhanced vegetation index (EVI) data were used to characterize the foliage condition of the three distinctive sub-forests before, during, and after a severe drought in 2009. The goal was to determine how these sub-forests responded to this disturbance. EVI anomalies for the drought and post-drought periods were calculated using annual median EVI values, since removal of outliers based on quality control flags that accompany the MODIS products or noise-filtering techniques proved to be ineffective. Results of the study indicated that pre-drought foliage density EVI was not controlled by differences in water availability, but may have been due to other edaphoclimatic or structural attributes. Maximum foliage loss occurred one year after the driest year, indicating the cumulative effects of drought stress on forest production and retention of foliage. The hypothesized stress resistance capacity of the three sub-forests was found to correspond to their rate of post-drought recovery. There is a need to tie these satellite observations of forest drought response to ground observations of forest condition, growth, and specific site attributes.     

State governance evolution in resource-rich transition economies: An application to Russia and Kazakhstan

Following a decade of transition in the Former Soviet Union (FSU), governance of the oil and gas sectors has evolved to economic nationalism. In the newly independent states this has manifested itself through greater (direct) state ownership or participation in oil and gas production, at the expense of both domestic (in the case of Russia) and international oil companies, as well as legislative developments that increase the flow of oil and gas value to the state. Here we analyse some of the dynamics giving rise to economic nationalism within a model of a state capacity and the ability to implement policy and extract value. Our analysis is based on the institutional and economic functioning of the oil and gas sector. We analyse a vector of institutions and examine Production Sharing Agreements and National Oil Companies.     

The use of RO to remove emerging micropollutants following CAS/UF or MBR treatment of municipal wastewater

The removal of various organic micropollutants (OMPs), including six antibiotics (ERY, ROX, CLA, SMX, SMZ, and TMP), three pharmaceuticals (ibuprofen, salicylic acid, and diclofenac), one industrial product (BPA), and one hormone (cholesterol), was investigated in two pilot plants treating the same raw sewage of the Tel-Aviv WWTP. The effluent production by CAS-UF was 45 m³/h while that of MBR was 40 L/h. Each system's effluent constituted the feed for its RO, which comprised three RO steps after the CAS/UF and a semi-batch RO system after the MBR. Despite significant molecular differences between the selected OMPs, high removal rates were achieved after the RO stage (> 99% for macrolides, pharmaceuticals, cholesterol, and BPA, 95% for diclofenac, and > 93% removal of sulfonamides). However, low antibiotics concentrations and 28–223 ng/L residuals of ibuprofen, diclofenac, salicylic acid, cholesterol, and BPA in the MBR/RO and CAS-UF/RO permeates showed that although RO is an efficient removal solution, it cannot serve as an absolute barrier to OMPs. Therefore, additional treatment techniques should be considered to be incorporated aside the RO to ensure complete removal of such substances.     

Cooperative multihop broadcast for wireless networks

We address the minimum-energy broadcast problem under the assumption that nodes beyond the nominal range of a transmitter can collect the energy of unreliably received overheard signals. As a message is forwarded through the network, a node will have multiple opportunities to reliably receive the message by collecting energy during each retransmission. We refer to this cooperative strategy as accumulative broadcast. We seek to employ accumulative broadcast in a large scale loosely synchronized, low-power network. Therefore, we focus on distributed network layer approaches for accumulative broadcast in which loosely synchronized nodes use only local information. To further simplify the system architecture, we assume that nodes forward only reliably decoded messages. Under these assumptions, we formulate the minimum-energy accumulative broadcast problem. We present a solution employing two subproblems. First, we identify the ordering in which nodes should transmit. Second, we determine the optimum power levels for that ordering. While the second subproblem can be solved by means of linear programming, the ordering subproblem is found to be NP-complete. We devise a heuristic algorithm to find a good ordering. Simulation results show the performance of the algorithm to be close to optimum and a significant improvement over the well known BIP algorithm for constructing energy-efficient broadcast trees. We then formulate a distributed version of the accumulative broadcast algorithm that uses only local information at the nodes and has performance close to its centralized counterpart.     

Determination of Six Paraben Residues in Fresh-cut Vegetables Using QuEChERS with Multi-walled Carbon Nanotubes and High-Performance Liquid Chromatography–Tandem Mass Spectrometry

In this study, an optimized QuEChERS sample preparation method was developed to analyze residues of six parabens: methyl-, ethyl-, n-propyl-, isopropyl-, n-butyl-, and isobutylparaben in five fresh-cut vegetables (potato, broccoli, carrot, celery, and cabbage) with high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). Homogenized samples were extracted using acetonitrile, and the extracts were cleaned with the novel sorbent multi-walled carbon nanotubes (MWCNTs). MWCNTs provided 84–94% removal of chlorophyll and lower matrix effects (MEs) compared to commonly used primary-secondary-amine (PSA) sorbent. Selected parabens were separated by HPLC with isocratic elution using acetonitrile and 0.1% (v/v) formic acid solution and determined by triple quadrupole MS/MS. The method validation results showed that recoveries were at 70–120% with RSDs <20%. Calibration curves showed linear responses of six parabens with R ² > 0.99. Fifty fresh-cut vegetable samples from different farmer markets in Beijing, China were collected to measure the paraben residues, and only one sample was tested positive with methylparaben concentration at 81 μg/kg.     

Remodeling of chromatin loops does not account for specification of replication origins during Xenopus development

The right mammary gland of 12 lactating goats was inoculated intracisternally with 1 ml of Mycoplasma capricolum subsp. capripneumoniae (Mcc) containing 10(6) colony-forming units (CFU), while their left mammary halves received 1 ml of sterile PPLO broth only. Two goats served as uninfected controls. The clinical mastitis that developed in the infected mammary halves within 24 h was initially acute but became increasingly chronic by the end of the experiment at 24 days post inoculation (DPI). The disease was characterized by atrophy of the infected mammary halves, leading to marked agalactia and an increase in somatic cell counts, with a preponderance of neutrophils initially and lymphocytes later. The Mycoplasma was re-isolated from infected mammary secretions up to 16 DPI but not from blood. Histopathology revealed that the mastitis was acute and purulent initially, followed by infiltration of lymphonuclear cells and fibroplasia in the lymphomononuclear cells and fibroplasia in the interacinar tissue, and later by massive fibrosis. Immunohistology demonstrated the presence of Mycoplasma-like bodies localized mainly on the surface of acinar/duct epithelial cells. The studies showed that Mcc was highly pathogenic in the caprine mammary gland.