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61.
Novel biomineralized hydrogels composed of hyaluronic acid (HA) and vinyl phosphonic acid (VPAc) were designed with the aim of developing a biomimetic hydrogel system to improve bone regeneration by local delivery of a protein drug including bone morphogenetic proteins. We synthesized crosslinked hydrogels composed of methacrylated HA and poly(VPAc) [P(VPAc)], which serves as a binding site for calcium ions during the mineralization process. The HA/P(VPAc) hydrogels were biomineralized by a urea‐mediation method to create functional polymer hydrogels that can deliver the protein drug and mimic the bone extracellular matrix. The water content of the hydrogels was influenced by the HA/P(VPAc) composition, crosslinking density, biomineralization, and ionic strength of the swelling media. All HA/P(VPAc) hydrogels maintained more than 84% water content. Enzymatic degradation of HA/P(VPAc) hydrogels was dependent on the concentration of hyaluronidase and the crosslinking density of the polymer network within the hydrogel. In addition, the release behavior of bovine serum albumin from the HA/PVPAc hydrogels was mainly influenced by the drug loading content, water content, and biomineralization of the hydrogels. In a cytotoxicity study, the HA/P(VPAc) and biomineralized HA/P(VPAc) hydrogels did not significantly affect cell viability. These results suggest that biomineralized HA/P(VPAc) hydrogels can be tailored to create a biomimetic hydrogel system that promotes bone tissue repair and regeneration by local delivery of protein drugs. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 41194. 相似文献
62.
The objectives of this study were twofold. The first was to investigate the compressive and flexural behaviors of small‐sized concrete specimens strengthened with sprayed fiber reinforced polymer (SFRP), considering various fiber lengths, fiber volumes, and concrete strengths. The second was to evaluate the strengthening effects of SFRP on the flexural behaviors of large‐sized reinforced concrete beams. U‐shaped strips and shear keys were applied to the test specimens to improve the interfacial bond resistance between the concrete surface and SFRP. POLYM. COMPOS., 36:722–730, 2015. © 2014 Society of Plastics Engineers 相似文献
63.
Yeon-Mi Hong Seo Yeon Min Dayeong Kim Subin Kim Daekwan Seo Kyoung Hwa Lee Sang Hoon Han 《International journal of molecular sciences》2022,23(5)
Attenuating the expression of immediate early (IE) proteins is essential for controlling the lytic replication of human cytomegalovirus (HCMV). The human microRNAs (hsa-miRs), miR-200b-3p and miR-200c-3p, have been identified to bind the 3′-untranslated region (3′-UTR) of the mRNA encoding IE proteins. However, whether hsa-miRs can reduce IE72 expression and HCMV viral load or exhibit a crosstalk with the host cellular signaling machinery, most importantly the NF-κB cascade, has not been evaluated. In this study, argonaute-crosslinking and immunoprecipitation-seq revealed that miR-200b-3p and miR-200c-3p bind the 3′-UTR of UL123, which is a gene that encodes IE72. The binding of these miRNAs to the 3′-UTR of UL123 was verified in transfected cells stably expressing GFP. We used miR-200b-3p/miR-200c-3p mimics to counteract the downregulation of these miRNA after acute HCMV infection. This resulted in reduced IE72/IE86 expression and HCMV VL during lytic infection. We determined that IE72/IE86 alone can inhibit the phosphorylation of RelA/p65 at the Ser536 residue and that p-Ser536 RelA/p65 binds to the major IE promoter/enhancer (MIEP). The upregulation of miR-200b-3p and miR-200c-3p resulted in the phosphorylation of RelA/p65 at Ser536 through the downregulation of IE, and the binding of the resultant p-Ser536 RelA/p65 to MIEP resulted in a decreased production of pro-inflammatory cytokines. Overall, miR-200b-3p and miR-200c-3p—together with p-Ser536 RelA/p65—can prevent lytic HCMV replication during acute and latent infection 相似文献
64.
Lubna Sajjad Asaf Rahmatullah Jan Abdul Latif Khan Waqar Ahmad Saleem Asif Ahmed Al-Harrasi Kyung-Min Kim In-Jung Lee 《International journal of molecular sciences》2022,23(5)
The mechanism and course of Triticum plastome evolution is currently unknown; thus, it remains unclear how Triticum plastomes evolved during recent polyploidization. Here, we report the complete plastomes of two polyploid wheat species, Triticum sphaerococcum (AABBDD) and Triticum turgidum subsp. durum (AABB), and compare them with 19 available and complete Triticum plastomes to create the first map of genomic structural variation. Both T. sphaerococcum and T. turgidum subsp. durum plastomes were found to have a quadripartite structure, with plastome lengths of 134,531 bp and 134,015 bp, respectively. Furthermore, diploid (AA), tetraploid (AB, AG) and hexaploid (ABD, AGAm) Triticum species plastomes displayed a conserved gene content and commonly harbored an identical set of annotated unique genes. Overall, there was a positive correlation between the number of repeats and plastome size. In all plastomes, the number of tandem repeats was higher than the number of palindromic and forward repeats. We constructed a Triticum phylogeny based on the complete plastomes and 42 shared genes from 71 plastomes. We estimated the divergence of Hordeum vulgare from wheat around 11.04–11.9 million years ago (mya) using a well-resolved plastome tree. Similarly, Sitopsis species diverged 2.8–2.9 mya before Triticum urartu (AA) and Triticum monococcum (AA). Aegilops speltoides was shown to be the maternal donor of polyploid wheat genomes and diverged ~0.2–0.9 mya. The phylogeny and divergence time estimates presented here can act as a reference framework for future studies of Triticum evolution. 相似文献
65.
Ji Yeon Noh Chia-Shan Wu Jennifer A. A. DeLuca Sridevi Devaraj Arul Jayaraman Robert C. Alaniz Xiao-Di Tan Clinton D. Allred Yuxiang Sun 《International journal of molecular sciences》2022,23(4)
Chronic low-grade inflammation is a hallmark of aging, which is now coined as inflamm-aging. Inflamm-aging contributes to many age-associated diseases such as obesity, type 2 diabetes, cardiovascular disease, and inflammatory bowel disease (IBD). We have shown that gut hormone ghrelin, via its receptor growth hormone secretagogue receptor (GHS-R), regulates energy metabolism and inflammation in aging. Emerging evidence suggests that gut microbiome has a critical role in intestinal immunity of the host. To determine whether microbiome is an integral driving force of GHS-R mediated immune-metabolic homeostasis in aging, we assessed the gut microbiome profiles of young and old GHS-R global knockout (KO) mice. While young GHS-R KO mice showed marginal changes in Bacteroidetes and Firmicutes, aged GHS-R KO mice exhibited reduced Bacteroidetes and increased Firmicutes, featuring a disease-susceptible microbiome profile. To further study the role of GHS-R in intestinal inflammation in aging, we induced acute colitis in young and aged GHS-R KO mice using dextran sulfate sodium (DSS). The GHS-R KO mice showed more severe disease activity scores, higher proinflammatory cytokine expression, and decreased expression of tight junction markers. These results suggest that GHS-R plays an important role in microbiome homeostasis and gut inflammation during aging; GHS-R suppression exacerbates intestinal inflammation in aging and increases vulnerability to colitis. Collectively, our finding reveals for the first time that GHS-R is an important regulator of intestinal health in aging; targeting GHS-R may present a novel therapeutic strategy for prevention/treatment of aging leaky gut and inflammatory bowel disease. 相似文献
66.
Jong-Min Park Mi Yeon Kim Joachim Jose Min Park 《International journal of molecular sciences》2022,23(1)
A regenerable immunoaffinity layer comprising covalently immobilized orientation-controlled antibodies was developed for use in a surface plasmon resonance (SPR) biosensor. For antibody orientation control, antibody-binding Z-domain-autodisplaying Escherichia coli (E. coli) cells and their outer membrane (OM) were utilized, and a disuccinimidyl crosslinker was employed for covalent antibody binding. To fabricate the regenerable immunoaffinity layer, capture antibodies were bound to autodisplayed Z-domains, and then treated with the crosslinker for chemical fixation to the Z-domains. Various crosslinkers, namely disuccinimidyl glutarate (DSG), disuccinimidyl suberate (DSS) and poly (ethylene glycol)-ylated bis (sulfosuccinimidyl)suberate (BS(PEG)5), were evaluated, and DSS at a concentration of 500 μM was confirmed to be optimal. The E. coli-cell-based regenerable HRP immunoassay was evaluated employing three sequential HRP treatment and regeneration steps. Then, the Oms of E. coli cells were isolated and layered on a microplate and regenerable OM-based HRP immunoassaying was evaluated. Five HRP immunoassays with four regeneration steps were found to be feasible. This regenerable, covalently immobilized, orientation-controlled OM-based immunoaffinity layer was applied to an SPR biosensor, which was capable of quantifying C-reactive protein (CRP). Five regeneration cycles were repeated using the demonstrated immunoaffinity layer with a signal difference of <10%. 相似文献
67.
Sun Woo Jin Gi Ho Lee Ji Yeon Kim Chae Yeon Kim Young Moo Choo Whajung Cho Eun Hee Han Yong Pil Hwang Yong An Kim Hye Gwang Jeong 《International journal of molecular sciences》2022,23(3)
Skeletal muscle is a heterogeneous tissue composed of a variety of functionally different fiber types. Slow-twitch type I muscle fibers are rich with mitochondria, and mitochondrial biogenesis promotes a shift towards more slow fibers. Leucine, a branched-chain amino acid (BCAA), regulates slow-twitch muscle fiber expression and mitochondrial function. The BCAA content is increased in porcine whole-blood protein hydrolysates (PWBPH) but the effect of PWBPH on muscle fiber type conversion is unknown. Supplementation with PWBPH (250 and 500 mg/kg for 5 weeks) increased time to exhaustion in the forced swimming test and the mass of the quadriceps femoris muscle but decreased the levels of blood markers of exercise-induced fatigue. PWBPH also promoted fast-twitch to slow-twitch muscle fiber conversion, elevated the levels of mitochondrial biogenesis markers (SIRT1, p-AMPK, PGC-1α, NRF1 and TFAM) and increased succinate dehydrogenase and malate dehydrogenase activities in ICR mice. Similarly, PWBPH induced markers of slow-twitch muscle fibers and mitochondrial biogenesis in C2C12 myotubes. Moreover, AMPK and SIRT1 inhibition blocked the PWBPH-induced muscle fiber type conversion in C2C12 myotubes. These results indicate that PWBPH enhances exercise performance by promoting slow-twitch muscle fiber expression and mitochondrial function via the AMPK/SIRT1 signaling pathway. 相似文献
68.
Effect of Amine Additive for the Synthesis of Cadmium Selenide Quantum Dots in a Microreactor
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Da‐Woon Jeong Basudev Swain Tae‐Yeon Seong Kyoung‐Tae Park Chan Gi Lee Bum Sung Kim 《International Journal of Applied Ceramic Technology》2016,13(2):223-227
The effect of octylamine flow rate on the structure and morphology of CdSe quantum dots synthesized in a microreactor was studied. The flow rate of octylamine was varied from 0.005 ml/min to 0.030 ml/min, and the optical properties of the synthesized particles were analyzed by UV–vis and photoluminescence spectroscopy. The particle size of the quantum dots was found to increase with an increasing octylamine flow rate. Further, UV–vis and photoluminescence bands were found to be red‐shifted with an increasing flow rate. We determined that, by controlling octylamine flow rate, the particle size of the quantum dots could be controlled. This method will help to determine the optimal octylamine flow conditions for synthesizing nanoparticles for use in a diverse range of applications. 相似文献
69.
Kyeong-Ho Yeon Heesu Park Seung-Hak Lee Yong-Min Park Sang-Hyup Lee Masakazu Iwamoto 《Korean Journal of Chemical Engineering》2008,25(5):1040-1046
Eutrophication caused by the excessive supply of phosphate to water bodies has been considered as one of the most important
environmental problems. In this study, the powder of zirconium mesostructure (ZM), which was prepared with the template of
surfactant, was immobilized in calcium alginate for practical application and the resulting material was tested to evaluate
the phosphate removal efficiency. Sorption isotherms and kinetic parameters were obtained by using the entrapped ZM beads
with 30 to 60% of ZM. The maximum sorption capacity increased with the higher ZM content. Q
max
in Langmuir isotherm was 51.74 mg/g for 60% of ZM with 7 mm of size. The smaller the particle size of the ZM beads, the faster
the rate of phosphate removal, because the phosphate ions had less distance to reach the internal pores of the immobilized
ZM beads. Chemical and electrochemical regeneration techniques were compared. Phosphates adsorbed on the ZM beads were effectively
desorbed with NaCl, NaOH, and Na2SO4 solutions. An electrochemical regeneration system consisting of an anion exchange membrane between two platinum-coated titanium
electrodes was successfully used to desorb and regenerate the phosphate-saturated ZM beads. Complete regeneration was reached
under optimal experimental conditions. Chemical and electrochemical regeneration proved the reusability of the bead form of
the entrapped ZM, and will enhance the economical performance of the phosphate treatment process. 相似文献
70.
Bo-Hyuk Jang Tae-Hun Yeon Hyun-Sik Han Yong-Ki Park Jae-Eui Yie 《Catalysis Letters》2001,77(1-3):21-28
Barium-containing NO
x
storage catalyst showed serious deactivation under thermal exposure at high temperatures. To elucidate the thermal deterioration of the NO
x
storage catalyst, four types of model catalyst, Pt/Al2O3, Ba/Al2O3, Pt–Ba/Al2O3, and a physical mixture of Pt/Al2O3 + Ba/Al2O3 were prepared and their physicochemical properties such as BET, NO TPD, TGA/DSC, XRD, and XPS were evaluated while the thermal aging temperature was increased from 550 to 1050°C. The fresh Pt–Ba/Al2O3 showed a sorption capacity of 3.35 wt%/g-cat. but the aged one revealed a reduced capacity of 2.28 wt%/g-cat. corresponding to 68% of the fresh one. It was found that this reduced sorption capacity was directly related to the deterioration of the NO
x
storage catalyst by thermal aging. The Ba on Ba/Al2O3 and Pt–Ba/Al2O3 catalysts began to interact with alumina to form Ba–Al solid alloy above 600°C and then transformed into stable BaAl2O4 having a spinel structure. However, no phase transition was observed in the Pt/Al2O3 catalyst having no barium component, even after aging at 1050°C. 相似文献