Bead-based polymerase chain reaction on a microchip

We present a bead-based approach to microfluidic polymerase chain reaction (PCR), enabling fluorescent detection and sample conditioning in a single microchamber. Bead-based PCR, while not extensively investigated in microchip format, has been used in a variety of bioanalytical applications in recent years. We leverage the ability of bead-based PCR to accumulate fluorescent labels following DNA amplification to explore a novel DNA detection scheme on a microchip. The microchip uses an integrated microheater and temperature sensor for rapid control of thermal cycling temperatures, while the sample is held in a microchamber fabricated from (poly)dimethylsiloxane and coated with Parylene. The effects of key bead-based PCR parameters, including annealing temperature and concentration of microbeads in the reaction mixture, are studied to achieve optimized device sensitivity and detection time. The device is capable of detecting a synthetically prepared section of the Bordetella pertussis genome in as few as 10 temperature cycles with times as short as 15?min. We then demonstrate the use of the procedure in an integrated device; capturing, amplifying, detecting, and purifying template DNA in a single microfluidic chamber. These results show that this method is an effective method of DNA detection which is easily integrated in a microfluidic device to perform additional steps such as sample pre-conditioning.     

基于Cortex-M3的多功能楼宇控制系统网关节点设计

针对目前大多公司的楼宇日常办公耗电无法做到智能节能控制,提出了一种基于多功能网关节点的集中控制解决方案。设计中提出利用基于Cortex-M3的LPC1766作为网关节点,并且集成DHT11温湿度传感器,光线传感器和继电器控制电路等。通过RS485和终端节点通讯并且通过以太网与上位机通讯.最终实现远程楼宇节能控制的三层体系解决方案。     

基于JSP的移动开发技术论坛的设计与实现

本文讨论基于B/S模式的一种JSP论坛的设计与实现。完成的功能模块包括用户注册,浏览板块,发帖,回帖等,管理员编辑,删除等管理帖子功能,管理管理员用户功能等。     

LFM信号限幅失真对侧扫声纳声图的影响

本文分情况讨论了LFM信号限幅失真对脉冲压缩及侧扫声图的影响并进行了湖试验证。结果表明:限幅失真对于脉压波形的主瓣几乎没有影响,即不会影响到声图的分辨率,但是会使脉压波形的幅度产生失真。当限幅程度较大时,由于信号的叠加会进一步恶化脉压波形,使脉压信号的幅度明显降低,甚至产生假目标,信号的动态变化范围被严重压缩;即使当单个信号不限幅时,而由于多目标回波相互叠加也可能会引起信号限幅,此时也可能对处理结果造成较为严重的失真,使信号动态范围被压缩。     

液力忆惯容器装置建模与特性试验

针对液力忆惯容器装置已有数学模型忽略因素过多的问题,考虑油液的惯性、动力学黏度以及装置内部的干摩擦,建立符合工程实际的非线性数学模型. 试制液力忆惯容器装置样机,并进行台架试验. 结果表明,当位移激励的频率较低时,液力忆惯容器装置提供的惯性力、黏滞阻尼力较小,可以近似等效为干摩擦阻尼器;当激励的频率较高时,装置等效为理想忆惯容器、非线性黏滞阻尼器、干摩擦阻尼器三元件并联. 装置的输出合力、惯性力、阻尼力试验值与仿真值最大偏差分别为5.70%、9.15%、5.47%,惯质特性曲线和寄生阻尼特性曲线的试验值与仿真值最大偏差分别为4.23%和8.50%,表明数学模型准确可靠,能够满足工程需要,为液力忆惯容器装置在减振系统中的应用奠定了基础.     

PARAMAGNETIC MEISSNER EFFECTS IN MELT-TEXTURED Y_(0.99)Nd_(0.01)Ba_2Cu_3O_(7-y)SUPERCONDUCTOR

The paramagnetic Meissner effect (PME) was observed on the field-cooled M-T curvesof melt-textured Y0.99Nd0.01Ba2Cu3O7-y in applied magnetic fields from 1kOe toJ0kOe. In the magnetic field below 1kOe, the sample undergoes the typical transi-tion from the normal state to the superconducting state when it was cooled down. Theapplied magnetic fields in which the PME occurs, are very high compared with thefields reported in literatures. We propose that the occurrence of the PME may be dueto a phase transition of flux vortices in the superconductors.     

焊接热源有效利用率的测试计算法

给出实际热输入值是正确求解焊接热弹塑性问题和焊接残余应力与变形问题的前提;因而,准确地确定热源有效利用率η,在焊接力学的发展中是一个急待解决的命题。本文推荐采用的“测试计算法”提供了一个能够准确、简捷地求得η值的新途径。引入被焊材料的熔化潜热系数后,给出了线热源加热焊缝金属至熔化温度(处于液态)时的实际热有效利用率ηt 的表达式。在四种不同类型的薄板材料上进行了钨极氩弧焊试验,测试计算结果与理论值相符合。理论推导与试验结果均证明,形成焊缝所需要的线能量的无量纲数值等于焊缝金属熔化热有效利用率ηt 的倒数。文中给出的焊接规范参数选择原则也适用于符合线热源条件的其它熔焊热源。η值不但与焊接工艺方法、规范参数有关,而且也随被焊材料的热物理特性不同而异。     

The Role of Chronic Inflammation in Various Diseases and Anti-inflammatory Therapies Containing Natural Products

Chronic inflammation represents a long-term reaction of the body's immune system to noxious stimuli. Such a sustained inflammatory response sometimes results in lasting damage to healthy tissues and organs. In fact, chronic inflammation is implicated in the development and progression of various diseases, including cardiovascular diseases, respiratory diseases, metabolic diseases, neurodegenerative diseases, and even cancers. Targeting nonresolving inflammation thus provides new opportunities for treating relevant diseases. In this review, we will go over several chronic inflammation-associated diseases first with emphasis on the role of inflammation in their pathogenesis. Then, we will summarize a number of natural products that exhibit therapeutic effects against those diseases by acting on different markers in the inflammatory response. We envision that natural products will remain a rich resource for the discovery of new drugs treating diseases associated with chronic inflammation.     

基于分段式虚拟阻抗的改进下垂控制

当线路电阻在线路阻抗中的影响不能忽略时,系统的有功功率和无功功率会发生耦合,会降低下垂控制的性能和系统无功功率的分配精度,为此本文提出了基于分段虚拟阻抗的改进下垂控制策略.以无功功率比值为依据,分段设计自适应线路虚拟阻抗,减小线路阻抗差异,增大无功功率分配精度;在无功功率-电压下垂控制中加入自适应电压补偿环节以补偿电压跌落;最后,通过仿真和实验验证自适应分段虚拟阻抗和电压补偿环节的有效性.     

Effects of Edible Insect Tenebrio molitor Larva Fermentation Extract as a Substitute Protein on Hepatosteatogenesis and Proteomic Changes in Obese Mice Induced by High-Fat Diet

Mealworms (Tenebrio molitor larva) are an edible insect and a protein-rich food; however, research on mealworms as a substitute protein is insufficient. In this study, mealworm fermentation extract (TMP) was assessed as a replacement for soy protein (SP) in a control diet (CON) or a high-fat diet (HFD) of mice for 12 weeks. TMP substitution reduced body weight, body weight gain, body fat mass (perirenal and mesenteric), fat size, glucose intolerance, and insulin resistance compared to the HFD-SP group. TMP alleviated hepatic steatosis (lipid contents and lipid droplets) in high-fat-fed mice and down-regulated the PPARγ, CD36, and DGAT2 gene levels. Proteomic analysis showed that a HFD for 12 weeks up-regulated 20 proteins and down-regulated 17 proteins in mice fed SP. On the other hand, TMP reversed the protein profiles. TMP significantly down-regulated KHK, GLO1, ATP5H, SOD, and DDAH1 and up-regulated DLD, Mup1, CPS1, Ces3b, PDI, and HYOU1 compared to the HFD-SP group. These proteins are involved in the glucose, lipid, and amino acid metabolism, as well as in oxidative stress and endoplasmic reticulum stress. Thus, substituting SP for TMP helped improve HFD-induced obesity, steatosis, and insulin resistance in mice. These results suggest that TMP is a potential substitute for commonly used protein sources.