Proteins interacting with CFTR and its mutants have been intensively studied using different experimental approaches. These studies provided information on the cellular processes leading to proper protein folding, routing to the plasma membrane, recycling, activation and degradation. Recently, new approaches have been developed based on the proximity labeling of protein partners or proteins in close vicinity and their subsequent identification by mass spectrometry. In this study, we evaluated TurboID- and APEX2-based proximity labeling of WT CFTR and compared the obtained data to those reported in databases. The CFTR-WT interactome was then compared to that of two CFTR (G551D and W1282X) mutants and the structurally unrelated potassium channel KCNK3. The two proximity labeling approaches identified both known and additional CFTR protein partners, including multiple SLC transporters. Proximity labeling approaches provided a more comprehensive picture of the CFTR interactome and improved our knowledge of the CFTR environment. 相似文献
This work is dedicated to the characterization by Atomic Force Microscopy (AFM) of Pseudomonas fluorescens, bacteria having high potential in biotechnology. They were first studied first in optimal conditions in terms of culture medium and temperature. AFM revealed a more-or-less elongated morphology with typical dimensions in the micrometer range, and an organization of the outer membrane characterized by the presence of long and randomly distributed ripples, which are likely related to the organization of lipopolysaccharides (LPS). The outer membrane also presents invaginations, some of them showing a reorganization of ripples, which could be the first sign of a bacterial stress response. In a second step, bacteria grown under unfavorable conditions were characterized. The choice of the medium appeared to be more critical in the case of the second generation of cells, the less adapted medium inducing not only changes in the membrane organization but also larger damages in bacteria. An increased growth temperature affected both the usual “swollen” morphology and the organization of the outer membrane. Here also, LPS likely contribute to membrane remodelling, which makes them potential markers to track cell state changes. 相似文献
Journal of Inorganic and Organometallic Polymers and Materials - In the present study, a modified solvothermal reaction of (hematite) with titanium(IV) butoxide and gold(III) chloride produced... 相似文献
The main purpose of this study is the use of a Tunisian bio resource for the improvement of mechanical and physical properties of braided polyethylene terephthalate (PET) sutures. In this study, antibacterial PET sutures were developed using Tunisian essential oil of Laurus nobilis. A new lubrication process was proposed for this fact. The essential oil of L. nobilis was selected because of its antibacterial properties as well as analgesic, antiseptic, antiviral, and anti-infectious proprieties. Optimized extraction process of essential oil was also determined. Characteristics and cytotoxicity of extracted essential oil were also investigated. In order to study the impact of lubrication conditions on PET suture performances, mechanical properties and antibacterial effects of braided sutures were studied. The obtained results prove that sutures lubrication improves suture knot efficiency and leads to smoother surface. Developed sutures show also obvious antibacterial effect against Staphylococcus aureus ATCC 6538 G (+) colonies. 相似文献
Digital technologies are becoming present and essential in all sectors of our lives. In education, the intensive usage of digital learning devices contributes to generating a large amount of trace data from digital learning activities. Intelligent exploitation of these traces represents a valuable asset for both device producers (to improve the design of the devices) and consumers (learners and teachers). In this paper, we first share our vision for better exploitation by teachers, of traces from middle schoolers' digital activities generated by their use of tools and digital learning services during different classes. This vision is a part of the AT41 project funded by the French Ministry of Education. This exploitation has to meet the requirements of the different teachers. Conducting such a project is not an easy task, because it has to consider the following issues: the lack of comprehensive and clear methodology to design and exploit these traces; ②heterogeneity of teacher requirements that complicates their elicitation and analysis; ③the diversity of trace sources. Secondly, we propose a requirement-driven architecture for Learning Analytics composed of a well-identified life cycle. This architecture is augmented by learner traces. It offers a repository storing both teacher requirements and traces to facilitate the Learning Analytics in generating relevant and valuable indicators. 相似文献
In the present study, ternary Au-ZnO/rGO nanocomposite was prepared using a modified polyol protocol. The ternary structure was attained by deposition of both gold nanoparticles (AuNPs) and ZnO NPs on the rGO surface. No surfactants or ligands are used in this chemical process. On the other hand, 1,3-propanediol was used as solvent, reducing agent and surfactant to ensure the formation of NPs and inhibit particles accumulation. The XRD data confirm the successful formation of the three materials and the high crystallinity of the as-prepared sample. Moreover, the XPS measurements confirmed the high purity of the nanocomposite. TEM images show the formation of ternary Au/ZnO/rGO nanostructure. However, Au and ZnO NPs exhibited spherical shape with an average size of 20 nm and homogeneously distribution onto the rGO surface. The ternary Au-ZnO/rGO nanocomposite exhibited optical response in both UV and visible region due to the plasmonic properties of AuNPs. The BET data revealed an increase of the surface area of Au-ZnO/rGO nanocomposite compared to bare ZnO and hybrid Au-ZnO NPs which render it a promising system for high photocatalytic activity. The preliminary photodegradation measurements against MB molecules prove the high performance of the ternary Au-ZnO/rGO nanocomposite to decompose pollutant molecules compared to bare ZnO. The observed photocatalytic activity enhancement could be attributed to the apport given by both plasmonic properties of AuNPs and the high surface area of rGO.
Autonomic neuropathy in diabetes mellitus can cause alterations of the motor function of various segments of the gastroenteric apparatus. With hepatocholangio-cholecystiscintigraphy-HIDA we have studied the motility of the biliary system in patients with diabetes mellitus type II. The research has been carried out in 29 patients with diabetic autonomic neuropathy; 12 healthy volunteers have been studied to compare the results obtained. The results showed a delay in the appearance of radionucleotide in the small intestine of diabetic subjects compared to controls with statistical significance. Moreover the diabetic subjects with a serious neuropathic injury showed increased intestinal transit time. These results match those obtained by other authors that have studied the cholecystic emptying in diabetic subjects with other methods. Consequently the biliary system is also affected by the diabetic autonomic neuropathy that can be in its turn the cause of other pathologies such as biliary lithiasis. 相似文献