Activation of PKCalpha downstream from caspases during apoptosis induced by 7-hydroxystaurosporine or the topoisomerase inhibitors, camptothecin and etoposide, in human myeloid leukemia HL60 cells

We previously demonstrated that the anticancer agent and protein kinase C (PKC) inhibitor 7-hydroxystaurosporine (UCN-01) induces apoptosis independently of p53 and protein synthesis in HL60 cells. We now report the associated changes of PKC isoforms. PKCalpha, betaI, betaII, delta, and zeta activities were measured after immunoprecipitation of cytosols from UCN-01-treated HL60 cells. UCN-01 had no effect on PKCzeta and inhibited kinase activity of PKCbetaI, betaII, and delta. PKCalpha activity was initially inhibited at 1 h, and subsequently increased as cells underwent apoptosis 3 h after the beginning of UCN-01 treatment. Camptothecin (CPT) and etoposide (VP-16) also markedly enhanced PKCalpha activity during apoptosis in HL60 cells. However, CPT did not affect PKCbetaI, betaII and zeta, and activated PKCdelta. PKCalpha activation was not due to increased protein levels or proteolytic cleavage but was associated with PKCalpha autophosphorylation in vitro and increased phosphorylation in vivo. We also found that not only PKC delta but also PKC betaI was proteolytically activated in HL60 cells during apoptosis. The PKCalpha activation and hyperphosphorylation were abrogated by N-benzyloxycarbonyl-Val-Ala-Asp(O-methyl)-fluoromethylketone (z-VAD-fmk) under conditions that abrogated apoptosis. z-VAD-fmk also prevented PKCdelta and betaI proteolytic activation. Together these findings suggest that caspases regulate PKC activity during apoptosis in HL60 cells. At least two modes of activation were observed: hyperphosphorylation for PKCalpha and proteolytic activation for PKC delta and betaI.     

加强无线电管理,日常监督检查势在必行

随着社会经济的迅速发展,无线电通信事业取得了很大进步,无线电通信广泛应用于各行各业,在经济发展、社会进步中发挥了重要的作用.但目前一些单位和个人无视国家有关频率管理和无线电设备管理的有关法规,擅自占用频率和非法设台情况比较严重,极大地损害了电磁环境和秩序,干扰了航空导航、公安、消防、护林防火、“三防”等重要通信业务,对无线电通信的可持续性发展造成了极大隐患,加强无线电管理日常监督检查势在必行.     

EDA介质快慢横波时差和振幅-方位曲线的实验分析

在含垂直定向裂隙的各向异性（EDA）介质中，地震快、慢横波传播的时差及振幅随裂隙（缝）方位变化曲线能反映裂缝的密度、走向和介质的性质，这对多波地震资料的处理、解释有着重要意义。但受观测条件限制以及噪声的影响，在横波分裂记录上，同相轴往往会出现扭曲、倾斜和不规则抖动等现象，给快、慢横波时差的求取和振幅-方位曲线的绘制带来误差。设计了EDA介质物理模型，并利用岩石超声波测量系统获得了快、慢横波记录；分析记录可知，在相同传播方向的分裂横波具有走时和传播速度不随裂缝方位变化的恒定性特征；以此为基础，提出了利用平均法和瞬时振幅法来计算快、慢横波的走时时差和绘制振幅-方位曲线，以提高精度。利用物理模型实验得到的2个正交横波（S1波与S2波）的快、慢横波记录，分别用平均法和瞬时振幅法计算了快、慢横波走时的平均值、时差和均方差，并绘制了相应的振幅一方位曲线。结果表明，采用上述2种方法，消除了同相轴扭曲、倾斜和不规则抖动等对计算结果的影响，时差的计算是可靠的，振幅一方位曲线的绘制是合理的。     

Instability due to Viscosity Stratification Downstream of a Centerline Injector

A common injector geometry upstream of a static mixer is the centerline injector. A flow instability can arise due to viscosity differences between the injected core‐flow and the outer co‐flow. This instability can adversely affect the effectiveness of the mixing operation. An experimental investigation of miscible viscosity‐stratified flow in a circular geometry was performed using Laser Induced Fluorescence (LIF) and Particle Image Velocimetry (PIV). The experimental results for the stable region agree with the analytical results. The unstable region exhibits different modes depending on the viscosity ratio, volume flux ratio, and Reynolds number. The modes include wavy core‐flow with fissures and wavy core‐flow with core breakup. The time‐averaged experiment velocity profiles for the unstable core indicate a broadening of the jet at the centerline, which is consistent with the LIF visualization.     

一种基于尼古拉兹曲线确定管段阻力系数的新方法

文章提出了一种以尼古拉兹曲线为原理来确定注水系统中各个管段阻力系数λ的新方法.以水力实验为基础,选取具有代表性的管段进行实验,对实验数据分析、验证,并对现场测试管线进行数据拟合.拟合数据表明此方法可行、有效.     

成形工艺对快速凝固Al—Cu—Mg合金组织和性能的影响

采用自制离心雾化装置制取了快速凝固LY12硬铝合金粉末,研究了以该粉末制成的真空热压件和挤压丝材的组织和性能。结果表明,所得粉末为泪滴状,其显微组织为树枝状晶和胞状晶。真空热压件密度高时所对应的强度高。450℃、10min、150MPa为较合理的工艺,此时粉末颗粒间结合密实,晶粒尺寸没有明显改变。在500℃热挤压时,挤压丝的组织已发生了再结晶,晶粒尺寸仍较细小。挤压丝材的抗拉强度达460MPa,其断口为韧窝状,韧窝尺寸小于3μm,且分布均匀。     

Fluorescence spectra of ARE2I5 and AREI3 in the solid state (A ≡ K, Rb, Cs, Ti; RE ≡ Sm, Eu)

The fluorescence spectra of ARE₂I₅(s) and AREI₃(s) (A ≡ K, Rb, Cs, Tl; RE ≡ Sm₁ Eu) have been studied systematically. The regular shift of the central wavelengths is discussed in a preliminary manner on the basis of crystal lattice field and covalence effects.     

27keV Ar~+斜入射时的Cu-Au合金溅射实验角分布

用27keV Ar~+分别在垂直和倾斜入射情况下,轰击了Cu-Au(30wt％)合金样品,测量了Cu和Au原子的溅射角分布。角分布是用卢瑟福背散射(RBS)技术分析Al捕获膜上的Cu-Au沉积成分而定量得到的。结果表明:(1)在Ar~+倾斜(θ=40°)入射时,Cu原子择优发射,且程度比垂直入射(θ_r=O°)时增强:(2)倾斜入射时,Cu原子的角分布显示出在接近于样品表面法线方向的发射角范围内(θ<45°),发射机率比垂直入射时减小。