Caveolin-1 Alleviates Acetaminophen—Induced Hepatotoxicity in Alcoholic Fatty Liver Disease by Regulating the Ang II/EGFR/ERK Axis

Acetaminophen (APAP) is a widely used antipyretic analgesic which can lead to acute liver failure after overdoses. Chronic alcoholic fatty liver disease (AFLD) appears to enhance the risk and severity of APAP-induced liver injury, and the level of angiotensin II (Ang II) increased sharply at the same time. However, the underlying mechanisms remain unclear. Caveolin-1 (CAV1) has been proven to have a protective effect on AFLD. This study aimed to examine whether CAV1 can protect the APAP-induced hepatotoxicity of AFLD by affecting Ang II or its related targets. In vivo, the AFLD model was established according to the chronic-plus-binge ethanol model. Liver injury and hepatic lipid accumulation level were determined. The levels of Angiotensin converting enzyme 2 (ACE2), Ang II, CAV1, and other relevant proteins were evaluated by western blotting. In vitro, L02 cells were treated with alcohol and oleic acid mixture and APAP. CAV1 and ACE2 expression was downregulated in APAP-treated AFLD mice compared to APAP-treated mice. The overexpression of CAV1 in mice and L02 cells alleviated APAP-induced hepatotoxicity in AFLD and downregulated Ang II, p-EGFR/EGFR and P-ERK/ERK expression. Immunofluorescence experiments revealed interactions between CAV1, Ang II, and EGFR. The application of losartan (an Ang II receptor antagonist) and PD98059 (an ERK1/2 inhibitor) alleviated APAP-induced hepatotoxicity in AFLD. In conclusion, our findings verified that CAV1 alleviates APAP-aggravated hepatotoxicity in AFLD by downregulating the Ang II /EGFR/ERK axis, which could be a novel therapeutic target for its prevention or treatment.     

不同尺寸段塞组合等流度二元驱驱油效果评价

辽河油田锦16块实施二元复合驱以来,仍存在部分注入井单层吸液量过大,使注入液在各层中的推进速度不一致,从而导致相应的采油井聚合物质量浓度上升较快的问题。通过室内驱替实验,采用均质岩心和非均质岩心,对比不同段塞组合的等流度二元驱驱油方法在非均质岩心上的驱油效果。结果表明,在相同聚合物用量和总注入孔隙体积倍数条件下,在三层非均质岩心上等流度驱油方法比常规单段塞驱油方法化学驱采出程度高出3.54%;多段塞等流度驱油方法可以明显提高中、低渗层分流率,降低高渗层分流率。因此,合理段塞组合等流度二元驱油方法可有效提高高渗透非均质油藏的采收率。     

Evaluation of apatite-coated chitosan microspheres for bone regeneration

The apatite-coated chitosan microspheres were fabricated by incubating chitosan in five times simulated body fluid. The apatite deposited on the microspheres was similar to natural bone mineral, as demonstrated by scanning electron microscopy, X-ray diffraction spectra and Fourier transformed-infrared spectroscopy analyses. Rat bone marrow-derived mesenchymal stem cells （BMSCs） were seeded on apatite- coated microspheres to investigate the effect of this scaffold on cell proliferation and differentiation. BMSCs seeded on uncoated microspheres were served as a control. In vivo experiment was evaluated by transplanting the microspheres loaded with or without BMSCs in 5-mm cranial defects of Wistar rats. Bone regeneration was investigated via micro-CT and histological analysis. It was found that apatite-coated chitosan microspheres could significantly promote the proliferation and alkaline phosphatase activity of BMSCs compared with uncoated microspheres. Histological slices and Micro-CT images at 8 weeks revealed much better regeneration of bone in the apatite-coated microspheres loaded with BMSCs than the control. In addition, the defect filled with pure microspheres induced little new bone formation. Our findings suggest that the apatite-coated chitosan microspheres scaffold is a promising carrier of stem cells for cranial bone tissue engineering.     

Microstructural evolution and mechanism of grain growth in magnesia ceramics prepared by high pressure and temperature with ultra-high heating rate

The fast densification method of combustion reaction plus quick pressing was adopted to prepare nanocrystalline ceramics.The densification process of magnesia compact with a particle size of 100 nm was investigated,under the applied pressure of up to 170 MPa,and the temperature range of 1740–2080 K with ultra-high heating rate(above 1700 K/min).High-purity magnesia ceramics with a relative density of 98.8%and an average grain size of 120 nm was obtained at 1740 K,and the grain growth during the densification process was effectively restrained.The characteristic morphology of evaporation-condensation was observed in the compact prepared at 2080 K,which revealed the actual process of mass transfer by gas diffusion.Moreover,the investigation on the microstructure evolution and mechanism of grain growth was carried out,on the basis of as-preserved nanocrystalline ceramics.The result indicated that the grain growth of the nanocrystalline MgO was controlled by the mechanism of evaporation-condensation rather than surface diffusion.Furthermore,the pressure had an influence of restraining the grain growth based on solid diffusion and strengthening the effect of gas diffusion with the increasing temperature.Under the particular conditions,there existed an appropriate temperature for the densification of nanocrystalline magnesia,while the excessive temperature would exaggerate grain growth and impede densification.     

齿上开槽对滤波减速器力学性能的影响

本文使用有限元法研究滤波减速器轮齿上开槽对滤波减速器轮齿的动力学性能和传动性能的影响。通过对滤波减速器齿上不同开槽形状进行有限元对比分析,结果显示直槽对轮齿力学性能有很好的改善作用。在此基础上分析了直槽的槽深、槽宽、应力释放孔大小和应力释放孔与直槽的倒角大小对轮齿力学性能的影响以及不同载荷下轮齿应力分布情况。研究结果表明在滤波减速器齿上开直槽,当槽深大于轮齿模数的三倍,应力释放孔和倒角的半径大于槽宽的十倍,槽缝宽度不超过轮齿模数的十分之一时,其接触应力、弯曲应力和冲击振动较小。     

Stochastic formulation of particle kinetics in wall-bounded two-phase flows

This paper presents a generalized framework of stochastic modeling for particle kinetics in wall-bounded flow. We modified a reflected Brownian motion process and straightforwardly obtained a Kramers equation for particle probability density function (PDF). After the wall effects were accounted for as a drift from zero in the mean displacement and suppression in the diffusivity of a particle, an analytical solution was worked out for PDF. Three distinguishable mechanisms were identified to affect the profile of particle probability distribution: external forces, turbophoresis effect, and wall-drift effect. The proposed formulation covers the Huang et al. (2009) model of a wall that produces electrostatic repulsion force and van der Waals force, as well as Monte-Carlo solutions for the Peter and Barenbrug (2002) model under a variety of relaxation times. Moreover, it successfully reproduces the two patterns of particle concentration profiles observed in experiments of sediment-laden open-channel flows. The strength of the wall-drift effect was found to be connected with the interaction frequency between particle and wall. Further exploration of the relationship among flow turbulence, particle inertia, and particle concentration is worthwhile.     

铝质易拉罐轻量化实现途径及其关键技术

轻量化是降低铝质易拉罐生产成本、促进技术进步的重要手段。分析了实现铝质易拉罐轻量化的主要技术途径,包括减薄铝板材厚度和减小罐口直径2个方面。在此基础上,探讨了改善铝板材冶金质量与变形性能、优化罐型并改进工装模具等实现轻量化的关键技术。     

活性炭纤维对凝结水中 Fe3＋的吸附研究

通过静态吸附和动态吸附实验,考察了活性炭纤维（ ACF）对Fe3＋的吸附性能,研究了不同实验条件下的动态吸附穿透曲线;通过扫描电镜,观察了吸附前后ACF的表面形貌。结果表明：ACF对Fe3＋的吸附既有物理吸附又有化学吸附;随着ACF比表面积增大或吸附溶液温度升高,对Fe3＋的吸附容量增大;动态吸附时,当流速为10 mL／min,最有利于出水达标。     

地铁专用间接蒸发冷却器布水方式优化

为了解决常规间接蒸发冷却器由于表面水膜均匀性、完整性差而导致换热效率低的问题,提出了两侧旋转布水间接蒸发冷却器,进行了3种布置方式下的换热性能实验,运用正交实验对影响换热器性能的因素进行研究,研究表明:开孔正对气流方向时换热器换热性能最佳,且旋转布水装置存在最佳转速76 r/min,喷水量、空气流速、冷却水流量、冷却水进口温度的增加使换热器的换热量增大,喷水温度、空气温度的升高使换热器的换热量减少,其中冷却水进口温度的改变对换热性能的影响最为显著,温度由35℃上升到39℃时,换热量提高37.62％,单位面积换热量为1.14 kW,该换热器可安装于地下车站排风坑道内,可有效地解决地铁站冷却塔安装位置难题.     

浅谈子程序在数控铣教学中的应用

子程序是数控编程过程中的一种常用功能,灵活使用子程序技术,可以减少编程时的程序量,增强数控机床的功能。本文针对子程序调用技术在数控铣床教学中的几种常用方法进行探讨与研究,希望对同行起一个抛砖引玉的作用。