Transcellular retrograde labeling of radial glial cells with WGA-HRP and DiI in neonatal rat and hamster

Topographically distinct populations of radial glial cells in the diencephalon and mesencephalon of neonatal rats and hamsters were transcellularly labeled with wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) and with the lipophilic tracer DiI. A comparison of the histological distribution of the two tracers is suggestive of two different mechanisms of transcellular labeling. Intraocular injections of WGA-HRP resulted in the uptake of exogenously applied WGA-HRP by retinal ganglion cells, followed by anterograde axonal transport and exocytosis within the optic target nuclei. In addition to the transneuronal labeling, which is typical of such injections, we observed the transcellular labeling of the processes and somata of radial glial cells that were topographically associated with the terminal fields of the labeled axons. Similar transcellular labeling of radial glial cells associated with the axon terminal fields of the colliculogeniculate projection to the medial geniculate nucleus was observed following injections of WGA-HRP in the inferior colliculus. The transcellular labeling within the radial glial cells was discontinuous and somatopetally concentrated, indicating the existence of a retrograde active transport mechanism within the radial glial processes subsequent to its uptake following release of tracer from axons. This type of labeling can be referred to as transcellular retrograde glioplasmic transport. In contrast, DiI was used as a tracer through its capacity to diffuse within the plasmalemma. Topographically distinct populations of radial glial cells were transcellularly labeled following placements of DiI in the retina, inferior colliculus, or dorsal thalamus of fixed brains. The radial processes of labeled radial glial cells consistently extended into regions that also contained labeled axons. It is likely that the transcellular radial glial labeling with DiI occurred via transmembranous diffusion. These data indicate that a close structural and functional relation exists between axons and glial cells in the developing brain.     

宽波段高灵敏快响应热释电型光卡计的研究

采用热释电材料,设计并研制了适用于红外-可见-X光波段的高灵敏、快响应光能量计。针算了卡计的参数,对1.06μm、0.53/μm激光和0.1～10keV的软X射线进行了能量测量,灵敏度达300mV/μJ,时间响应小于2ns。     

水溶液辐射分解产物对金属材料腐蚀的影响

实验研究了水辐射分解作用对高放废物深地质处置容器材料的影响。在高放废物深地质处置库附近的地下水,受辐射线作用后分解出氧、氢和氧化产物(例如H_2O_2等),于是在高放废物容器周围形成一个氧化场,导致高放废物中的某些重要放射性核素(例如U,Np和Tc)易溶于水,并向远域迁移。本实验中的含FeSO_4(0.13 mol/L)水溶液在吸收剂量为0、20、60、180、500kGy的射线作用下,其氧化还原电位(Eh)值由+357mV增至+414 mV;在不同吸收剂量的射线作用下,金属Cu、金属Al、0.357 mv金属Fe和不锈钢(后者是我国拟采用的高放废物包装容器材料),在水溶液中的氧化侵蚀强度,分别比在无辐射情况下的大0.2-6.1倍。     

硅灰混凝土的研究及其在公路道面的应用

 了提高道路路面混凝土的抗磨能力，采用硅灰代替部分水泥的方法，对硅灰混凝土的性能进行了较系统的试验研究。成果表明其抗压强度、抗弯强度、抗磨强度等指标都得到明显提高，全面达到和超过了规范要求。并已在丹江口水利枢纽上坝公路中得到应用，实践证明硅灰混凝土技术可靠、经济合理、易于施工。特别是抗磨度的提高，对延长公路路面的使用寿命具有重要意义。     

A monoclonal antibody selected for probing the folding of staphylococcal nuclease and its N-terminal fragments

PURPOSE: Nitric oxide (NO) is generated in mammalian tissue by the conversion of L-arginine to L-citrulline. The reaction is catalyzed by nitric oxide synthase (NOS). NO has been suggested to have a dual role in tumor biology with both antitumor and tumor promoter activity. Furthermore, it has been proposed that NO contributes to interleukin-2-induced antitumor activity. Since interleukin-2 is used in the treatment of renal cell carcinoma (RCC) it was of interest to study the NOS activity in the human kidney and in RCC and its correlation to tumor grade. Furthermore, the effect of cytokine treatment on NOS activity and the effect of NO donor application was studied in cultured cells. MATERIALS AND METHODS: The effect of cytokine treatment on NOS activity and the effect of NO donor application on cell proliferation was studied in cultured human proximal tubular cells and in RCC cell lines HN4 and HN51. NOS activity was measured by the L-arginine to L-citrulline conversion assay. RESULTS: Calcium-dependent NOS activity was found in all non-malignant kidney tissues (486+/-63 pmol. min(-1) g(-1) tissue). The activity was significantly lower in RCC (24+/-6 pmol. min(-1) g(-1) tissue) and correlated with tumor grade; thus high grade tumors showed lower activity than low grade tumors. Calcium-independent NOS activity was not detected in non-malignant kidney tissue or in RCC tissue. In cultured proximal tubular cells and RCC cell lines HN4 and HN51, cytokine treatment induced a marked increase in NOS activity and NO exerted cytostatic effects on these cell lines. Conclusions: The NOS activity was higher in non-malignant kidney tissue than in RCC tissue and was inversely correlated with tumor grade. Furthermore, cytokine treatment induced a marked increase in NOS activity and NO exerted cytostatic effects on cultured proximal tubular cells and RCC cell lines.     

非接触式半导体少子寿命测试方法——微波反射法

介绍了一种非接触式用于测量少子寿命的微波反射法，并与通常的光电导衰退法进行了比较。     

1，2─双（二乙基膦基）乙烷的合成

以二乙基氯化膦为原料，经乙炔化及加氢两步合成了１，２－双（二乙基膦基）乙烷，产率超过文献值。用红外光谱和核磁共振波谱分别测定了结构。     

波束扫描合成孔径雷达成像处理

本文主要研究了滤束扫描合成孔径雷达的杨像处理方法，分析了ＳＣＡＮＳＡＲ的工作原理、信号格式、信号结构和信号参数限制，成像处理算法包括块数据成像算法和块图像拼接算法两部分，频谱分析法和时域频域混合相关法被用于数据成像，块图像的拼接是利用时间有系剪裁块图像再顺次拼接，计算机仿真验证了本文提出的ＳＣＡＮＳＡＲ信号格式和成像算法的可行性。     

桌域网及其新技术的研究

桌域网是一种适合于多媒体业务需要的小型ATM网 ,是对传统计算机网络的体系结构的一种补充。本文介绍了设计桌域网的基本策略 ,讨论了它的设计目标和特性 ,给出了桌域网的体系结构、网络接入设备及其性能 ,最后提出研究桌域网的几点建议。