Immunostimulatory CpG oligonucleotides form defined three-dimensional structures: results from an NMR study

The DNA eicosamer 5'-TCCATGACGTTCCTGATGCT-3' is known to stimulate the innate immune system of vertebrae. The immunostimulatory activity is based on the activation of Toll-like receptor 9 (TLR9). While it is known that the CG dinucleotide of the eicosamer has to be unmethylated, the structural basis of the recognition of the DNA through the receptor remains unclear. Oligodeoxynucleotides containing the sequence of the eicosamer, or a portion thereof, ranging in length from hexamer to pentaeicosamer were studied by (1)H NMR spectroscopy. Based on two-dimensional NMR spectra, a number of resonances could be unambiguously assigned. For all oligonucleotides, structural transitions were detected upon heating, as monitored by the line width and chemical shift of low-field resonances. This includes the TC dinucleotide of the 5'-terminal portion, which does not have any clear base-pairing partners. The melting transitions, together with the NOESY cross-peaks, demonstrate that structure formation occurs well beyond the core hexamer 5'-GACGTT-3', a fact that may be important for understanding the molecular recognition by the Toll-like receptors of the innate immune system.     

Effect of vanadium and chromium on the microstructural features of V–Cr–Mn–Ni spheroidal carbide cast irons

The objective of this investigation is to study the influence of vanadium（5.0wt%–10.0wt%） and chromium（0–9.0wt%） on the microstructure and hardness of Cr-V-Mn-Ni white cast irons with spheroidal vanadium carbides. The alloys＇ microstructural features are presented and discussed with regard to the distribution of phase elements. The structural constituents of the alloys are spheroidal VC, proeutectoid cementite, ledeburite eutectic, rosette-shaped carbide eutectic（based on M7C3）, pearlite, martensite, and austenite. Their combinations and area fraction（AF） ratios are reported to be influenced by the alloys＇ chemical composition. Spheroidized VC particles are found to be sites for the nucleation of carbide eutectics. Cr and V are shown to substitute each other in the VC and M7C3 carbides, respectively. Chromium alloying leads to the formation of a eutectic（γ-Fe ＋ М7С3）, preventing the appearance of proeutectoid cementite in the structure. Vanadium and chromium are revealed to increase the total carbide fraction and the amount of austenite in the matrix. Cr is observed to play a key role in controlling the metallic matrix microstructure.     

云环境下基于本体的用户行为分析引擎研究

针对当前大型互联网站提升用户体验的需求,本研究创造性地提出基于本体的用户行为分析引擎的思想,它通过上下文感知技术,实时获取用户访问页面时的上下文信息(以下也称为动态行为),基于本体对用户行为建模,建立基于规则的上下文推理算法,对监测到的动态用户行为进行实时推理,及时反馈结果信息给用户。实验表明:本研究理论上独辟蹊径,设计实现上结合了云存储技术,能够一定程度改善用户体验效果。     

Simulation of clinker grinding circuits of cement plant based on process models calibrated using GA search method简

Particle size distributions of obtained samples from several sampling campaigns were determined and raw data were mass balanced before being used in simulation studies.After determination of breakage function,selection function,Bond work index,residence time distribution parameters,and Whiten＇s model parameters for air separators and diaphragms between the two compartments of tube ball mills,performance of the circuits was simulated for given throughputs and feed particle size distribution.Whiten＇s model parameters were determined by GA（genetic algorithm） toolbox of MATLAB software.Based on implemented models for modeling and simulation,optimization of circuits was carried out.It increased nearly 10.5% and 15.8% in fresh feed capacity input to each tube ball mill.In addition,circulating load ratios of circuits are modified to 118% and 127% from low level of 57% and 22%,respectively,and also cut points of air separators are adjusted at 30 and 40 μm from high range of 53 and 97 μm,respectively.All applications helped in well-operation and energy consumption reduction of equipments.     

A novel approach to prevent bubble coalescence during measurement of bubble size in flotation简

Effect of frothers in preventing bubble coalescence during flotation of minerals has long been investigated. To evaluate the performance of a frother, an apparatus to measure the bubble size is a basic necessity. McGill Bubble Size Analyzer （MBSA） or bubble viewer that has been developed and completed by McGill University＇s Mineral Processing Group during the last decade is a unique instrument to serve this purpose. Two parameters which are thought to influence the bubble size measurements by McGill bubble viewer include water quality and frother concentration in the chamber. Results show that there is no difference in Sauter mean （D32） when tap or de-ionized water was used instead of process water. However, the frother concentration, in this research DowFroth 250 （DF250）, inside the chamber exhibited a pronounced effect on bubble size. Frother concentration below a certain point can not prevent coalescence inside the chamber and therefore caution must be taken in plant applications. It was also noted that the frother concentration which has been so far practiced in plant measurements （CCC75-CCC95） is high enough to prevent coalescence with the bubble viewer.     

塑料绝缘电话线超高速生产流水线

一、概况最近,电话线挤塑流水线的最高速度已有显著提高。早在5～10年前1000～1200米/分的速度就很普遍了,而目前速度一般在2000～2500米/分,但因种种原因,实际生产速度仍然很低。由于速度较高,所以会导致张力、挤塑、冷却和收线控制困难,同时还降低了流水线的可靠性。为了提高实际生产速度,对上述因素进行研究,并尽力消除这些因素是非常重要的。     

介质损耗因数增量作为局部放电强度量度的探讨

通常认为用电桥测量tgδ隨电压的增量能够给出真实的放电强度量。有人已指出过,由于Δtgδ与放电脉冲的幅值和相位两者都有关系,所以,即或Δtgδ完全是由放电引起的,它也不能确切说明真实的放电强度。描述Δtgδ,ΔC与放电脉冲相位间关系的理论表达式的正确性已被实验结果以及关于高次谐波作用的计算所证实。本文给出C和tgδ测量值的简单函数关系式,它可用来改进西林电桥的测量结果。用这些函数可以分别估算放电强度和相位。列举出函数随电压变化的实际例子。最后讨论了Δtgδ对电桥电压谐泼畸变的敏感性。结果表明,电桥测量局部放电强度必须用无畸变的电桥电压。     

统计工程和应用统计学的正确结合

自从我们提出了统计工程的观点和理论后（译者注：本文作者先后在2010年5月、6月和12月的《质量进展》杂志上发表了3篇关于统计工程的文章,译文分别刊登于《中国认证认可》杂志2011年第1期、第2期和第8期）,     

Improved slope safety analysis by new Druker-Prager type criterion

Based on Mohr-Coulomb (M-C) criterion, the parameters of Druker-Prager (D-P) criterion for geomaterial were determined under non-associated flow rule, and thus a new D-P type criterion was presented. Two assumptions were employed during the derivation: 1) principal strains by M-C model and D-P model are equal, and 2) the material is under plane strain condition. Based on the analysis of the surface on π plane, it is found that the proposed D-P type criterion is better than the D-P criterion with M-C circumscribed circle or M-C inscribed circle, and is applicable for stress Lode angle less than zero. By comparing the predicted results with the test data of sand under plane strain condition and other D-P criteria, the proposed criterion is verified and agrees well with the test data, which is further proved to be better than other D-P type criteria in certain range of Lode angle. The criterion was compiled into a finite difference package FLAC3D by user-subroutine, and was used to analyze the stability of a slope by strength reduction method. The predicted slope safety factor from the proposed criterion agrees well with that by Spencer method, and it is more accurate than that from classic D-P criteria.