Ex vivo expansion with stem cell factor and interleukin-11 augments both short-term recovery posttransplant and the ability to serially transplant marrow

The characterization of many cytokines involved in the control of hematopoiesis has led to intense investigation into their potential use in ex vivo culture to expand progenitor numbers. We have established the optimum ex vivo culture conditions that allow substantial amplification of transient engrafting murine stem cells and which, simultaneously, augment the ability to sustain serial bone marrow transplantation (BMT). Short-term incubation of unfractionated BM cells in liquid culture with stem cell factor (SCF) and interleukin-11 (IL-11) produced a 50-fold amplification of clonogenic multipotential progenitors (CFU-A). Following such ex vivo expansion, substantially fewer cells were required to rescue lethally irradiated mice. When transplanted in cell doses above threshold for engraftment, BM cells expanded ex vivo resulted in significantly more rapid hematopoietic recovery. In a serial transplantation model, unmanipulated BM was only able to consistently sustain secondary BMT recipients, but BM expanded ex vivo has sustained quaternary BMT recipients that remain alive and well more than 140 days after 4th degree BMT. These results show augmentation of both short-term recovery posttransplant and the ability to serially transplant marrow by preincubation in culture with SCF and IL-11.     

浅谈EPC总承包工程项目质量管理

结合EPC工程项目管理特点，将质量管理的基本要素充分体现在每一个工作环节，确保工程质量符合规程规范和业主质量目标要求，为机组今后正常稳定运行奠定坚实的基础。     

直流非转移弧等离子喷枪中电弧的工作特性研究

利用计算机数值模拟技术，采用有限容积方法，针对自制的低能耗、高效率内送粉等离子喷涂设备，对喷枪内部电弧长度及其对流体特性的影响进行了分析研究。结果表明，通过增加进气流量和减少电流的大小，均使得等离子电弧长度增加，阳极弧根位置逐渐向下游移动，但是产生这两种现象的机理以及对气流温度和速度的影响是不同的。     

主动式红外光电靶的研究

提出了采用红外光源的主动式光电靶的设计构想，用以消除传统天幕靶在对低速弹测量时，声音对测量的干扰，并设计了整个实验方案，在对静态实验结果进行分析的基础上，证明了该种方法的可行性。     

黑龙江省"农业需水"初探

黑龙江省复种指致为1，推算农业需水为400～500mm，而多年平均年降水量多数大于400mm，有发展雨养农业可能；三江平原多年平均年降水量550mm，为此三江平原井灌区现有耕地具有全部发展种植水稻的可能。     

学习者焦虑感之成因及其消除策略

多数学习者的成绩与其性格有关。那些性格内向、缺乏自信心者常常产生焦虑感，羞于开口，担心犯错误，进而影响学习成绩。文章就学习者焦虑感的产生与课堂教学活动之间的相互作用进行了探讨，并在此基础上提出了如何减少或消除焦虑感的策略。