Genomics and Epigenomics of Gestational Diabetes Mellitus: Understanding the Molecular Pathways of the Disease Pathogenesis

One of the most common complications during pregnancy is gestational diabetes mellitus (GDM), hyperglycemia that occurs for the first time during pregnancy. The condition is multifactorial, caused by an interaction between genetic, epigenetic, and environmental factors. However, the underlying mechanisms responsible for its pathogenesis remain elusive. Moreover, in contrast to several common metabolic disorders, molecular research in GDM is lagging. It is important to recognize that GDM is still commonly diagnosed during the second trimester of pregnancy using the oral glucose tolerance test (OGGT), at a time when both a fetal and maternal pathophysiology is already present, demonstrating the increased blood glucose levels associated with exacerbated insulin resistance. Therefore, early detection of metabolic changes and associated epigenetic and genetic factors that can lead to an improved prediction of adverse pregnancy outcomes and future cardio-metabolic pathologies in GDM women and their children is imperative. Several genomic and epigenetic approaches have been used to identify the genes, genetic variants, metabolic pathways, and epigenetic modifications involved in GDM to determine its etiology. In this article, we explore these factors as well as how their functional effects may contribute to immediate and future pathologies in women with GDM and their offspring from birth to adulthood. We also discuss how these approaches contribute to the changes in different molecular pathways that contribute to the GDM pathogenesis, with a special focus on the development of insulin resistance.     

The Plasminogen–Activator Plasmin System in Physiological and Pathophysiological Angiogenesis

Angiogenesis is a process associated with the migration and proliferation of endothelial cells (EC) to form new blood vessels. It is involved in various physiological and pathophysiological conditions and is controlled by a wide range of proangiogenic and antiangiogenic molecules. The plasminogen activator–plasmin system plays a major role in the extracellular matrix remodeling process necessary for angiogenesis. Urokinase/tissue-type plasminogen activators (uPA/tPA) convert plasminogen into the active enzyme plasmin, which in turn activates matrix metalloproteinases and degrades the extracellular matrix releasing growth factors and proangiogenic molecules such as the vascular endothelial growth factor (VEGF-A). The plasminogen activator inhibitor-1 (PAI-1) is the main inhibitor of uPA and tPA, thereby an inhibitor of pericellular proteolysis and intravascular fibrinolysis, respectively. Paradoxically, PAI-1, which is expressed by EC during angiogenesis, is elevated in several cancers and is found to promote angiogenesis by regulating plasmin-mediated proteolysis and by promoting cellular migration through vitronectin. The urokinase-type plasminogen activator receptor (uPAR) also induces EC cellular migration during angiogenesis via interacting with signaling partners. Understanding the molecular functions of the plasminogen activator plasmin system and targeting angiogenesis via blocking serine proteases or their interactions with other molecules is one of the major therapeutic strategies scientists have been attracted to in controlling tumor growth and other pathological conditions characterized by neovascularization.     

Mathematical Model for Isothermal Wire-Coating From a Bath of Giesekus Viscoelastic Fluid

A mathematical model of wire-coating based on Giesekus constitutive equation is analyzed under isothermal conditions. It is desired to see the functional dependence of Giesekus model parameters on the important operating variables in the wire-coating process, which include volumetric flow rate (later referred to as flow rate), shear stress at the wire surface (later referred to as shear stress), force required for pulling the wire (later referred to as force), and radius of the coated wire (later referred to as coated wire thickness). To this end, the equation governing the laminar, incompressible, and rectilinear flow is first derived and then solved analytically for the case of vanishing axial pressure gradient. A numerical procedure is described to obtain the solution for the case of nonvanishing pressure gradient. Our results indicate that the magnitude of shear stress and force follow a decreasing trend with increasing Giesekus model parameters in both cases. The flow rate and coated wire thickness decrease on increasing the Giesekus model parameters when there is no imposed pressure gradient. However, in the presence of pressure gradient these variables first decrease with increasing Giesekus model parameters and then follow an increasing trend.     

Innovative nano silicone wax coatings for the conservation of bronze artifacts from corrosion

Journal of Applied Electrochemistry - Herein we studied the efficiency of a protective transparent and reversible nano silicone wax coatings (NSW) to protect bronze artifacts extracted from...     

Effect of multipath fading and multiple access interference on broadband code division multiple access systems

In this paper, the performance of uncoded uplink transmissions in a broadband code division multiple access (CDMA) system using tapped delay line (TDL) antenna array under multipath conditions is investigated. An expression for broadband CDMA system is derived to calculate the optimal weights of TDL antenna array. Using the derived broadband expression, the performance of TDL antenna array is investigated and compared with that of the already existing narrowband systems. Simulation results show that TDL antenna array can reduce multipath fading and multiple access interference (MAI). It is shown that if the signal‐to‐noise ratio (SNR) level is maintained at 20 dB and two‐ray channel model is used, two antennas with four taps each can support 30 users with a BER of 10^?3. If three‐ray channel model is used, the same antenna arrangement is not enough to support 30 users. To support these 30 users with BER of 10^?3, the number of taps needs to be increased to eight. Finally, the number of taps needs to be further increased to 16 if four‐ray channel model is used. Not only that, the SNR should also be increased to 25 dB to achieve the same BER. Copyright © 2011 John Wiley & Sons, Ltd.     

Implications of the UK field trial of building mounted horizontal axis micro-wind turbines

Building mounted micro-wind turbines and photovoltaics have the potential to provide widely applicable carbon free electricity generation at the building level. Photovoltaic systems are well understood and it is easy to predict performance using software tools or widely accepted yield estimates. Micro-wind, however, is far more complex and in comparison poorly understood. This paper presents the key findings of the building mounted (<2 kWp) turbine component of the UK micro-wind trial undertaken by the Energy Saving Trust in 2008/09. The monitored performance of 39 horizontal axis turbines in urban, suburban and rural locations is discussed alongside the accuracy of predictive wind speed tools for the sites. The performance of urban and suburban micro-wind sites in the trial was poor with annual generation of less than 75 kWh/m² swept area, the majority of which were less than 25 kWh/m². Good rural sites had an annual generation of between 100 and 280 kWh/m², far less than the nominal 360 kWh/m² (10% load factor for a typical turbine) that is often assumed. In the light of these findings, the potential impact of the UK’s latest policy instrument, the 2010 micro-generation tariffs, is considered for both micro-wind and photovoltaics.     

The radial spreading as a free surface layer of a vertical buoyant jet

Summary When the rising plume in a stagnant ambient fluid impinges the free surface it spreads radially as a surface layer flowing over a pool of heavier liquid. Initially the surface layer in the entrainment zone entrains the underlying fluid, but the entrainment decreases with the increasing distance from the boil. The radius of the entrainment zone depends on the initial buoyancy flux from the nozzle and also on submergence height. It was assumed that the flow in this zone has a self-preserving form. The theoretical results based upon this assumption showed that the maximum velocity and density difference of the layer decreases as powers of radius distance measured from centre of the boil and the depth varies linearly with the radius, so that the sectional Richardon's number based on maximum physical quantities of the section remain unchanged. It was further shown that the coefficient of the entrainment remains constant and is a function of the Richardson's number and the slope of the interface. The theoretical results were compared with those obtained experimentally and fair agreement was obtained.At the end of the entrainment zone the surface layer enters the zone of homogeneous flow in which the layer travels over the underlying fluid and behaves much like a free homogeneous flow over a rigid boundary: the friction at the interface replacing the friction of the boundary. In this zone the mean velocity of the layer in the radial direction was measured and found that it decreases inversely with the increase of radius. The thickness of the layer in the zone of homogeneous flow remains constant and depends, as far as experiments show, greatly on the submergence height.     

Exploring the conformational space of cyclic peptides by a stochastic search method

A stochastic search algorithm is applied in order to probe the conformations of cyclic peptides. The search is conducted in two stages. In the first stage, random conformations are generated and evaluated by a penalty function for ring closure ability, following a stepwise construction of each amino acid into the peptide by a random choice of one of its allowed conformations. The allowed conformational ranges of backbone dihedral angles for each amino acid have been extracted from a Data Bank of diverse proteins. Values of dihedral angles that do not contribute favorably to the scoring of ring closure are retained or discarded by a statistical test. Values are discarded up to a point from which all remaining combinations of angles are constructed, scored, sorted, and clustered. In the second stage, side chains have been added and fast optimization was applied to the set of diverse conformations in a "united atoms" approach, with the "Kollman forcefield" of Sybyl 6.8. This iterative stochastic elimination algorithm finds the global minimum and most of the best results, when compared to a full exhaustive search in appropriately sized problems. In larger problems, we compare the results to experimental structures. The root mean square deviation (RMSD) of our best results compared to crystal structures of cyclic peptides with sizes from 4 to 15 amino acids are mostly below 1.0 A up to 8 mers and under 2.0 A for larger cyclic peptides.     

An Antilock-Braking Algorithm for an Eddy-Current-Based Brake-By-Wire System

This paper presents a nonlinear sliding-mode-type controller for slip regulation in a braking event for an electromagnetic brake-by-wire (BBW)-system-equipped automobile. The electromagnetic BBW system under consideration consists of a set of eddy current brakes (ECBs) attached to the automobile wheels. The antilock-braking controller modifies the brake torque command generated by a supervisory controller which, in turn, is based on the driver's command sensed via brake pedal sensors. The modified brake torque command is then generated by a closed-loop actuator control algorithm to control the ECB system. It is shown in this paper that the proposed antilock-braking control system is stable in the sense of Lyapunov. Experimental results are presented for a test vehicle equipped with an eddy-current-based BBW system. Experimental results show that the proposed antilock brake control algorithm provides very good slip regulation in a braking event on low friction-coefficient surfaces (wet jennite) when compared with that of a braking event without the proposed antilock-braking control. Experimental results also indicate that the proposed antilock-braking control system provides a smooth stop for the vehicle     

An Empirical Study of Factors Affecting e-Commerce Adoption among Small- and Medium-Sized Enterprises in a Developing Country: Evidence from Malaysia

The purpose of this paper is to empirically examine determinants of e-commerce adoption among Malaysian small- and medium-sized enterprises (SMEs). This study tested eight hypotheses on determinants affecting e-commerce adoption with empirical data from several survey instruments: an online questionnaire-based survey, mailed survey and questionnaire collected in-person from a sample of 307 SMEs in Malaysia. The findings show that e-commerce adoption within Malaysian SMEs is affected by perceived relative advantage, perceived compatibility, managers/owner's knowledge and expertise, management characteristics and external change agents. This study contributes to enhancing the understanding of the determinants of adopting e-commerce in SMEs and provides some interesting perspective from Malaysia. Those parties interested in promoting their business online may find these results helpful in guiding their efforts.