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AP van Nieuwstadt JJ Meulenberg A van Essen-Zanbergen A Petersen-den Besten RJ Bende RJ Moormann G Wensvoort 《Canadian Metallurgical Quarterly》1996,70(7):4767-4772
Four structural proteins of Lelystad virus (Arteriviridae) were recognized by monoclonal antibodies in a Western immunoblotting experiment with purified virus. In addition to the 18-kDa integral membrane protein M and the 15-kDa nucleocapsid protein N, two new structural proteins with molecular masses of 45 to 50 kDa and 31 to 35 kDa, respectively, were detected. Monoclonal antibodies that recognized proteins of 45 to 50 kDa and 31 to 35 kDa immunoprecipitated similar proteins expressed from open reading frames (ORFs) 3 and 4 in baculovirus recombinants, respectively. Therefore, the 45- to 50-kDa protein is encoded by ORF3 and the 31- to 35-kDa protein is encoded by ORF4. Peptide-N-glycosidase F digestion of purified virus reduced the 45- to 50-kDa and 31- to 35-kDa proteins to core proteins of 29 and 16 kDa, respectively, which indicates N glycosylation of these proteins in the virion. Monoclonal antibodies specific for the 31- to 35-kDa protein neutralized Lelystad virus, which indicates that at least part of this protein is exposed at the virion surface. We propose that the 45- to 50-kDa and 31- to 35-kDa structural proteins of Lelystad virus be named GP3 and GP4, to reflect their glycosylation and the ORFs from which they are expressed. Antibodies specific for GP3 and GP4 were detected by a Western immunoblotting assay in swine serum after an infection with Lelystad virus. 相似文献
74.
柏杖子金矿100-9#矿体为残矿,内部存在多个空区,矿岩稳定性差,岩移危害大,遗留有大量矿石散体,致使矿体回收困难。为此,提出多分段与底部双堑沟协同拉底的诱导冒落法回采方案,在分析矿岩可冒性、临界冒落跨度和冒落高度随采深的关系的基础上,结合矿体赋存特点及矿石流动特性,给出了诱导工程参数及爆破参数;采用矿体中部做切割向两端退采的方式,在满足上分段回采工作面超前下分段6~8个步距前提下,各分段拉底巷道同时施工,逐次诱导上部待冒矿体,由双堑沟整体回收,同时选用喷锚网联合支护方式,冒落垫层取3 m,可安全、经济、有效地回收残矿。 相似文献
75.
H. Bende 《European Journal of Lipid Science and Technology》1968,70(12):937-940
Separation of Mixtures of Lipophilic Substances by Gel-Filtration The opportunities for the separation of lipophilic substances by gel-filtration, a form of liquid chromatography based on the principle of molecular sieving, was very little utilized till now, because no effective molecular sieves were available. The newly developed lipophilic-hydrophilic molecular sieve Sephadex LH-20 enables separations of fractions upto a molecular weight of 5000 under usual conditions. With complicated mixtures the efficiency of separation can be increased by coupling the gelfiltration with partition chromatography and by employing the gradient elution technique. 相似文献
76.
为深入研究新的水库防洪调度设计理论方法,提高防洪安全度和洪水资源利用率,本文在作者以往防洪分类调度方式研究的基础上,系统的论述了分类调度方式设计的必要性和可行性,基本理念、假定,实施防洪分类调度方式设计的条件与步骤。并以碧流河水库为例,论述其分类调度规则的制定过程,并检验其防洪分类调度方式设计的合理性与安全性。 相似文献
77.
保护性古旧建筑的加固改造 总被引:5,自引:0,他引:5
李本德 《哈尔滨建筑大学学报》1995,28(4):128-132
通过哈尔滨和平屯宾馆古保建筑的加固改造,提出对保护性古旧建筑的鉴定,检查及加固改造的技术措施和方法,可供同类工程参考。 相似文献
78.
By having professional wine tasters and controls perform olfactory tasks of absolute detection (1-butanol), discrimination (lemon and cloves), and identification (common household odors), the present two experiments studied (a) if perceptual odor learning takes place from odor experience acquired under nonlaboratory conditions, (b) if this learning generalizes to odors for which experience is limited, and (c) if generalized learning can be referred to increased general interest for odors that increases attention to odorous features. The results showed that whereas wine tasters were not better than controls on detection, they were superior to controls on discrimination and identification, the latter due to only a few odors. Ratings of experience with certain odors during professional evaluation suggest that generalized perceptual learning may take place in discrimination but not in identification. Wine tasters did not show more general interest for odorous features than did controls. The nonsuperiority in detection may be explained by the fact wine tasters have no professional experience of a detection task per se, implying that perceptual odor learning does not generalize from the olfactory tasks of discrimination and identification to detection. 相似文献
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本文针对某款小型纯电动汽车,对其动力系统的重要参数进行了设计和匹配。定义了传动比设计常数,给出了传动比设计的方法和步骤。在AVL CRUISE和MATLAB中建立了模型并分别从动力性和经济性方面进行了联合仿真。结果表明动力性和经济性完全满足设计要求,本文对如何设计动力系统参数具有重要意义。 相似文献