Removal efficiency of subsurface vertical flow constructed wetlands for different organic loads.

Using subsurface vertical flow constructed wetlands (SSVFCWs) with intermittent loading it is possible to fulfil the stringent Austrian effluent standards regarding nitrification. For small plants (less than 500 persons) standards for ammonia nitrogen concentration have to be met at water temperatures higher than 12 degrees C, effluent concentrations and treatment efficiencies for organic matter have to be met the whole year around. According to the Austrian design standards the required surface area for SSVFCWs treating wastewater was 5 m2 per person. Within the first part of an Austrian research project the goal was to optimise, i.e. minimise the surface area requirement of vertical flow beds. Therefore, three SSVFCWs with a surface area of 20 m2 each have been operated in parallel. The organic loads applied were 20, 27 and 40 g COD/m2/d, which corresponds to a specific surface area requirement of 4, 3 and 2 m2 per PE, respectively. The paper compares the effluent concentrations and elimination efficiencies of the three parallel operated beds. It could be shown that a specific area demand of 4 m2 per person is suitable to be included in the revision of the Austrian design standard. Additionally it could be shown that during the warmer seasons (May-October) when the temperature of the effluent is higher than 12 degrees C the specific surface area might be further reduced; even 2 m2 per person has been proven to be adequate.     

The beneficial effects of storage on the quality of wastewater for irrigation: a case study in Sicily.

Although the main function of reservoirs in agriculture is to allow the storage and temporal shifting of large volumes of waters, further benefits emerge from their application in wastewater reuse systems. In fact, as observed in several other studies carried out on such complex natural systems, the storage period, under proper conditions, can lead to a significant improvement of the water quality for irrigation thanks to concurrent physical, chemical and biological processes. In order to investigate the reservoir dynamics, an experimental research was carried out in a farm reservoir (volume of 11,000 m3, maximum depth of 6.3 m and mean depth of about 5 m), receiving treated wastewater and subject to scheduled highly variable input to volume ratios, in order to observe the broadest range of behaviour responses. Specifically, over a monitoring period lasted 4 months, the reservoir was operated as a standard batch reactor in the first and third phases and as a continuous flow reactor in the second one. Based on correlation analysis between the chemical, physical and biological water parameters and operational ones, a new operative index is proposed which integrates the morphological characteristics of the tank and the management ones, and shows a significant correlation with the qualitative characteristics of the stored water.     

Hindered and compression settling: parameter measurement and modelling.

The Vesilind settling velocity function forms the basis of flux theory used both in state point analysis (for design and capacity rating) and one-dimensional dynamic models (for dynamic process modelling). This paper proposes new methods to address known shortcomings of these methods, based on an extensive set of batch settling tests conducted at different scales. The experimental method to determine the Vesilind parameters from a series of bench scale settling tests is reviewed. It is confirmed that settling cylinders must be slowly stirred in order to represent settling performance of full scale plants for the whole range of solids concentrations. Two new methods to extract the Vesilind parameters from settling test series are proposed and tested against the traditional manual method. Finally, the same data set is used to propose an extension to one-dimensional (1-D) dynamic settler models to account for compression settling. Using the modified empirical function, the model is able to describe the batch settling interface independently of the number of layers.     

The herbicide propachlor--14 C synthesis, toxicologic problems and methodology studies on the determination of residues in corn, potatoes, water and soil

Using the synthesized agent propachlor (N-isopropyl-2-chloroacetanilid) labelled with 14C, methodical investigations revealed an extremely high steam volatility not being assumed before because of the low vapour pressure. The method of steam distillation basing on this fact is of universal application to the processing of maize, potatoes, water, and soil, in which propachlor could be quantitatively determined by gas chromatography with a detection sensitivity of 0.02 ppm. Furthermore, this methods allows a simple purification of the raw product of synthesis containing impurities up to 20%. Propachlor codistilled with steam had a purity of more than 99%. Possibly, the high steam volatility of propachlor could have economic and toxicological consequences as to its application (volatilization in the open air with a high atmospheric humidity along with high temperatures). Therefore, an investigation of the steam volatility in the general screening programme for new agents is to be recommended in addition to the usual investigations of steam pressure.     

Stoichiometric dye-binding procedure for the determination of the reactive lysine content of soya bean protein

A new analytical method has been developed for the determination of the reactive lysine content of soya bean protein. The method is based on the reaction of the free basic groups of the protein with 1-phenylazo-2 naphthol-6,8 disulphonic acid. With regard to the stoichiometry of the procedure, it has been proved, contrary to earlier reports, that the basic amino acids, histidine, arginine and lysine, each combine with one mole of the dye. After acylation with propionic anhydride lysine alone loses its dye reactivity. The usefulness of the proposed method has been demonstrated by the determination of the reactive lysine content of several untreated, heat-treated and acid-treated soya bean samples. The results show that heat damage of about 5% in reactive lysine content can be measured in 1·5 h with good reproducibility.     

Effect of Methionine Supplementation on Nitrogen Balance in Infants Fed a Low-Cost Soy-Oats Formula

A nitrogen balance study, comparing a low-cost soy-oats infant formula with and without supplemental methionine, was carried out with infants of ages 3 - 26 months at a single equivalent level of nitrogen intake (approximately 850 mg/kg/day). Nitrogen retention was found to be unaffected by methionine supplementation. Com- parison of data obtained in this study with previous research re- vealed that a critical value for intake of total L-sulfur amino acids apparently exists in infants 3 - 26 months of age, below which methionine supplementation will increase nitrogen retention, but above which it will not. This value appears to coincide with that of 49 mg/kg/day recommended for infants by the FNB.