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201.
Multiple episodes of blood-brain barrier disruption were induced by sequential intraspinal injections of ethidium bromide. In addition to the barrier disruption, there was toxic demyelination and exposure of myelin components to the immune system. Twenty-seven 3-month-old Wistar rats received 2, 3 or 4 injections of 1 microliter of either 0.1% ethidium bromide in normal saline (19 rats) or 0.9% saline (8 rats) at different levels of the spinal cord. The time intervals between the injections ranged from 28 to 42 days. Ten days after the last injection, all rats were perfused with 2.5% glutaraldehyde. The spinal sections were evaluated macroscopically and by light and transmission electron microscopy. All the lesions demonstrated a mononuclear phagocytic infiltrate apparently removing myelin. Lymphocytes were not conspicuous and were found in only 34% of the lesions. No perivascular cuffings were detected. In older lesions (38 days and older) they were found only within Virchow-Robin spaces. This result suggests that multiple blood-brain barrier disruptions with demyelination and exposure of myelin components to the immune system were not sufficient to induce an immune-mediated reaction in the central nervous system. 相似文献
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Studies under controlled conditions of lighting and temperature revealed clear evidence of circadian periodicity with respect to the number of PFC present in the spleens of BALB/c mice 3 or 4 days after immunization with SRBC. Striking differences in proliferative responses of spleen lymphocytes to PHA or Con A were also observed at two different circadian times. Large proliferative responses occurred at the time when injection of antigen and/or sampling for PFC yielded a low PFC formation (early in the daily dark span) and small proliferative responses occurred at the time when antigen injection and sampling yielded high formation of PFC (early in the daily light span). These findings indicate that care should be taken to control the circadian timing of stimulation and sampling in studies of immune responses, and that rhythmically varying aspects constitute a new dimension of immunologic processes awaiting further analysis in both the circadian and weekly spectral regions. 相似文献
207.
Lake IR Foxall CD Lovett AA Fernandes A Dowding A White S Rose M 《Environmental science & technology》2005,39(23):9033-9038
This paper presents the results of a study examining whether the flooding of pasture by rivers gives rise to higher PCDD/F and PCB concentrations in cows' milk. Over 180 milk, soil, and grass samples, taken from 38 farms across 3 different river systems (River Dee, Trent, and Doe Lea/Rother/Don) in the United Kingdom, were analyzed for PCDD/Fs and PCBs. The concentrations were compared between flood-prone farms, where the animals had access to pasture that is often flooded, and control farms where the land does not flood. The results indicated that concentrations of PCDD/Fs and PCBs in cows' milk were higher in samples taken from farms prone to flooding, but only from the river systems flowing through industrial and urban areas. Raised levels of PCDD/F and PCBs were also found in soil and grass from farms prone to flooding providing strong corroborative evidence that the higher concentrations in cows' milk from such areas is likely to be due to the ingestion of contaminated grass and soil. Overall, the results provide strong evidence that flooding of pastureland can indeed result in elevated concentrations of PCDD/Fs and PCBs in milk from the farms so affected. 相似文献
208.
JC Fernandes D Jovanovic F Dehnade J Martel-Pelletier JP Pelletier 《Canadian Metallurgical Quarterly》1998,52(8):768-775
INTRODUCTION AND GOAL: The dog model of osteoarthritis is the one most used for the study of the pathophysiology of this disease, given its resemblance to human osteoarthritis. Although the percutaneous section of the ligament is the most known technique, variability of and iatrogenic lesions might occur. The goal of this study was to validate the arthroscopic technique regarding the osteaorthritic lesions found in the percutaneous Pond-Nuki dog model. METHODS: The arthroscopic section of the canine anterior cruciate ligament (ACL) was compared to the percutaneous technique. Six dogs were operated percutaneously (Group I) and another six by arthroscopy (Group II). At eight weeks, animals were sacrificed and analysis were done on the osteophytes (mm) and cartilage lesions [macroscopy (Pelletier scale: grade 0-4, and size, mm2)] and [microscopy (Mankin scale: 0-14)] on femoral condyles and tibial plateaus. RESULTS: Osteophytes (Group I: 3.4 mm, Group II: 4.4 mm) and cartilage lesions (grade: 2.07 and 1.67; size: 27.9 and 33.9 m, respectively) on both femoral condyles and tibial plateaus are similar and not statistically different. Total microscopic scoring for both groups (6.2 and 6.5, respectively) are also similar and not statistically significant. DISCUSSION: Experimental osteoarthritis induced by arthroscopic transection of the ACL in dogs is characterised by macroscopic and microscopic lesions that resemble those found after percutaneous stab transection. Arthroscopic technique is more reliable and the present results allow its validation regarding the classical percutaneous technique. 相似文献
209.
Ana B. Dias Natlia T. Correia Joaquim J. Moura-Ramos Anabela C. Fernandes 《Polymer International》1994,33(3):293-302
The dipolar relaxation mechanisms in poly(vinyl acetate) have been studied in detail using the technique of thermally stimulated currents. The papers published in the literature about this subject are very contradictory, particularly with respect to the assignment of the observed discharges to the corresponding motions at the molecular level. This work aims at clarifying these problems. We detected and characterised three different relaxation mechanisms: (1) a low temperature one (around ?140°C) which was attributed to local internal rotations in the acetate side-groups; (2) a relaxation whose maximum occurs at 42°C, which corresponds to the glass transition relaxation, and shows a compensation behaviour; (3) an upper glass transition relaxation whose maximum appears at 87°C and was attributed to a liquid-liquid transition. These assignments have been made on the basis of the analysis of the behaviour of the samples when submitted to different thermal and electrical treatments. 相似文献
210.
Conjugated linoleic acid and chromium lower body weight and visceral fat mass in high-fat-diet-fed mice 总被引:1,自引:1,他引:0
More than half of the U.S. population has a body mass index of 25 kg/m2 or more, which classifies them as overweight or obese. Obesity is often associated with comorbidities such as diabetes, cardiovascular
diseases, and cancer. CLA and chromium have emerged as major dietary supplements that reduce body weight and fat mass, and
increase basal metabolic rate in animal models. However, studies show that CLA induces insulin resistance in mice and in humans,
whereas Cr improves insulin sensitivity. Hence, we designed the present study to examine the combined effect of CLA and Cr
on body composition and insulin sensitivity in a Balb/c mice (n=10/group) model of high-fat-diet-induced obesity. CLA alone lowered body weight, total body fat mass, and visceral fat mass,
the last of which decreased further with the combination of CLA and Cr. This effect was accompanied by decreased serum leptin
levels in CLA-fed and CLA+Cr-fed mice, and by higher energy expenditure (EE) and oxygen consumption (OC) in CLA+Cr-fed mice.
Serum levels of glucose, insulin, the pro-inflammatory cytokines, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6),
as well as insulin resistance index (IRI), decreased with CLA, whereas CLA and Cr in combination had significant effects on
insulin and IL-6 concentrations and IRI. In summary, CLA+Cr decreased body weight and fat mass in high-fat-diet-fed mice,
which may be associated with decreased leptin levels and higher EE and OC. 相似文献