The use of a dome osteotomy in the treatment of infantile tibia vara is reportedly associated with insufficient intraoperative assessment of the correction achieved. This article discusses a method to overcome this problem and the long-term advantages of using a dome osteotomy in the treatment of this disease. 相似文献
Temperature-regulated expression of recombinant proteins in the tac promoter (Ptac) system was investigated. Expression levels of fungal xylanase and cellulase from N. patriciarum in E. coli strains containing the natural lacI gene under the control of the Ptac markedly increased with increasing cultivation temperature in the absence of a chemical inducer. The specific activities (units per milligram protein of crude enzyme) of the fungal xylanase and cellulase produced from recombinant E. coli strain pop2136 grown at 42 degrees C were about 4.5 times higher than those of the cells grown at 23 degrees C and were even slightly higher when compared with cells grown in the presence of the inducer isopropyl beta-D-thiogalactopyranoside. The xylanase expression level in the temperature-regulated Ptac system was about 35% of total cellular protein. However, this system can not be applied to E. coli strains containing lacIq, which confers over production of the lac repressor, for high-level expression of recombinant proteins. In comparison with the lambda PL system, the Ptac-based xylanase plasmid in E. coli pop2136 gave a considerably higher specific activity of the xylanase than did the best lambda PL-based construct using the same thermal induction procedure. The high-level expression of the xylanase using the temperature-regulated Ptac system was also obtained in 10-litre fermentation studies using a fed-batch process. These results unambiguously demonstrated that the temperature-modulated Ptac system can be used for overproduction of some non-toxic recombinant proteins. 相似文献
The in situ forces and their distribution within the human anterior cruciate ligament (ACL) can clarify this ligament's role in the knee and help to resolve controversies regarding surgical treatment of ACL deficiency. We used a universal force-moment sensor (UFS) to determine the magnitude, direction, and point of application of the in situ forces in the ACL in intact human cadaveric knees. Unlike previous studies, this approach does not require surgical intervention, the attachment of mechanical devices to or near the ACL, or a priori assumptions about the direction of in situ force. Anterior tibial loads were applied to intact knees, which were limited to 1 degree of freedom at 30 degrees flexion. The in situ forces developed in the ACL were lower than the applied force for loads under 80 N, but larger for applied loads of more than 80 N. The direction of the force vector corresponded to that of the anteromedial (AM) portion of the ACL insertion on the tibial plateau. The point of force application was located in the posterior section of the anteromedial portion of the tibial insertion site. The anterior and posterior aspects of the anteromedial portion of the ACL supported 25% and 70% of the in situ force, respectively, with the remainder carried by the posterolateral portion. We believe that the data obtained with this new UFS methodology improves our understanding of the role of the ACL in knee function, and that this methodology can be easily extended to study the function of other ligaments. 相似文献
根据信道冲激响应的稀疏特性,提出了一种频域的时延估计压缩感知模型,将时延估计问题转化为基于欠采样数据的稀疏向量估计问题.利用离散傅里叶变换(Discrete Fourier transform,DFT)矩阵的子矩阵所满足的受限等距性(Restricted isometry property,RIP)以及信道冲激响应的稀疏特性充分降低了时延估计所需数据量的要求.分析了本文模型具有码片内多径分辨能力以及良好抗噪性能的原因,并与多信号分类(Multiple signal classification,MUSIC)和旋转不变技术的信号参数估计(Estimation of signal parameters via rotational invariance technique,ESPRIT)算法的时延估计性能进行仿真比较.仿真结果表明,本文提出的方法不需要预知多径的条数,对码片内多径时延具有较高的估计精度,其时延估计性能在特定条件下优于MUSIC和ESPRIT算法. 相似文献
Surrogate model-assisted multi-objective genetic algorithms (MOGA) show great potential in solving engineering design problems since they can save computational cost by reducing the calls of expensive simulations. In this paper, a two-stage adaptive multi-fidelity surrogate (MFS) model-assisted MOGA (AMFS-MOGA) is developed to further relieve their computational burden. In the warm-up stage, a preliminary Pareto frontier is obtained relying only on the data from the low-fidelity (LF) model. In the second stage, an initial MFS model is constructed based on the data from both LF and high-fidelity (HF) models at the samples, which are selected from the preliminary Pareto set according to the crowding distance in the objective space. Then the fitness values of individuals are evaluated using the MFS model, which is adaptively updated according to two developed strategies, an individual-based updating strategy and a generation-based updating strategy. The former considers the prediction uncertainty from the MFS model, while the latter takes the discrete degree of the population into consideration. The effectiveness and merits of the proposed AMFS-MOGA approach are illustrated using three benchmark tests and the design optimization of a stiffened cylindrical shell. The comparisons between the proposed AMFS-MOGA approach and some existing approaches considering the quality of the obtained Pareto frontiers and computational efficiency are made. The results show that the proposed AMFS-MOGA method can obtain Pareto frontiers comparable to that obtained by the MOGA with HF model, while significantly reducing the number of evaluations of the expensive HF model.
Applied Intelligence - Personnel performance is a key factor to maintain core competitive advantages. Thus, predicting personnel future performance is a significant research domain in human... 相似文献