腰果酚聚氧乙烯醚对甲磺酸体系电镀锡的影响

通过线性扫描伏安法、循环伏安法和计时电流法研究了腰果酚聚氧乙烯醚(BGF)对甲磺酸体系中锡电沉积的影响,并分析了BGF对电镀锡层表面形貌和相结构的影响.结果表明,BGF能够抑制Sn2+的还原反应和传质扩散,改变锡晶粒成核过程,起到细化晶粒的作用,最终得到细致、平整的锡镀层.     

考虑寄生参数的隔离型双有源桥DC-DC变换器的开关开路故障分析与容错控制策略

双有源桥(DAB)DC-DC变换器开关管的驱动电路故障导致开关开路故障(OCSF),这使得DAB系统拓扑对称性缺失、高频电压和电流发生畸变和直流偏置,进而对系统的可靠性和长期运行稳定性造成不良影响.对此,研究DAB开关管和高频变压器寄生参数对OCSF后系统运行状态的影响,以完善DAB故障分析体系.针对OCSF问题,提出一种基于对称拓扑和对偶原理的故障容错策略,通过闭锁开关使系统运行拓扑恢复对称,并在此基础上提出一种不改变控制器参数、只需改变控制器输出饱和参数的实用控制方法,并在理论上讨论了该方法的可行性.最后,通过实验验证了OCSF模态分析及所提容错控制策略的正确性.     

Isolation and Functional Characterization of a Salt-Responsive Calmodulin-Like Gene MpCML40 from Semi-Mangrove Millettia pinnata

Salt stress is a major increasing threat to global agriculture. Pongamia (Millettia pinnata), a semi-mangrove, is a good model to study the molecular mechanism of plant adaptation to the saline environment. Calcium signaling pathways play critical roles in the model plants such as Arabidopsis in responding to salt stress, but little is known about their function in Pongamia. Here, we have isolated and characterized a salt-responsive MpCML40, a calmodulin-like (CML) gene from Pongamia. MpCML40 protein has 140 amino acids and is homologous with Arabidopsis AtCML40. MpCML40 contains four EF-hand motifs and a bipartite NLS (Nuclear Localization Signal) and localizes both at the plasma membrane and in the nucleus. MpCML40 was highly induced after salt treatment, especially in Pongamia roots. Heterologous expression of MpCML40 in yeast cells improved their salt tolerance. The 35S::MpCML40 transgenic Arabidopsis highly enhanced seed germination rate and root length under salt and osmotic stresses. The transgenic plants had a higher level of proline and a lower level of MDA (malondialdehyde) under normal and stress conditions, which suggested that heterologous expression of MpCML40 contributed to proline accumulation to improve salt tolerance and protect plants from the ROS (reactive oxygen species) destructive effects. Furthermore, we did not observe any measurable discrepancies in the development and growth between the transgenic plants and wild-type plants under normal growth conditions. Our results suggest that MpCML40 is an important positive regulator in response to salt stress and of potential application in producing salt-tolerant crops.     

Design and Synthesis of a Novel PLK1 Inhibitor Scaffold Using a Hybridized 3D-QSAR Model

Polo-like kinase 1 (PLK1) plays an important role in cell cycle progression and proliferation in cancer cells. PLK1 also contributes to anticancer drug resistance and is a valuable target in anticancer therapeutics. To identify additional effective PLK1 inhibitors, we performed QSAR studies of two series of known PLK1 inhibitors and proposed a new structure based on a hybridized 3D-QSAR model. Given the hybridized 3D-QSAR models, we designed and synthesized 4-benzyloxy-1-(2-arylaminopyridin-4-yl)-1H-pyrazole-3-carboxamides, and we inspected its inhibitory activities to identify novel PLK1 inhibitors with decent potency and selectivity.     

Usher Syndrome in the Inner Ear: Etiologies and Advances in Gene Therapy

Hearing loss is the most common sensory disorder with ~466 million people worldwide affected, representing about 5% of the population. A substantial portion of hearing loss is genetic. Hearing loss can either be non-syndromic, if hearing loss is the only clinical manifestation, or syndromic, if the hearing loss is accompanied by a collage of other clinical manifestations. Usher syndrome is a syndromic form of genetic hearing loss that is accompanied by impaired vision associated with retinitis pigmentosa and, in many cases, vestibular dysfunction. It is the most common cause of deaf-blindness. Currently cochlear implantation or hearing aids are the only treatments for Usher-related hearing loss. However, gene therapy has shown promise in treating Usher-related retinitis pigmentosa. Here we review how the etiologies of Usher-related hearing loss make it a good candidate for gene therapy and discuss how various forms of gene therapy could be applied to Usher-related hearing loss.     

Ubiquitin-Specific Protease 29 Regulates Cdc25A-Mediated Tumorigenesis

Cell division cycle 25A (Cdc25A) is a dual-specificity phosphatase that is overexpressed in several cancer cells and promotes tumorigenesis. In normal cells, Cdc25A expression is regulated tightly, but the changes in expression patterns in cancer cells that lead to tumorigenesis are unknown. In this study, we showed that ubiquitin-specific protease 29 (USP29) stabilized Cdc25A protein expression in cancer cell lines by protecting it from ubiquitin-mediated proteasomal degradation. The presence of USP29 effectively blocked polyubiquitination of Cdc25A and extended its half-life. CRISPR-Cas9-mediated knockdown of USP29 in HeLa cells resulted in cell cycle arrest at the G0/G1 phase. We also showed that USP29 knockdown hampered Cdc25A-mediated cell proliferation, migration, and invasion of cancer cells in vitro. Moreover, NSG nude mice transplanted with USP29-depleted cells significantly reduced the size of the tumors, whereas the reconstitution of Cdc25A in USP29-depleted cells significantly increased the tumor size. Altogether, our results implied that USP29 promoted cell cycle progression and oncogenic transformation by regulating protein turnover of Cdc25A.     

基于改进SSD的变压器套管红外图像油位智能识别方法

为解决现有基于红外图像识别变压器套管油位存在的过于依赖温度信息、人工处理效率低下等问题,文中结合目标检测技术提出了一种基于改进单次检测器(SSD)的套管智能油位识别方法。通过引入SSD目标检测方法,检测红外图像中的套管区域,加入损失函数以改进SSD算法从而提高套管检测准确率,并进一步通过简单线性迭代聚类(SLIC)的应用实现了不依赖红外图像温度信息的油位检测。对比文中提出的基于红外图像的油位识别算法检测结果与人工油位检测结果,表明文中提出的算法不仅在效率上领先于传统的温度检测方式,且其误差较小,仅为0.08%。对比结果验证了所提算法在保证检测精度的情况下可大幅度提高检测效率,有效提升套管故障诊断效率和智能化水平。     

Material Design for 3D Multifunctional Hydrogel Structure Preparation

Hydrogels are recognized as one of the most promising materials for e-skin devices because of their unique applicable functionalities such as flexibility, stretchability, biocompatibility, and conductivity. Beyond the excellent sensing functionalities, the e-skin devices further need to secure a target-oriented 3D structure to be applied onto various body parts having complex 3D shapes. However, most e-skin devices are still fabricated in simple 2D film-type devices, and it is an intriguing issue to fabricate complex 3D e-skin devices resembling target body parts via 3D printing. Here, a material design guideline is provided to prepare multifunctional hydrogels and their target-oriented 3D structures based on extrusion-based 3D printing. The material design parameters to realize target-oriented 3D structures via 3D printing are systematically derived from the correlation between material design of hydrogels and their gelation characteristics, rheological properties, and 3D printing processability for extrusion-based 3D printing. Based on the suggested material design window, ion conductive self-healable hydrogels are designed and successfully applied to extrusion-based 3D printing to realize various 3D shapes.     

Room-Temperature Solid-State Lithium Metal Batteries Using Metal Organic Framework Composited Comb-Like Methoxy Poly(ethylene glycol) Acrylate Solid Polymer Electrolytes

Solid polymer electrolyte with good thermal stability and flexibility is an excellent candidate for solid-state lithium metal batteries, while its low ionic conductivity caused by high crystallinity limits its application at ambient temperature. Here a metal organic framework (zeolitic imidazolate framework-8, ZIF-8) composited comb-like methoxy poly(ethylene glycol) acrylate polymer electrolyte (MCPE) with high ionic conductivity (9.96 × 10⁻⁵ S cm⁻¹ at 30 °C) is prepared by an in situ UV polymerization method. The as-prepared MCPE exhibits improved mechanical property due to the introduction of porous ZIF-8 nanofillers, which is beneficial to suppress the growth of lithium dendrites. Consequently, the LiFePO₄||MCPE||Li cells show a high capacity of 116 mAh g⁻¹ at 30 °C and 0.5 C, and maintain 89.4% of initial capacity after 150 cycles with the average Coulombic efficiency of 99.9%. These results demonstrate that the MCPE shows great potential in solid-state lithium metal batteries near room temperature.     

Red-Blood-Cell-Membrane-Coated Metal-Drug Nanoparticles for Enhanced Chemotherapy

To overcome high toxicity, low bioavailability and poor water solubility of chemotherapeutics, a variety of drug carriers have been designed. However, most carriers are severely limited by low drug loading capacity and adverse side effects. Here, a new type of metal-drug nanoparticles (MDNs) was designed and synthesized. The MDNs self-assembled with Fe(III) ions and drug molecules through coordination, resulting in nanoparticles with high drug loading. To assist systemic delivery and prolong circulation time, the obtained MDNs were camouflaged with red blood cell (RBCs) membranes (RBCs@Fe-DOX MDNs) to improve their stability and dispersity. The RBCs@Fe-DOX MDNs presented pH-responsive release functionalities, resulting in drug release accelerated in acidic tumor microenvironments. The outstanding in vitro and in vivo antitumor therapeutic outcome was realized by RBCs@Fe-DOX MDNs. This study provides an innovative design guideline for chemotherapy and demonstrates the great capacity of nanomaterials in anticancer treatments.