Stimulation of large-conductance Ca2+-activated K+ channels by Evans blue in cultured endothelial cells of human umbilical veins

The effect of Evans blue (EB) on large-conductance Ca2+-activated K+ (BKCa) channels was investigated in cultured endothelial cells of human umbilical veins. In whole-cell configuration, EB (50 microM) reversibly increased the amplitude of K+ outward currents (IK). When the patch pipettes were filled with 10 mM EGTA, its stimulatory effect on IK was unaltered. Further application of EB in the presence of suramin, a blocker of P2-purinergic receptor, or AOPCP, an inhibitor of 5'-nucleotidase, still increased IK. However, charybdotoxin (100 nM) suppressed EB-induced increase in IK. In inside-out configuration, bath application of EB (50 microM) did not change single channel conductance but significantly increased the activity of BKCa channels. The EB-induced increase in the activity of BKCa channels was independent on internal Ca2+. EB (50 microM) shifted the activation curve of BKCa channels to less positive membrane potentials by approximately 20 mV. The change in the kinetic behavior of BKCa channels caused by EB in these cells is due to an increase in mean open time and a decrease in mean closed time. These results indicate that EB can stimulate the activity of BKCa channel in endothelial cells. This effect is unrelated to its blockade of P2-purinergic receptors or inhibition of 5'-nucleotidase. The direct stimulation of these ionic channels by EB may contribute to its effect on capillary permeability.     

Increasing Understanding of Technology Management through Challenge Mapping

The authors seek to clarify what is understood by the management of technology by involving a group of managers in a workshop to identify specifically what they believe to be their critical difficulties and challenges in managing technology. The approach is shown to be a means of enhancing creativity, designed not so much for creating good answers and solutions, but rather for discovering good questions and challenges and for conceptualizing complex issues. Virtually all of the challenges selected as most important had much more to do with leading people to think more innovatively and collaboratively than with new technology itself.     

The power of gifts: organizing social relationships in open source communities

Abstract. In writings on the open source software development model, it is often argued that it is successful as a result of the gift economy that embraces activities in online communities. However, the theoretical foundations for this argument are seldom discussed and empirically tested. Starting with the 'classic' theories of gift giving, we discuss how they need to be developed in order to explain gift-giving practices in digital domains. In this paper, we argue that the gift economy is important, not only because it creates openness, but also because it organizes relationships between people in a certain way. Open source software development relies on gift giving as a way of getting new ideas and prototypes out into circulation. This also implies that the giver gets power from giving away. This power is used as a way of guaranteeing the quality of the code. We relate this practice to how gifts, in the form of new scientific knowledge, are given to the research community, and how this is done through peer review processes.     

Filter‐ versus wrapper‐based feature selection for credit scoring

We address the problem of credit scoring as a classification and feature subset selection problem. Based on the current framework of sophisticated feature selection methods, we identify features that contain the most relevant information to distinguish good loan payers from bad loan payers. The feature selection methods are validated on several real‐world datasets with different types of classifiers. We show the advantages following from using the subspace approach to classification. We discuss many practical issues related to the applicability of feature selection methods. We show and discuss some difficulties that used to be insufficiently emphasized in standard feature selection literature. © 2005 Wiley Periodicals, Inc. Int J Int Syst 20: 985–999, 2005.     

Attentional effects on preattentive vision: Spatial precues affect the detection of simple features.

Most accounts of visual perception hold that the detection of primitive features occurs preattentively, in parallel across the visual field. Evidence that preattentive vision operates without attentional limitations comes from visual search tasks in which the detection of the presence or absence of a primitive feature is independent of the number of stimuli in a display. If the detection of primitive features occurs preattentively, in parallel and without capacity limitations, then it should not matter where attention is located in the visual field. The present study shows that even though the detection of a red element in an array of gray elements occurred in parallel without capacity limitations, the allocation of attention did have a large effect on search performance. If attention was directed to a particular region of the display and the target feature was presented elsewhere, response latencies increased. Results indicate that the classic view of preattentive vision requires revision. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Antifungal activity of lauric acid derivatives against Aspergillus niger

The antifungal effects of lauric acid and four lauric acid derivatives (monolauroylglycerol, D-laurate A, T-laurate A, 6-O-lauroysucrose) were tested on the spore germination and the growth rate of Aspergillus niger DMF 0801. The results showed that the tested substances varied in their antifungal activity and they also confirmed the relation of the structure of tested substances and their antifungal effects. Monolauroylglycerol at concentration level between 0.2 and 1.8 mmol l^-1 caused the highest inhibition of spore outgrowths whereas 6-O-lauroysucrose at concentration between 0.05 and 1.8 mmol l^-1 did not exhibit inhibition of spore germination. The inhibition of colony growth rate was detected mainly at presence of 6-O-lauroysucrose.     

Incremental Dynamics

A new system of dynamic logic is introduced and motivated, witha novel approach to variable binding for incremental interpretation. Thesystem is shown to be equivalent to first order logic and complete.The new logic combines the dynamic binding idea from DynamicPredicate Logic with De Bruijn style variable free indexing. Quantifiersbind the next available variable register; the indexing mechanismguarantees that active registers are never overwritten by newquantifiers actions. Apart from its interest in its own right, theresulting system has certain advantages over Dynamic Predicate Logic orDiscourse Representation Theory. It comes with a more well behaved(i.e., transitive) consequence relation, it gives a more explicitaccount of how anaphoric context grows as text gets processed, and ityields new insight into the dynamics of anaphoric linking in reasoning.Incremental dynamics also points to a new way of handling contextdynamically in Montague grammar.     

Convexity Rule for Shape Decomposition Based on Discrete Contour Evolution

We concentrate here on decomposition of 2D objects into meaningfulparts of visual form, orvisual parts. It is a simple observation that convex parts of objects determine visual parts. However, the problem is that many significant visual parts are not convex, since a visual part may have concavities. We solve this problem by identifying convex parts at different stages of a proposed contour evolution method in which significant visual parts will become convex object parts at higher stages of the evolution. We obtain a novel rule for decomposition of 2D objects into visual parts, called the hierarchical convexity rule, which states that visual parts are enclosed by maximal convex (with respect to the object) boundary arcs at different stages of the contour evolution. This rule determines not only parts of boundary curves but directly the visual parts of objects. Moreover, the stages of the evolution hierarchy induce a hierarchical structure of the visual parts. The more advanced the stage of contour evolution, the more significant is the shape contribution of the obtained visual parts.     

Real-Time Fetal ECG Recording

We present a real-time abdominal recording method for the FECG based on a minimization procedure for the MECG interference, by weighted addition of four abdominal leads containing FECG with a large amount of MECG. The minimization of the MECG does not influence the FECG recording adversely. FECG complexes coinciding with MECG complexes are also recorded. Use is made of programmable amplifiers that multiply analog abdominal leads by digital coefficients. These coefficients are calculated at the beginning of the recording by a microcomputer system. Experimental results are shown that clarify the advantages of the measurement principle. The real-time recorded FECG is wide-banded, so that all generally known ECG signal processing techniques, such as complex averaging, can be performed.