Natural Ligands of Porcine Olfactory Binding Proteins

Knowledge of endogenous ligands of olfactory binding proteins is a prerequisite for studying their role in odor and pheromone transduction. Here, we report the extraction, derivatization, and characterization by gas chromatography-mass spectrometry of the natural ligands of pig, Sus scrofa (L.), Von Ebner’s Gland protein (VEG) and odorant binding protein (OBP). We identified two isoforms (VEG1 and VEG2), which differed only by the linkage of an O-N-acetylglucosamine (O-GlcNac) group on VEG1. The natural ligands of VEG1 were characterized as two isomers of testosterone, whereas ligands of VEG2 and OBP were fatty acids or their derivatives. Our findings suggest that the binding specificity of VEG1 for steroids is governed by the presence of an O-GlcNac moiety on the protein. This specificity was confirmed by the binding of radiolabeled testosterone only by VEG1 in an in-gel binding assay. This is the first evidence for a post-translational modification in the process of odorant discrimination by olfactory binding proteins.     

Immunocytochemical localisation of para-coumaric acid and feruloyl-arabinose in the cell walls of maize stem

Two phenolic compounds, p-coumaric acid and feruloyl-arabinose, were localised by immunocytochemistry in the cell walls of the apical internode of two lines of maize (Co125 and W401) of different digestibility. The compounds were detected at two stages of cell maturity in the lignified tissues (sclerenchyma, fibres and xylem) and in the medullary parenchyma, which, in the samples studied, was not lignified. p-Coumaric acid is a phenolic acid associated with lignins, which confer resistance on plant cell walls to microbial degradation in the rumen. Feruloyl-arabinose is a compound associated with xylans, the principal hemicelluloses in Gramineae, which are potentially degradable. Labelling of p-coumaric acid decreased in both maize lines with cell age and as the cell walls became lignified. The mass of lignin deposited in the cell walls masked p-coumaric acid, thereby making it less accessible to the antibodies. There was an inverse relationship in the labelling of p-coumaric acid and feruloyl-arabinose. Feruloyl-arabinose was more heavily labelled as the plant cell walls matured in all the lignified tissues of both maize lines and in the parenchyma of the less digestible line. All tissues except the parenchyma were more heavily labelled with both sera in Co125, the more digestible line. © 1998 Society of Chemical Industry.     

Fermentation by Amylolytic Lactic Acid Bacteria and Consequences for Starch Digestibility of Plantain, Breadfruit, and Sweet Potato Flours

The potential of tropical starchy plants such as plantain (Musa paradisiaca), breadfruit (Artocarpus communis), and sweet potato (Ipomoea batatas) for the development of new fermented foods was investigated by exploiting the capacity of some lactic acid bacteria to hydrolyze starch. The amylolytic lactic acid bacteria (ALAB) Lactobacillus plantarum A6 and Lactobacillus fermentum Ogi E1 were able to change the consistency of thick sticky gelatinized slurries of these starchy fruits and tubers into semiliquid to liquid products. Consequently, a decrease in apparent viscosity and an increase in Bostwick flow were observed. These changes and the production of maltooligosaccharides confirmed starch hydrolysis. Sucrose in sweet potato was not fermented by strain A6 and poorly fermented by strain Ogi E1, suggesting possible inhibition of sucrose fermentation. In all 3 starchy plants, rapidly digestible starch (RDS) was higher than slowly digestible starch (SDS) and resistant starch (RS) represented between 17% and 30% dry matter (DM). The digestibility of plantain was not affected by fermentation, whereas the RDS content of breadfruit and sweet potato decreased and the RS content increased after fermentation. Practical Application: The characteristics resulting from different combinations of gluten free starchy plants (plantain, breadfruit, sweet potato) and amylolytic lactic acid bacteria (ALAB) offer opportunities to develop new functional fermented beverages, mainly for breadfruit and sweet potato, after further investigation of their formulation, sensory attributes, nutritional, and prebiotic characteristics.     

Water quality assessment of a recently refilled reservoir: The case of Bütgenbach Reservoir, Belgium

Bütgenbach Reservoir is situated in the High Ardennes plateau in eastern Belgium (50°25′N; 6°13′E). It is used principally for flood control and for production of hydroelectric energy. It has been subjected to a previous series of studies because of its eutrophication problems and their impacts on the local economy. Bütgenbach Reservoir was emptied during spring 2004 for dam restoration, being refilled in mid‐September of the same year. Selected physicochemical and biological parameters (temperature, dissolved oxygen, pH, inorganic and organic nutrients, chlorophyll‐a) were measured fortnightly during the lake's productive season (mid‐April to mid‐October 2005) at three sampling sites throughout the water column. The water quality conditions after refilling of the lake were compared to previous studies accomplished at the same sampling sites (prior to emptying the lake). The actual trophic status was mesotrophic to eutrophic, based on the combination of total phosphorous and chlorophyll‐a concentrations, as well as water transparency. Bütgenbach Reservoir generally exhibits good water quality, based on the French water quality system SEQ‐eau. A longitudinal decrease in water quality was observed from upstream to downstream, because mainly of the differences in lake bottom morphology and water residence time, and their impacts on nutrient distribution in the lake.     

A Functional Programming Framework for Latency Insensitive Protocol Validation

Latency insensitive protocols (LIPs) have been proposed as a viable means to connect synchronous IP blocks via long interconnects in a system-on-chip. The reason why one needs to implement LIPs on long interconnects stems from the fact that with increasing clock frequencies, the signal delay on some interconnects exceeds the clock period. Correctness of a system composed of synchronous blocks communicating via LIPs is established by showing latency equivalence between a completely synchronous composition of the blocks, and the LIP based composition. A design flow based on a synchronous composition specification, and stepwise refinement to LIP composition can be easily conceived, and a proof obligation to show latency equivalence between the synchronous specification and the refinement needs to be discharged. In this work, we propose a functional programming based framework for modeling and simulating LIP, and implement the semantics of various refinement steps in the programming model, so we can validate the LIP model against the original system within this functional programming framework. Such validation becomes easier due to the inherent denotational model of functional languages. We specifically use Standard ML to model the original system implementation as well as its latency insensitive version and compare the two by creating a model that contains both, giving them the same inputs and checking their outputs to be latency equivalent.     

Réflexion libre à travers ľATM paragraphs on ATM

Fifteen years after its first tentative steps in the laboratory, ATM seems to have reached the thankless age of confrontation with the reality of operational networks while others would say the age of potential to fulfil and promises to keep. The following article does not pretend to be an in-depth technical analysis of a particular aspect or development of ATM but rather the thoughts of a witness to a number of past, present and future issues. The objective is much more to stimulate thought on ATM than to provide definitive answers to often difficult questions.     

ATM: A contribution to the debate on broadband ISDN

The definition of broadband ISDN recently became a major topic of research and predevelopments. Fast packet technologies are widely recognized as the basis for BISDN. Even if international consensus has appeared far more quickly than could have been expected a few years ago, a lot of issues remain open. This paper is a contribution to discussions on some important topics relating to the definition of asynchronous transfer mode.     

Application du concept d'echelles multiples a l'ecoulement dans une turbomachine axiale

An asymptotic theory to the flow in an axial compressor is presented with the aim of devising a coupling process between the so-called meridian through flow and the flow around cascades. The small parameter ? is the inverse of the (supposed ? 1) number of blades per row and/or number of stages. As a matter of fact, the cascade flow is treated as a small perturbation of the through flow and has to be computed, locally, as the two dimensional unsteady flow around an array of couples of cascades alternately fixed and in motion. The array is constructed by developing on a plane the section of the compressor by a circular cylinder, and continuing by periodicity, the couple of cascades, so obtained, at each location. The coupling between through flow and cascade flow is part of the analysis. It occurs by the way that the equations of through flow are obtained through an averaging process, completed on a domain of periodicity of the array of cascades, while the through flow appears, locally, as an unperturbed flow for the linearized problem defining the cascade flow. The tridimensional nature of the complete flow is built in by the coupling itself, as is visualized by the occurence of source terms in each of the two sets of equations describing through flow and cascade flow. This paper is aimed at giving a preliminary answer to the question of how to devise an, as rational as possible, way of inscribing the familiar scheme of cascade flow within the computation of a mean through flow. The main output is that the concept of cascade flow should be revisited and reassessed as one of unsteady flow around an array of cascades.     

The characterization of porous electrodes by impedance measurements

Impedances of a gold-powder and a Raney-gold electrode were measured over a wide frequency range, and were found to be very similar to those of cylindrical pore of finite depth. The simulation calculation for sphere-packed electrode impedance was performed. It was observed that even though each sphere-layer shows the impedance related to an occluded pore-shape, the overall impedance is similar to that of a cylindrical pore electrode. When the penetration depth approaches the pore depth, the shape of the pore wall has little influence on the impedance. Thus, impedance measurement techniques can be applied to porous electrodes of more intricate pore-texture, and evaluate the radius, depth and pore number of its equivalent cylindrical pore electrode. These values determined for a Raney-gold electrode are in very good agreement with those determined by other methods. The case of a more realistic fuel cell electrode such as Raney-nickel with a metal-electrolyte-gas system, was also tentatively examined.