一种5 重real-time PCR筛查转基因水稻方法的建立

以转基因水稻中最常用的CaMV35S启动子、NOS终止子、Cry1Ab/Ac基因、HPT基因及SPS水稻内标基因为研究对象，利用5 种不同的荧光信号（FAM、HEX、Taxas Red、Cy5、Cy5.5）进行多重实时聚合酶链式反应（real-time polymerase chain reaction，real-time PCR）检测方法的研究。通过引物组合筛选、反应体系优化、特异性测试、灵敏度测试、适用性测试等一系列实验，建立了5 重real-time PCR方法，灵敏度可达0.032%。此方法具有灵敏度高、结果准确、通量大等优点，可实现水稻中转基因成分的快速、高效检测。     

瞬变电磁法不同工作装置在强干扰区的综合应用

目前瞬变电磁法勘探广泛运用在煤矿系统,主要解决地下水的问题.随着科技的发展和进步,瞬变电磁法勘探仪器大致分2类,国外仪器一般采用大功率、大线框的大定源框内回装置;国内仪器一般采用小功率小线圈、低电压、高电流的重叠回线装置.在作业过程中,瞬变电磁法发射线框与接收设备受电磁干扰较为严重,不同的工作装置优势不同,抗干扰能力不同.通过对山西潞安集团李村矿的工作研究,证明了采用不同工作装置在高压线附近进行勘探,可起到互补的作用,达到勘探的目的 .     

Lead-free sodium niobate nanowires with strong piezo-catalysis for dye wastewater degradation

In this work, the hydrothermally-synthesized sodium niobate nanowires were used to decompose Rhodamine B dye solution through the piezo-catalytic effect. With the sodium niobate catalyst, a high piezo-catalytic degradation ratio of ～80% was achieved under the excitation of vibration for the Rhodamine B dye solution (～5?mg/l). These active species in the catalytic process, hydroxyl radicals and superoxide radicals with the strong oxidation ability, were also observed, which confirmed the key role of piezoelectric effect for piezo-catalysis. The piezo-catalysis of sodium niobate nanowires provides a high-efficiency and reusable tool in application in depredating the dye wastewater.     

郭庄煤业选煤厂振动筛筛选形式的优化研究

针对郭庄煤业选煤厂振动筛振动筛分方式可靠性差、筛分效率低下、物料筛分率低的难题,本文提出了三种新的振动筛分方案,对不同筛分方案的实际应用效果进行了分析。结果表明三移动一摆动的振动筛分方式对物料的综合分散度最高,稳定性好,该筛分方案已在郭庄煤业选煤厂煤炭筛分过程中得到了全面的应用。     

Effects of hydrolysis of precursor on the structures and properties of polymer-derived SiBN ceramic fibers

In this paper, polyborosilazane precursor was synthesied from HMDZ, HSiCl₃, BCl₃ and CH₃NH₂ using a multistep method. By controlling the storage conditions, parts of the polyborosilazane fibers were hydrolyzed. FT-IR, NMR, XRD, TEM and monofilament tensile strength test were employed to study the effects of hydrolysis of precursor on the structures and properties of polymer-derived SiBN ceramic fibers. FT-IR and NMR results indicate that Si-N group in PBSZ reacts with H₂O to form Si-O-Si group. After pyrolysis reaction at 1400℃, Si-O-Si group will finally transformed into highly ordered cristobalite and β-quartz, resulting in formation of the wrinkled surface of the obtained SiBN ceramic fiber. The strip-like defects on fiber surface, according to monofilament tensile strength test, had a significant effect on mechanical property of the obtained SiBN ceramic fiber and caused no increase in fiber tensile strength of hydrolytic polyborosilazane fiber before and after pyrolytic process.     

平煤六矿大埋深高瓦斯切顶留巷技术应用与实践#br#

为保证矿井安全高效生产，针对平煤股份公司六矿戊8 32010工作面机巷平均埋深700 m、瓦斯原始含量5.12 m3/t，原始瓦斯压力0.52 MPa的情况，开展卸压切顶留巷工作。通过对留巷全过程矿压监测，分析成巷矿压分布规律，将留巷分为动压剧烈影响区、动压缓慢影响区和成巷稳定区三部分，矿压分别来源于超前支承压力和留巷内顶板短悬臂梁失稳。留巷进入稳定区后，随着巷道超前支护液压支架等支护设施的撤除，巷道会出现二次稳定过程，主要源于顶板对于巷道超前支护液压支架工作阻力的再平衡。切顶留巷技术提高了工作效率，很好地解决了矿井采掘接替紧张的问题。     

Process monitoring method based on correlation variable classification and vine copula

Chemical processes are becoming increasingly complicated, leading to an increase in process variables and more complex relationships among them. The vine copula has a significant advantage in portraying the dependence of high-dimensional variables. However, as the dimensions increase, the vine copula model incurs a high computational load; such pressure greatly reduces model efficiency. Relationships among variables in the industrial process are complex. Different variables may be strongly or weakly associated or even independent. This paper proposes a process monitoring method based on correlation variable classification and vine copula. The weighted correlation measure is first used to divide variables into a correlated subspace and weakly correlated subspace. Then, two vine structures, C-vine and D-vine, are applied to the correlated and weakly correlated subspaces, respectively. This method takes advantage of C-vine for correlated variables and the flexibility of D-vine for weakly correlated variables. Finally, comprehensive statistics are established based on different subspaces. Monitoring results of the numerical system and the Tennessee Eastman process demonstrate the effectiveness and validity of the proposed method.