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201.
Blanc F Bernard H Alessandri S Bublin M Paty E Leung SA Patient KA Wal JM 《Molecular nutrition & food research》2008,52(Z2):S166-S175
Highly purified allergens namely cow's milk alpha-lactalbumin (ALA). (Bos d 4), beta-lactoglobulin (BLG) (Bos d 5) and casein (Bos d 8) and goat's milk casein were prepared from the raw milk from a single animal with a known genetic background. Consequently the natural isoforms are limited, constant and characterized. Purification included selective precipitations and chromatographical steps. Characterization of structure and allergenic activity assessment of milk allergens were carried out using physicochemical and immunochemical methods. Taken together data demonstrated the absence of impurities and of contamination by other milk allergens in each preparation. NMR and circular dichroism analyses confirmed the native conformation and proper folding of ALA and BLG and the expected absence of folding of bovine and caprine casein. Enzyme immuno assays confirmed the native conformation of BLG and the purity and immunoreactivity of all the proteins. The allergenic activity, e. g. the IgE binding capacity, of purified proteins was identical as that of those proteins when present in milk. The purified proteins also demonstrated the ability to provoke the degranulation of humanized rat basophilic leukaemia cells. All the data thus confirm the purity, identity, structural conformation and functionality of the prepared milk allergens. 相似文献
202.
MPPA光动力作用诱导人鼻咽癌细胞凋亡的实验研究 总被引:3,自引:0,他引:3
为观察MPPa光动力作用对鼻咽癌细胞凋亡的影响,应用AnnexinV—PI双染结合流式细胞仪分析MPPa光动力作用后人鼻咽癌细胞株CNE2细胞发生凋亡和继发性坏死的比率。结果显示MPPa光动力作用实验组人鼻咽癌细胞株CNE2细胞发生凋亡和继发性坏死的比率分别增加到16.43 %和4.64 % ,且均显著高于单纯光照射组、单纯MMPa光敏剂处理组和假照射组(P <0 .0 1) ,而三对照组间无明显差异(P >0 .0 5 )。表明MPPa光动力作用能有效诱导低分化人鼻咽癌细胞株CNE2细胞凋亡的发生。这也可能是MPPa光动力作用杀伤鼻咽癌的重要机制之一。 相似文献
203.
Xi Li Shanzhi Chen Dan Chen Hong Ji Victor C. M. Leung 《International Journal of Communication Systems》2013,26(4):530-548
With the introduction of low‐powered pico/femto‐base stations and relay nodes into the macro‐cell, recent heterogeneous networks provide an attractive approach for future wireless communication. Although it may achieve better coverage and higher capacity, several problems remain unsolved before practical deployment. For example, how to select the proper cell from neighbor low‐powered cells and then occupy the radio resource without interference on macro‐users is both important and challenging, especially for rigorous multimedia applications. The traditional cell access algorithms and quality‐control parameters such as delay or throughput no longer suit well in this complex environment. An effective approach should be pursued. In this paper, we investigate this interesting cell association problem and propose a complete green resolution on the basis of thorough discussions about the multimedia transmission under these concerns. Cognitive radio is introduced to share spectrum between macro‐cell and low‐powered cells while securing the transmission of authorized macro‐users. We also bring forth the concept of ‘interference balance’ to better manage the overall interference and energy consumption in the network. Restless bandit model is formulated on the basis of channel state, data rate, interference control, and the carefully chosen intra‐refreshing rate for multimedia traffic. Then the cell association scheme is designed to be efficient and practical because of the simple index property of our model output. Simulation results have proven the performance of our proposed resolution compared with existing algorithms on interference constraint, multimedia distortion, and overall network energy consumption balance. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
204.
Jin Xuan Dennis Y.C. Leung Michael K.H. Leung Meng Ni Huizhi Wang 《International Journal of Hydrogen Energy》2012
Microchannel reformer integrated with H2 selective membrane offers an efficient, compact and portable way to produce hydrogen. The performance of a membrane-based microfluidic reformer is restricted by species diffusion limitation within the porous support of the membrane. Recent development in novel catalytic-supported membranes has the potential to enhance H2 production by decimating the diffusion limitation. Loading a Pd-Ag layer on to a Ni-catalytic porous support, the membrane achieves both H2 separation and production functions. In this study, a two-dimensional CFD model combined with chemical kinetics has been developed to simulate a microchannel autothermal reformer fed by methane. The species conversion and transport behaviors have been studied. The results show that the permeation process enhances the mass transport within the catalytic layer, and as a result, the reactions are intensified. Most notably, the effectiveness factor of the water-gas shift reaction as high as 6 is obtained. In addition, the effects of gaseous hourly space velocity (GHSV) on methane conversion and H2 flux through the membrane are also discussed, and an optimal value of GHSV is suggested. 相似文献
205.
206.
Chatterjee Saikat Li Donghui Leung Jackie Sengupta Joydeep Chattopadhyay Kinnor 《Metallurgical and Materials Transactions B》2017,48(2):1035-1044
Metallurgical and Materials Transactions B - Inert gas shrouding in tundish can result in the formation of a tundish open eye (TOE) due to the presence of reversed flows on the upper surface of the... 相似文献
207.
J. Benziger L. Cadonati F. Calaprice M. Chen A. Corsi F. Dalnoki-Veress R. Fernholz R. Ford C. Galbiati A. Goretti E. Harding Aldo Ianni Andrea Ianni S. Kidner M. Leung F. Loeser K. McCarty D. McKinsey A. Nelson A. Pocar C. Salvo D. Schimizzi T. Shutt A. Sonnenschein 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》2009,608(3):464-474
The system for controlled filling of the nested flexible scintillator containment vessels in the Borexino solar neutrino detector is described. The design and operation principles of pressure and shape monitoring systems are presented for gas filling, gas displacement by water, and water displacement by scintillator. System specifications for safety against overstressing the flexible nylon vessels are defined as well as leak-tightness and cleanliness requirements. The fluid-filling system was a major engineering challenge for the Borexino detector. 相似文献
208.
209.
An experimental study was performed to investigate the effects of the nozzle length on the air-pollutant-emission and noise-radiation behaviors of a burner utilizing a multi-fuel-jet inverse diffusion flame (MIDF). Comparison of the experimental results obtained from two MIDF burners, one with a long nozzle and the other with a short nozzle, operating under the same air/fuel supply conditions (Reair and Ф) shows rather significant differences in the flame appearance, flame centerline temperature, CO/CO2 concentrations and the noise radiation. The nozzle length influences development of the jets and hence interaction between the air/fuel jets including their mixing process. The short nozzle produces a flame with a shorter base height and a smaller potential core due to the enhanced air/fuel mixing. It also leads to faster and more complete combustion at the inner reaction cone of the flame due to the stronger and faster air/fuel mixing. The nozzle length affects the CO and CO2 concentrations, and higher peak values are obtained with the short-nozzle flame. Flame noise of the MIDF is defined as the noise radiation at different flame heights, which is of varying strength but of the same dominant frequency in the range of 250–700 Hz. The noise radiation from the inner reaction cone of the flame is stronger than that from the lower and upper parts of the flame, and the maximum noise radiation occurs when the total amounts of air and fuel in the combustion zone are at the stoichiometric air/fuel ratio. For all the experiments conducted in the present study, the MIDF produced by the long nozzle is always noisier than its counterpart and it is due to the increase of the low-frequency noise components. 相似文献
210.
Kit Nam Francis Leung Yuan Lin ZhangKin Keung Lai 《Reliability Engineering & System Safety》2011,96(11):1542-1551
In this paper, a cold standby repairable system consisting of two dissimilar components and one repairman is studied. Assume that working time distributions and repair time distributions of the two components are both exponential, and Component 1 has repair priority when both components are broken down. After repair, Component 1 follows a geometric process repair while Component 2 obeys a perfect repair. Under these assumptions, using the perfect repair model, the geometric process repair model and the supplementary variable technique, we not only study some important reliability indices, but also consider a replacement policy T, under which the system is replaced when the working age of Component 1 reaches T. Our problem is to determine an optimal policy T? such that the long-run average loss per unit time (i.e. average loss rate) of the system is minimized. The explicit expression for the average loss rate of the system is derived, and the corresponding optimal replacement policy T? can be found numerically. Finally, a numerical example for replacement policy T is given to illustrate some theoretical results and the model's applicability. 相似文献