Response of normal aorta to endovascular grafting: a serial histopathological study

The initial data on the effect of ruthenium red on mature human type-1 VDAC are presented. Highly enriched human type-1 porin in planar lipid bilayers shows lowered voltage-dependence whenever a commercially available ruthenium red preparation is applied. The hexavalent polycationic dye ruthenium red affects different functions in varying cell compartments. Concerning the plasma membrane of cells the actual data, together with our former measurements on the interaction of VDAC and the polycationic synthetic polyamine Compound 48/80, refer to a second VDAC opener, which is relevant for studies on the stimulation of exocytotic processes of different cell types.     

Recovery of biosurfactants by ultrafiltration

Ultrafiltration was used in a one-step method to purify and concentrate biosurfactants—surfactin and rhamnolipids—from culture supernatant fluids. The ability of surfactant molecules to form micelles at concentrations above the critical micelle concentration allows these aggregates to be retained by relatively high molecular weight cut-off membranes. Lower molecular weight impurities such as salts, free amino acids, peptides and small proteins are easily removed. Various molecular weight cut-off membranes were examined for the retention of surfactin and rhamnolipids (mol. wts 1036 and 802 respectively). Amicon XM 50 was the superior membrane for retention of surfactin and a 160-fold purification was rapidly achieved. The YM 10 membrane was the most appropriate for rhamnolipid recovery. Ultrafiltration can play an important role in biosurfactant purification as large volumes of media can be processed rapidly at extremely low cost.     

Bioactivity of autologous irradiated renal cell carcinoma vaccines generated by ex vivo granulocyte-macrophage colony-stimulating factor gene transfer

Granulocyte-macrophage colony-stimulating factor (GM-CSF) gene-transduced, irradiated tumor vaccines induce potent, T-cell-mediated antitumor immune responses in preclinical models. We report the initial results of a Phase I trial evaluating this strategy for safety and the induction of immune responses in patients with metastatic renal cell carcinoma (RCC). Patients were treated in a randomized, double-blind dose-escalation study with equivalent doses of autologous, irradiated RCC vaccine cells with or without ex vivo human GM-CSF gene transfer. The replication-defective retroviral vector MFG was used for GM-CSF gene transfer. No dose-limiting toxicities were encountered in 16 fully evaluable patients. GM-CSF gene-transduced vaccines were equivalent in toxicity to nontransduced vaccines up to the feasible limits of autologous tumor vaccine yield. No evidence of autoimmune disease was observed. Biopsies of intradermal sites of injection with GM-CSF gene-transduced vaccines contained distinctive macrophage, dendritic cell, eosinophil, neutrophil, and T-cell infiltrates similar to those observed in preclinical models of efficacy. Histological analysis of delayed-type hypersensitivity responses in patients vaccinated with GM-CSF-transduced vaccines demonstrated an intense eosinophil infiltrate that was not observed in patients who received nontransduced vaccines. An objective partial response was observed in a patient treated with GM-CSF gene-transduced vaccine who displayed the largest delayed-type hypersensitivity conversion. No replication-competent retrovirus was detected in vaccinated patients. This Phase I study demonstrated the feasibility, safety, and bioactivity of an autologous GM-CSF gene-transduced tumor vaccine for RCC patients.     

The central position of education in knowledge mobilization: insights from network analyses of spatial reasoning research across disciplines

Scientometrics - Knowledge mobilization is becoming increasingly important for research collaborations, but few methodologies support increased knowledge sharing. This study provides insights,...     

Atmospheric pressure ionization in a miniature mass spectrometer

A miniature cylindrical ion trap mass spectrometer featuring an atmospheric pressure interface allowing atmospheric pressure chemical ionization and electrospray ionization is described together with its analytical performance characteristics. The vacuum system, ion optics, mass analyzer, control electronics system, and detection system have all been designed and built in-house. The design is based upon a three-stage, differentially pumped vacuum system with the instrument capable of being interfaced to many types of atmospheric pressure ionization sources. Ions are transferred through home-built ion optics, and instrument control is achieved through custom-designed electronics and LabView control software. Corona discharge ionization and electrospray ionization sources are implemented and used to allow the analysis of both gaseous- and solution-phase samples during the characterization of the instrument. An upper mass/charge limit of approximately 450 Th with unit resolution was achieved using a 2.5-mm-internal radius cylindrical ion trap as the mass analyzer. The specificity of the instrument can be increased by employing the MS/MS capabilities of the ion trap and has been demonstrated for nitrobenzene. Limits of detection for the trace analysis in air of the chemical warfare agent simulant methyl salicylate (1.24 ppb) and for nitrobenzene (629 pptr) are achieved. The dynamic range of the instrument is currently limited to approximately 2 orders of magnitude by saturation of the detection electronics. Isolation and collision-induced dissociation efficiencies in MS/MS experiments both greater than 50% are reported. Electrospray/nanospray data are presented on solutions including 100 microM (D,L)-arginine, 10 microM (-)-ephedrine, and 10 microM lomefloxacin.     

Combining cognitive testing and informant report to increase accuracy in screening for dementia

OBJECTIVE: Cognitive testing and an informant report questionnaire were combined to determine whether their use in combination could improve accuracy in screening for the diagnosis of dementia over either test used alone. Methods of combining test scores that can be readily applied in clinical settings were developed and assessed. METHOD: The subjects were 106 patients admitted to the geriatric hospital or outpatients assessed at the memory clinic of the university hospital system in Geneva, Switzerland. The instruments used were the Mini-Mental State and the short form of the Informant Questionnaire on Cognitive Decline in the Elderly. The diagnosis of dementia was made according to DSM-IV criteria. RESULTS: Logistic regression demonstrated that the combination of the Mini-Mental State and the Informant Questionnaire on Cognitive Decline in the Elderly resulted in more accurate prediction of caseness than either test alone. The performance of logical "or" and "and" combinations of test results and a weighted sum of scores on the two tests as screens for dementia were investigated by using receiver operating characteristic analysis. By using suitable cutoff points, both the "or" rule and the weighted sum were shown to be capable of improving performance over that of either test used alone. CONCLUSIONS: This study shows that informant report can be formally incorporated into assessment for dementia in such a way as to increase the accuracy of detection of cases and noncases. A graphical method was developed that enables the most robust approach to be applied to individual cases without any calculation.     

Water vortex hydropower technology: a state-of-the-art review of developmental trends

With the explosive growth of global energy demand coupled with effects of climate change, there is a significant shift towards green energy generation in recent years. Of the various renewable energy resources available, micro-hydro-power and pico-hydro-power remain very popular in both developed and developing countries. Since 2006, significant growth has spurted in the use of artificial free-surface vortices to generate low and ultra-low-head hydropower following the development of the so-called gravitational water vortex hydropower plant. The technology works on the principle of harnessing hydroelectric power from the high angular velocity experienced in the core of a whirlpool generated in a vortex chamber. In this article, a state-of-the-art review is undertaken on the vortex hydropower technology including a historical review of the technology, the underlying hydraulic principles of such devices, overview of research and technologies that have been deployed to date together with an evaluation of their performance and key findings. Currently, there are 19–22 known live vortex hydropower technologies operating internationally with key academic and commercial research activity in Europe and Asia. The average efficiency from these sites was found to be in the region of 53% which is lower than conventional propeller turbines but higher than waterwheel systems. It was found that the vortex plant, due to its ability to sustain relatively high efficiencies at low heads and small to medium flow rates, addresses a gap in the current turbine application chart. Its key advantage lies in the high-power densities produced compared with conventional technologies. The system also demonstrates potential to be able to function as a fish passage; however, stronger validation is required to prove this for a range of turbine systems. Finally, the authors propose a number of areas that should be investigated that should provide immediate improvements to the turbine in terms of performance.     

THE ELECTROLYSIS OF SODA-LIME GLASS—PART II1,2

The electrical resistance of soda-lime glass is markedly increased by suitable heat treatments. An investigation has been carried out upon the effect of the time of annealing on the resistance and the temperature coefficients of the resistances of annealed and unannealed glass. Migration experiments indicate that the major factor in the increasing of the resistance by annealing is probably a decrease in the speed of the carriers. The apparent resistance of glass is also a function of the extent to which the glass has been electrolyzed. In unaltered glass the conduction is in accord with Ohm's Law but with glass that has been electrolyzed under certain conditions the apparent resistance is a function of the applied voltage.