Injection techniques to anesthetize the difficult tooth

Failure to achieve anesthesia can be a significant problem in the day-to-day practice of dentistry. The usual strategy following an anesthetic failure is to reinject. Therefore, a good understanding of conventional anesthetic techniques is important. But the practitioner also needs to have a broad armamentarium of injection strategies available for the "difficult-to-anesthetize cases". These strategies include the use of 5 percent lidocaine, intrapulpal injection, periodontal ligament injection and intraosseous injection. This paper will be a brief discussion of those techniques with an emphasis on the intraosseous injection.     

Development of categorical representations for above and below spatial relations in 3- to 7-month-old infants.

A series of experiments examined the abilities of infants to form categorical representations for the spatial relations above and below. Exp 1 provided evidence that 3–4 mo olds can form categorical representations for above and below when a diamond shape was presented above or below a horizontal bar. Exps 2 and 3 showed that 3- and 4-mo olds did not form categorical representations for above and below when a number of discriminably different shapes (e.g., a diamond, a triangle, a dot) appeared above or below the bar. These more abstract categorical representations for above and below were formed by 6- to 7-mo olds (Exp 4). The findings suggest an experientially or maturationally based trend, from concrete to abstract, in the categorical representation of common spatial relations. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

X-ray topography for fractography of single-crystal components

X-ray diffraction topography is a new tool that may help fractographic analysis of single-crystal structural materials. It is sensitive to local strain and/or crystallographic orientation and provides a unique view of single-crystal samples both before and after fracture. It can find strength-and performance-limiting surface and subsurface flaws that are undetectable by other methods or are detectable only with great difficulty and provides a complementary view of the fracture surface. The attributes of synchrotron-based X-ray topography as applied to fractography are described and illustrated with examples from recent experiments on sapphire.     

Cover Picture: A Single Polymer Nanowire Photodetector (Adv. Mater. 18/2006)

Redmond and co‐workers report on p. 2379 the successful application of solution‐assisted template wetting to the high‐yield controlled synthesis of poly[(9,9‐dioctylfluorenyl‐2,7‐diyl)‐co‐(bithiophene)] (F8T2) nanowires (main picture and lower inset). Photoconductivity measurements (schematic in center) yield single‐nanowire device responsivities of ca. 0.4 mA W^–1 and external quantum efficiencies of ca. 0.1 % under monochromatic illumination, comparable with data reported for single inorganic nanowire devices.     

Isomeric Carborane Neuromuscular Blocking Agents

We synthesized a family of neuromuscular blocking agents (NMB) based on decamethonium, but containing a carborane cluster in the methylene chain between the two quaternary ammonium groups. The carborane cluster isomers o-NMB, m-NMB, and p-NMB were tested in animals for neuromuscular block and compared with agents used clinically: rocuronium and decamethonium. All three isomers caused reversible muscle weakness in mice as determined by grip strength and inverted screen tests, with a potency rank of p-NMB > rocuronium > decamethonium > m-NMB > o-NMB. The mechanism of action of the compounds was determined by using the in vitro rat phrenic nerve hemi-diaphragm preparation and electrophysiologic measurements in cells. Neostigmine reversed hemi-diaphragm weakness caused by the three isomers and rocuronium, but not succinylcholine. In electrophysiologic recordings of currents through acetylcholine receptor channels, the carborane compounds did not activate channel activity but did inhibit channel activation by acetylcholine. These results demonstrate that the carborane neuromuscular blocking agents are non-depolarizers in contrast to the depolarizing action of the parent compound.     

Predictors of summertime heat index levels in New York City apartments

During heat waves, fatal overexposure to heat most often occurs at home. It is not known how factors such as building size, floor level, and different types of air conditioning (AC) contribute to excess indoor heat. We monitored indoor temperature and humidity in 36 apartments in New York City during summers 2014 and 2015 and used these values to calculate the indoor heat index (HI). We investigated the role of AC type and building‐level factors on indoor HI using multilevel regression models. Thirty‐four of 36 homes had AC. Central and ductless AC types were associated with the coolest indoor conditions; homes with window and portable AC were significantly warmer. Apartments on the top floor of a building were significantly hotter during heat advisory periods than other apartments regardless of the presence of AC. High indoor HI levels persisted in some homes for approximately 1 day following the end of the two heat advisory periods. We provide concrete evidence of higher heat levels in top floor apartments and in homes with certain types of AC. High heat levels that persist indoors after outdoor heat has subsided may present an underappreciated public health risk.     

Edge Chipping Resistance of Alumina/Zirconia Laminates

The edge chipping test was used to measure the fracture resistance of alumina/alumina‐zirconia laminated structures. Tailored, symmetrical laminated structures were prepared with a variety of layer thickness. The laminates had a significantly greater edge chipping resistance. Laminates with thin layers were just as effective in impeding edge chips as laminates with thick layers.     

An Atomistic Understanding of Allosteric Inhibition of Glutamate Racemase: a Dampening of Native Activation Dynamics

Glutamate racemases (GR) are members of the family of bacterial enzymes known as cofactor-independent racemases and epimerases and catalyze the stereoinversion of glutamate. D-amino acids are universally important for the proper construction of viable bacterial cell walls, and thus have been repeatedly validated as attractive targets for novel antimicrobial drug design. Significant aspects of the mechanism of this challenging stereoinversion remain unknown. The current study employs a combination of MD and QM/MM computational approaches to show that the GR from H. pylori must proceed via a pre-activation step, which is dependent on the enzyme's flexibility. This mechanism is starkly different from previously proposed mechanisms. These findings have immediate pharmaceutical relevance, as the H. pylori GR enzyme is a very attractive allosteric drug target. The results presented in this study offer a distinctly novel understanding of how AstraZeneca's lead series of inhibitors cripple the H. pylori GR's native motions, via prevention of this critical chemical pre-activation step. Our experimental studies, using SPR, fluorescence and NMR WaterLOGSY, show that H. pylori GR is not inhibited by the uncompetitive mechanism originally put forward by Lundqvist et al.. The current study supports a deep connection between native enzyme motions and chemical reactivity, which has strong relevance to the field of allosteric drug discovery.