Inactivation of Escherichia coli O157:H7 on surface-uninjured and -injured green pepper (Capsicum annuum L.) by chlorine dioxide gas as demonstrated by confocal laser scanning microscopy

Cells of Escherichia coli O157:H7 on uninjured and injured surfaces of green pepper were inactivated by 0·15–1·2 mg l⁻¹ClO₂gas treatments. A membrane-surface-plating method was used for resuscitation and enumeration of E. coli O157:H7 treated with ClO₂. The location and viability ofE. coli O157:H7 on uninjured and injured green pepper surfaces after ClO₂gas treatments were visualized using confocal laser scanning microscopy (CLSM). Live and dead cells of E. coli O157:H7 on pepper surfaces were labeled with a fluorescein isothiocyanate-labeled antibody and propidium iodide, respectively. A 7·27 log reduction of E. coli O157:H7 on uninjured green pepper surfaces was obtained with a 0·60 mg l⁻¹ClO₂gas treatment for 30 min at 20°C under 90–95% relative humidity. For injured surfaces, a 6·45 log reduction was achieved with a 1·2 mg l⁻¹ClO₂gas treatment. Each ClO₂gas treatment (0·15–1·2 mg l⁻¹ClO₂) for inoculated bacteria on uninjured surfaces showed significantly more reductions (1·23–4·24 log) than for those on injured surfaces (P<0·05). The microphotographs of CLSM showed that bacteria preferentially attached to injured surfaces and those bacteria could be protected from bacterial reduction by the injuries. This study indicates that ClO₂gas treatment can be a potential effective method of pathogen reduction for fresh fruits and vegetables.     

Iron‐mediated lipid oxidation in 70% fish oil‐in‐water emulsions: effect of emulsifier type and pH

The objective of this study was to investigate the protective effect of five different emulsifiers on iron‐mediated lipid oxidation in 70% fish oil‐in‐water emulsions. The emulsifiers were either based on protein (whey protein isolate and sodium caseinate) or based on phospholipid (soy lecithin and two milk phospholipids with different phospholipid contents, MPL20 and MPL75). Lipid oxidation was studied at pH 4.5 and 7.0, and results were compared to lipid oxidation in neat fish oil. Results showed that all emulsions oxidised more than neat oil. Furthermore, emulsions prepared with proteins oxidised more at low pH than at high pH, and casein emulsions oxidised the least (Peroxide value (PV) at day 7 was 0.5–0.7 meq kg^?1). Among emulsions prepared with phospholipids, emulsions with MPL75 were the most oxidised followed by emulsions prepared with lecithin and MPL20. Thus, PV in MPL75 emulsions was 5.0–5.5 meq kg^?1 at day 7 compared with 0.9–1.9 meq kg^?1 in MPL20 emulsions.     

Evaluation of <Emphasis Type=Italic>flaA</Emphasis> Short Variable Region Sequencing,Multilocus Sequence Typing,and Fourier Transform Infrared Spectroscopy for Discrimination between <Emphasis Type=Italic>Campylobacter jejuni</Emphasis> Strains

Discriminatory and robust typing methods are needed to improve the understanding of the dynamics of food-borne Campylobacter infections and epidemiology in primary animal production. To evaluate the strain discriminatory potential of typing methods, flaA short variable region (SVR) sequencing and Fourier transform infrared (FTIR) spectroscopy were applied on a collection of 102 epidemiologically related and unrelated Campylobacter jejuni strains. Previous application of FTIR spectroscopy for subtyping of Campylobacter has been limited. A subset of isolates, initially discriminated by flaA SVR sequencing, were further subjected to multilocus sequence typing (MLST). It was found that flaA SVR sequencing had a slightly higher discriminatory power than FTIR spectroscopy, based on the Simpson diversity index. The clustering of strains indicated that FTIR spectroscopy is indeed a suitable method for discrimination of Campylobacter. The isolates were assigned to six clusters based on flaA SVR sequences and nine clusters based on the FTIR spectroscopy profiles. Furthermore, the cluster analysis of flaA SVR sequences, MLST, and FTIR spectroscopy profiles showed a high degree of congruence, assigning the isolates to similar cluster structures. In conclusion, FTIR spectroscopy can be applied for subtyping of Campylobacter, and the high discriminatory potential of both flaA SVR sequencing and FTIR spectroscopy render them suitable screening methods for large numbers of strains.     

The choice of homogenisation equipment affects lipid oxidation in emulsions

Milk proteins are often used by the food industry because of their good emulsifying properties. In addition, they can also provide oxidative stability to foods. However, different milk proteins or protein components have been shown to differ in their antioxidative properties, and their localisation in emulsions has been shown to be affected by the emulsification conditions. The objective of this study was to investigate the influence of homogenisation equipment (microfluidizer vs. two-stage valve homogeniser) on lipid oxidation in 10% fish oil-in-water emulsions prepared with two different milk proteins. Emulsions were prepared at pH 7 with similar droplet sizes. Results showed that the oxidative stability of emulsions prepared with sodium caseinate was not influenced by the type of homogeniser used. In contrast, the type of homogenisation equipment significantly influenced lipid oxidation when whey protein was used as emulsifier, with the microfluidizer resulting in lower levels of oxidation.     

Mucoadhesive Electrospun Nanofiber-Based Hybrid System with Controlled and Unidirectional Release of Desmopressin

The sublingual mucosa is an attractive route for drug delivery, although challenged by a continuous flow of saliva that leads to a loss of drug by swallowing. It is of great benefit that drugs absorbed across the sublingual mucosa avoid exposure to the harsh environment of the gastro-intestinal lumen; this is especially beneficial for drugs of low physicochemical stability such as therapeutic peptides. In this study, a two-layered hybrid drug delivery system was developed for the sublingual delivery of the therapeutic peptide desmopressin. It consisted of peptide-loaded mucoadhesive electrospun chitosan/polyethylene oxide-based nanofibers (mean diameter of 183 ± 20 nm) and a saliva-repelling backing film to promote unidirectional release towards the mucosa. Desmopressin was released from the nanofiber-based hybrid system (approximately 80% of the loaded peptide was released within 45 min) in a unidirectional manner in vitro. Importantly, the nanofiber–film hybrid system protected the peptide from wash-out, as demonstrated in an ex vivo flow retention model with porcine sublingual mucosal tissue. Approximately 90% of the loaded desmopressin was retained at the surface of the ex vivo porcine sublingual mucosa after 15 min of exposure to flow rates representing salivary flow.     

Stochastic response of hysteretic systems

This paper deals with approximate stochastic response of hyseretic structures under white noise excitation, based on It stochastic differential equations. Instead of the original system an equivalent nonlinear system is considered, in which the drift vector is given by a series expansion of the order n 1 in terms of the state variables. n = 1 represents the well-known case of equivalent linearization. Components in the drift vector representing the nonanalytical constitutive equations are replaced by a cubic polynomial expansion. The hierarchy of statistical moment equations is closed by a cumulant neglect closure scheme. The method has been applied to a bilinear single degree-of-freedom system subjected to white noise excitation, for which an equivalent system with a cubic series expansion to the constitutive equation is considered. The results obtained are compared with those of numerical simulation and alternative methods, and they provide substantial improvements compared to equivalent linearization.     

A digital system for surface roughness analysis of plane and cylindrical parts

This paper describes an on-line system for digital analysis of surfaces where the electrical signal from a surface roughness instrument is converted and input into a desk-top computer which also controls the traversing. Special fixtures integrated with the system and dedicated software permit two- and three-dimensional tracings to be carried out on plane as well as on cylindrical parts. The surface profiles can be plotted in different ways. Roughness parameters, frequency spectra etc, can be computed digitally and all results can be stored. Scratches and irregularities on the surface can be identified and processed separately from the rest of the surface. The desk-top computer is connected to the university mainframe computer through a modem, this being of advantage in connection with graphic data processing. The capability of the equipment has been investigated and results from calibration are presented.     

Liquefaction,Saccharification and Isomerization of Starches from Sources Other than Maize

Examination of the suitability of a number of starches for the production of fructose syrup is described. In addition to maize starch, starch from wheat, potatoes and tapioca has been examined. Liquefaction, saccharification and purification were performed in pilot scale, whereas the produced syrups were isomerized in laboratory scale. The liquefactions were performed with Termamyl® in a jet cooker process. Saccharification with Amyloglucosidase Novo 150 L under standard conditions gave good DX values. After purification the syrups were isomerized in laboratory columns with Sweetzyme®. The results - activity and productivity of Sweetzyme - were for all syrups as good as or better than those obtained with dextrose solutions.     

Offshoring and backshoring of surface finishing from the perspective of the Nordic countries

Backshoring of production to Western Europe has become an increasingly important trend after decades of offshoring. The subject is introduced by a general discussion followed by a specific analysis of the Nordic surface finishing industry. The main finding is that production quality is the main driver for backshoring of surface finishing.