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21.
H. Wahl K. Herzog C. Federspiel P. Juza 《钢铁》2004,39(Z1):593-600
1前言
生产率和作业率方面的收益以及可能性调整的灵活性是提高钢铁工业竞争力的关键因素. 相似文献
22.
The electrochemical copolymerization of furan and thiophene was performed at a constant electrode potential in a binary solvent system consisting of boron trifluoride/ethyl ether and an additional amount of ethyl ether (molar ratio = 1 : 2). The obtained homopolymers and copolymers were characterized with cyclic voltammetry and infrared spectroscopy. The influence of the applied electropolymerization potential and the monomer feed ratio of furan and thiophene on the copolymers was investigated. The furan–thiophene copolymers showed good stability of the redox activity in an acetonitrile‐based electrolyte solution. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2008 相似文献
23.
Rudolf Felix 《Soft Computing - A Fusion of Foundations, Methodologies and Applications》2008,12(2):129-135
Real world applications of a decision model of relationships between goals based on fuzzy relations are presented. In contrast
to other approaches the relationships between decision goals or criteria for each decision situation are represented and calculated
explicitly. The application fields are decision making for financial services and optimization of production sequences in
car manufacturing. 相似文献
24.
Rudolf Kunz 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1906,12(11):641-645
Ohne ZusammenfassungMitteilung aus der K.-k. allgemeinen Untersuchungsanstalt für Lebensmittel in Wien. 相似文献
25.
Y Sheng A Sali H Herzog J Lahnstein SA Krilis 《Canadian Metallurgical Quarterly》1996,157(8):3744-3751
beta2-Glycoprotein I (beta2GPI) is a phospholipid-binding serum protein with anticoagulant properties. It plays a vital role in the binding of anti-cardiolipin Abs purified from patients with autoimmune disease when assayed in a cardiolipin (CL) ELISA. Based on a three-dimensional model of beta2GPI, electrostatic calculations, and earlier peptide studies, a highly positively charged amino acid sequence, Lys282-Asn-Lys-Glu-Lys-Lys287, located in the fifth domain of beta2GPI, has been predicted to be the phospholipid binding site. We tested this hypothesis by site-directed mutagenesis of residues in the predicted phospholipid binding site and by assessing the mutants for phospholipid binding and anti-beta2GPI activity. A single amino acid change from Lys286 to Glu significantly decreased the binding of beta2GPI to CL. Double and triple mutants 2k (from Lys286, 287 to Glu286, 287), 2ka (from Lys284, 287 to Glu284, 287), and 3k (from Lys284, 286, 287 to Glu284, 286, 287) possessed no binding of Ab to beta2GPI in a CL ELISA, as well as no inhibitory activity on the binding of iodinated native beta2GPI to CL. These results indicate that the residues Lys284, Lys286, and Lys287 in the fifth domain of beta2GPI are critical for its binding to anionic phospholipids and its subsequent capture for binding of anti-beta2GPI Abs. 相似文献
26.
Michael Kofink Menelaos Papagiannopoulos Rudolf Galensa 《European Food Research and Technology》2007,225(3-4):569-577
The enantiomers of catechin and epicatechin were separated by chiral capillary electrophoresis using modified cyclodextrins
as chiral selectors. Various conditions for the separation system were optimized, including the pH value and the concentration
of the running buffer. A baseline separation of the catechin and epicatechin enantiomers could be achieved by using 0.1 mol l−1 borate buffer (pH 8.5) with 12 mmol l−1 (2-hydroxypropyl)-γ-cyclodextrin as chiral selector, a fused-silica capillary with 40 cm effective length (75 μm I.D.), +18 kV
applied voltage, a temperature of 20 °C and direct UV detection at 280 nm. The method was applied to different plant food
samples. (+)-Catechin and (−)-epicatechin could be verified as the most common flavan-3-ols. In the case of guaraná, however,
we were able to identify all four enantiomers, both (+)- and (−)-catechin and (+)- and (−)-epicatechin, as naturally occurring
compounds. This finding was verified by further isolation and purification of the flavan-3-ols and subsequent LC–MS analysis.
This method allows for the identification of the authenticity of guaraná through the analysis of the catechin and epicatechin
enantiomers, additionally to the conventional methods like HPLC. 相似文献
27.
Rudolf E. Schmitt Johann Haas und Renato Amadò 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1988,187(2):121-124
Zusammenfassung Zur Bestimmung biogener Amine aus Schlachtgeflügel wird ein einfach durchzuführendes Analysenverfahren beschrieben. Die Amine wurden mit 0,6m-Perchlorsäure extrahiert, nach Dansylierung auf einer RP-18 Säule aufgetrennt und bei 254 nm detektiert. Innerhalb von 7 min konnte eine gute Auftrennung von 8 Aminen erreicht werden. Zur quantitativen Erfassung von Spermin und Spermidin mußte der Extrakt zusätzlich über Amberlite CG 50 gereinigt werden. Die Wiederfindungsraten betrugen 82% bis 96%, die Nachweisgrenzen 0,2 bis 0,5 g pro g Hühnerhaut. Bei Schlachtgeflügel eignen sich Putrescin und Cadaverin gut als Leitsubstanzen für den einsetzenden Verderb, da sie bereits ab Gesamtkoloniezahlen von etwa 105 pro cm2 nachweisbar sind, und ihre Konzentration mit zunehmendem Verderb rasch ansteigen.
Determination of biogenic amines by RP-HPLC for monitoring microbial spoilage of poultry
Summary A simple high-performance liquid chromatographic (HPLC) analysis is described for the determination of biogenic amines in broiler carcasses. The clean-up procedure consists of an extraction with 0.6M-perchloric acid, formation of dansyl derivatives, separation by a RP-18 column and UV detection at 254 nm. Within 7 min eight amines could be separated. The quantitative determination of spermidine and spermine requires an additional ion-exchange clean-up with Amberlite CG 50 after the extraction. This procedure gives recoveries of 82%–96% with detection limits of 0.2–0.5 g/g of broiler skin. Putrescine and cadaverine are good indicators for the onset of spoilage of poultry carcasses, since both amines could be detected at total colony counts of 105 cfu/cm2 and their concentration increases rapidly with advancing decomposition.相似文献
28.
Nadine Kretschmer Antje Hufner Christin Durchschein Katrin Popodi Beate Rinner Birgit Lohberger Rudolf Bauer 《International journal of molecular sciences》2021,22(5)
Melanoma is the deadliest form of skin cancer and accounts for about three quarters of all skin cancer deaths. Especially at an advanced stage, its treatment is challenging, and survival rates are very low. In previous studies, we showed that the constituents of the roots of Onosma paniculata as well as a synthetic derivative of the most active constituent showed promising results in metastatic melanoma cell lines. In the current study, we address the question whether we can generate further derivatives with optimized activity by synthesis. Therefore, we prepared 31, mainly novel shikonin derivatives and screened them in different melanoma cell lines (WM9, WM164, and MUG-Mel2 cells) using the XTT viability assay. We identified (R)-1-(1,4-dihydro-5,8-dihydroxy-1,4-dioxonaphthalen-2-yl)-4-methylpent-3-enyl 2-cyclopropyl-2-oxoacetate as a novel derivative with even higher activity. Furthermore, pharmacological investigations including the ApoToxGloTM Triplex assay, LDH assay, and cell cycle measurements revealed that this compound induced apoptosis and reduced cells in the G1 phase accompanied by an increase of cells in the G2/M phase. Moreover, it showed hardly any effects on the cell membrane integrity. However, it also exhibited cytotoxicity against non-tumorigenic cells. Nevertheless, in summary, we could show that shikonin derivatives might be promising drug leads in the treatment of melanoma. 相似文献
29.
Rudolf Rothen 《特种铸造及有色合金》2005,(Z1):140
全新的工艺数据永久记录,对压铸过程进行最优化控制满足适用并易于使用的市场需求.布勒公司新的dat@net控制系统(见图1和图2),综合了传统技术,实时操作,工业耐用性和稳定性,以及当今最先进的PCj技术的优点.精心的硬、软件匹配允许使自动化方案不受约束.也是首次,这种控制系统使压铸设备能够按照客户需求完全协调. 相似文献
30.
Summary The effect of tert-butyl chloride in the polymerizations of isobutylene carried out in the presence of SnCl4 in dichloromethane at temperatures-20°C and-78°C was investigated. Synthesized polyisobutylene samples showed a bimodal molecular
weight distribution (MWD) and it was found that the weight content of the lowermolecular weight (LMW) fraction increased with
increasing t-BuCl concentration in the polymerization mixture. The effect of ageing of the initiation mixture t-BuCl/SnCl4 in CH2Cl2 prepared in advance on MWD of the PIB samples was also studied. Ageing of the initiation system supports the formation of
the LMW fraction and this oily PIB is the only product of the polymerization at-20°C,
. 相似文献