Lactobacillus acidophilus: Characterization of the Species and Application in Food Production

L. acidophilus is a homofermentative, microaerophilic, short chain gram positive microorganism with rod morphology having its bacteriocins belonging to class II a. Several bacteriocins of L. acidophilus have been isolated and characterized. These are structurally similar, but their molecular weight varies as well as their spectrum of antimicrobial activity. They exhibit important technical properties, i.e., thermostability and retaining of activity at a wide pH range along with strong inhibitory actions against food spoilage and pathogenic bacteria make them an important class of biopreservatives. L. acidophilus can be added as an adjunct in many food fermentation processes contributing to unique taste, flavor, and texture. It also preserves the products by producing lactic acid and bacteriocins. A lot of new information regarding the bacteriocins of L. acidophilus has emerged during the last few years. In this review, an attempt has been made to summarize and discuss all the available information regarding the sources of bacteriocins production, their characteristics, and their antimicrobial action along with their application.     

Laser welding of Haynes 188 alloy sheet: thermal stress analysis

Laser welding of Haynes 188 alloy sheets is carried out. Temperature and stress fields developed in the welding section are predicted numerically incorporating the finite element algorithm. The microstructural changes in the welding region are examined using the optical and electron scanning microscopes. The microhardness variation across the weld zone is measured and associated with the residual stress formed in this region. It is found that the microhardness follows the residual stress distribution in the weld core. The maximum residual stress is less than the elastic limit of the substrate material.     

A flavoprotein monooxygenase that catalyses a Baeyer-Villiger reaction and thioether oxidation using NADH as the nicotinamide cofactor

A gene from the marine bacterium Stenotrophomonas maltophilia encodes a 38.6 kDa FAD‐containing flavoprotein (Uniprot B2FLR2) named S. maltophilia flavin‐containing monooxygenase (SMFMO), which catalyses the oxidation of thioethers and also the regioselective Baeyer–Villiger oxidation of the model substrate bicyclo[3.2.0]hept‐2‐en‐6‐one. The enzyme was unusual in its ability to employ either NADH or NADPH as nicotinamide cofactor. The K_M and k_cat values for NADH were 23.7±9.1 μM and 0.029 s^?1 and 27.3±5.3 μM and 0.022 s^?1 for NADPH. However, k_cat/K_M value for the ketone substrate in the presence of 100 μM cofactor was 17 times greater for NADH than for NADPH. SMFMO catalysed the quantitative conversion of 5 mM ketone in the presence of substoichiometric concentrations of NADH with the formate dehydrogenase cofactor recycling system, to give the 2‐oxa and 3‐oxa lactone products of Baeyer–Villiger reaction in a ratio of 5:1, albeit with poor enantioselectivity. The conversion with NADPH was 15 %. SMFMO also catalysed the NADH‐dependent transformation of prochiral aromatic thioethers, giving in the best case, 80 % ee for the transformation of p‐chlorophenyl methyl sulfide to its R enantiomer. The structure of SMFMO reveals that the relaxation in cofactor specificity appears to be accomplished by the substitution of an arginine residue, responsible for recognition of the 2′‐phosphate on the NADPH ribose in related NADPH‐dependent FMOs, with a glutamine residue in SMFMO. SMFMO is thus representative of a separate class of single‐component, flavoprotein monooxygenases that catalyse NADH‐dependent oxidations from which possible sequences and strategies for developing NADH‐dependent biocatalysts for asymmetric oxygenation reactions might be identified.     

High Coercivity and Magnetization in WSe2 by Codoping Co and Nb

Introducing ferromagnetism in transition metal dichalcogenides has attracted lots of attention due to the possible applications in spintronics devices. Generally, single magnetic element doping is used to introduce magnetism. However, mostly, weak ferromagnetism is observed. In this work, codoping of two kinds of transition metals (Nb and Co) into WSe₂ is used to study its magnetic properties. In detail, single crystal WSe₂ is codoped with 4 at% Co and various concentrations of Nb by employing the physical ion implantation method. Raman, X‐ray diffraction and X‐ray photoelectron spectroscopy results reveal the effective substitutional doping of implanted elements (Co and Nb). Magnetic measurements illustrate that both un‐doped and 4 at% Co doped WSe₂ show weak ferromagnetism whereas magnetization is strongly enhanced when Co and Nb are codoped into WSe₂. The magnetization is comparable with a ferromagnet, which may be attributed to Co, Nb doping and defects. In addition, a large coercivity of ≈1.2 kOe is observed in the 1 at% Nb–4 at% Co codoped WSe₂ sample, which may be ascribed to the combined effect of doping‐induced stress, defect‐dictated pinning and anisotropy of Nb? Se bond owing to the charge transfer between Nb and Se ions.     

Intelligent noise detection and filtering using neuro-fuzzy system

In this paper, we propose a neuro-fuzzy based blind image restoration to remove impulse noise from low as well as highly corrupted images. Main components of the proposed technique include noise detection, histogram estimation and noise filtering process. Proposed technique constructs the fuzzy sets using fuzzy number construction algorithm. These fuzzy sets are used in noise filtering process to remove impulse noise from the noisy pixels using neuro-fuzzy inference system and fuzzy decider. Experimental results are based on global and local error measures, which prove that the proposed technique gives superior results than the present well known impulse noise filtering methods.     

HIGH PRESSURE DENSITIES OF MIXTURES OF COAL CHEMICALS

Densities of seven coal chemicals (benzene, toluene, letralin, quinoline, m-cresol, bicyctohexyl and 1-methylnapthalene) and their binary mixtures at temperatures between 298 K and 338 K and pressures between 0.1 MPa and 34.5 MPa were measured using a high-pressure vibrating tube densitometer and are reported in this paper. The pure component densities were correlated using a modified Tail equation. The pure components were then used as reference fluids in a corresponding stales method for the prediction of the densities of the mixtures. @KEYWORDS High pressure Densities Mixtures Coal chemicals.     

One-step hydroxylation of benzene to phenol. II. Gas phase N2O oxidation over Mo/Fe/borosilicate molecular sieve

The Fe/Mo/partially deboronated borosilicate molecular sieve catalyst prepared by the chemical vapor deposition (CVD) method was active for the selective formation of phenol by gas-phase N₂O oxidation of benzene. The impregnated counterpart exhibited lower activity than the CVD catalyst. The borosilicate molecular sieve itself also was active. Two mechanistic paths are postulated based on reactive oxygen species such as O^–, which can be generated via interaction of N₂O with the iron sites in the CVD-borosilicate molecular sieve catalyst, or OH⁺, which can be generated by Brønsted sites on the borosilicate molecular sieve itself.     

Design, implementation and testing of electrostatic SOI MUMPs based microgripper

This paper presents a detail modeling, finite element analysis and testing results of MEMS based electrostatically actuated microgripper. Interdigitated lateral comb pairs have been used to actuate the microgripper. The microgripper is optimized using standard SOI-MUMPs technology in L-Edit of MEMS-Pro with dual jaws actuation at low voltages. Coupled electromechanical finite element analysis performed in COVENTOR-WARE shows total displacement of 15.5 μm at jaws tip at 50 V, which is quite comparable to experimental result of 17 μm displacement at the tip of gripper jaw for the same voltage. Micromanipulation experiments have successfully demonstrated the gripping, holding micro-objects between 53 and 70 μm in size. The simulated model is used to study detail profile of Von Mises stresses and deformations in the model. It is noted that maximum stress in microgripper is 200 MPa which is much smaller than yield stress of 7 GPa. The slight difference between finite element analysis and experimental results is because of small variations in process material parameters. The total size of gripper is 5.03 × 6.5 mm².     

Comparison of treatment records and inventory of empty drug containers to quantify antimicrobial usage in dairy herds

Assessment of antimicrobial use (AMU) is vital for interpreting the origin of changes in antimicrobial resistance (AMR). The objectives of the present study were to estimate the association between AMU determined using on-farm treatment records (TR) and inventory of empty drug containers (INV). Herds were selected to represent Canadian dairy farms. Producers were asked to record animal health events and treatments on a standard General Health Event form. For inventory data, 40-L receptacles were placed at various locations considered convenient to deposit all empty drug containers. Antimicrobial defined-daily dosages (ADD) were calculated for 51 Canadian herds using the 2 methods. Estimation of AMU was 31,840 ADD using the INV and 14,487 ADD using the TR, indicating that for every TR entry, 2.20 times more treatments were observed using the INV. Mastitis, reproductive conditions, and dry cow therapy were the most frequent reasons for antimicrobial therapy when assessing TR. For all antimicrobials evaluated, mean ADD was higher using the INV versus TR. Regardless, a strong positive correlation (0.80) was observed between the 2 methods, indicating that herds with increased number of ADD recorded using the INV also had increased number of ADD recorded using TR. Furthermore, a positive association was observed for the 6 most commonly used antimicrobials. In comparison to methods used in surveillance programs on AMU in livestock that assume a constant use in all herds (i.e., sales data), INV provided a herd-level specific quantity of AMU positively correlated with AMU recorded at the animal level in general. The INV was easy to implement and provided a measure of total AMU in the herd. Availability of such information would be valuable for interpreting changes in AMR at the herd level and enabling evaluation of interventions for decreasing AMR.