Inhibition of diabetic nephropathy by a GH antagonist: a molecular analysis

Streptozotocin-treated C57B1/SJL mice developed glomerular hypertrophy and light microscopic lesions mimicking human diabetic glomerulosclerosis. In contrast, there were no glomerular hypertrophy and lesions in diabetic mice transgenic (TG) for a mutated growth hormone (bGH-G119K) that competes with native endogenous GH and results in dwarfism. We examined the molecular events underlying these findings. The non-transgenic (non-TG) diabetic mouse glomeruli had an increase in mRNA coding for alpha 1IV collagen, laminin B1, TGF-beta 1, 72 kDa collagenase, and TIMP-3. In contrast, glomerular type IV collagen and laminin B1 mRNA levels were normal in diabetic TG dwarf mice. However, the 72 kDa gelatinase, TIMP-3, and TGF-beta 1 mRNAs were elevated in the diabetic dwarfs. Type IV collagen and laminin accumulated in the glomeruli of diabetic non-TG, but not of diabetic dwarf mice, by immunofluorescence microscopy, confirming the mRNA data. GH binding protein mRNA levels were comparable in glomeruli from dwarf and non-TG mice, both diabetic and non-diabetic. We did not detect GH receptor mRNA in glomeruli. These data suggest that diabetic glomerulosclerosis is associated with an increase in type IV collagen and laminin synthesis, and that these changes do not occur in mice transgenic for bGH119K, a functional antagonist of GH. The increase of 72 kDa gelatinase, TIMP-3 and TGF-beta 1 mRNAs, independent of GH, suggested that these changes induced by hyperglycemia were not sufficient for the induction of glomerulosclerosis.     

Differential suppression of dialysis patients' lymphocyte IFN-gamma production by glucocorticoids and cyclosporine

IFN-gamma is a potent pro-inflammatory cytokine involved in the immunologic rejection of transplanted organs. Having previously demonstrated differential suppressive effects of methylprednisolone (MP), prednisolone (P) and cyclosporine (CsA) on dialysis patients' lymphocyte proliferative responses to phytohaemagglutinin (PHA), we studied the effects of these drugs on dialysis patients' lymphocyte IFN-gamma production during mitogenic and allogeneic (MLR) stimulation. The mean +/- SEM 50% inhibitory concentration (ng/ml) on cell proliferation was significantly lower for MP than P in PHA-stimulated haemodialysis (HD) patients' (35 +/- 7 vs 152 +/- 25, P < 0.001) and peritoneal dialysis (PD) patients' (35 +/- 11 vs 134 +/- 33, P = 0.001) cultures and in HD patients' MLR cultures (15 +/- 3 vs 48 +/- 9, P < 0.001). The mean +/- SEM fractional responses (PHA or MLR + drug/PHA or MLR) in culture supernatant IFN-gamma concentrations were significantly lower with 10(-7) M concentrations of MP than P in HD (0.19 +/- 0.05 vs 0.31 +/- 0.06, P = 0.01) and PD (0.30 +/- 0.11 vs 0.46 +/- 0.11, P < 0.05) PHA cultures and in HD MLR cultures (0.15 +/- 0.04 vs 0.28 +/- 0.07, P = 0.01). CsA (400 ng/ml) alone not only caused less than 50% inhibition of IFN-gamma production in 15/27 HD PHA, 6/14 PD PHA and 4/13 HD MLR cultures, but actually stimulated it in 9 HD and 5 PD PHA cultures. The results suggest that: (1) MP has greater immunosuppressive potential than P for renal transplant recipients; (2) the stimulation of IFN-gamma by CsA in some patients could be harmful in patients with initial allograft dysfunction; and (3) pre-transplant in-vitro assessment of recipients' PBMC responsiveness to glucocorticoids and CsA may help individualize the post-transplant immunosuppressive regimen.     

非定常Stokes问题解的存在唯一性的正则性

引入Ｆａｄｅｏ－Ｇａｌｅｒｋｉｎ方法解决了三维非定常半周期Ｓｔｏｋｅｓ问题解的存在唯一性问题,在加强了初始条件后,得到了解的正则性结果。     

基于BP网络模型的立项评估研究与应用

在项目管理立项评估数据的基础上,通过科学设计BP神经网络模型结构,结合Matlab7.0软件将BP算法的评估模型首次应用在科研项目的立项评估中。通过科研项目立项评估模型的实际运行,证明了该评估算法的有效性,有力提高了科研部门的立项评估水平。     

分布式装备保障信息应用集成框架研究与应用

为适应信息化战争的需要,信息系统被应用到各类装备保障领域以提高信息化水平.但是从装备保障的宏观角度来看,装备保障信息被封闭在相互独立而且分布的系统中,造成一个个信息孤岛,这就要求对装备保障信息进行应用集成.针对传统集成框架在实际应用集成中的不足,分析了对.NET平台以及Web服务在分布式异构环境中应用集成实现的可行性,构建了一种具有自身特点的分布式装备保障信息应用集成框架.该框架可以充分利用现有信息资源,以较少的代价摆脱信息化建设中信息孤岛和数据利用率不高的困境,解决方案具有较为普遍的适用性.     

改进行移编码的文本数字水印算法研究

研究了文本数字水印在追溯涉密纸质文档中的重要意义,介绍了常见的文本数字水印技术,分析了Brassil经典行移编码文本数字水印技术,在算法的标记策略中引入检验编码,文本行分割划段和引入基调行三个方面进行改进,改进后算法具有更大水印信息容量,更强的鲁棒性和可控的计算量.     

微扑翼飞行中的生物和仿生力传感器述评

随着昆虫飞行机理和仿生扑翼飞行研究的不断进展,理解昆虫的传感系统并研制适于微型扑翼飞行器的仿生传感器逐渐成为目前关注的焦点,而MEMS的快速发展使得研制新型的仿生微型传感器成为可能。简要介绍仿生扑翼飞行中传感系统研究现状,对昆虫飞行中采用的力传感器原理、分类进行了介绍;重点分析了几种典型的力传感器;归纳和总结了基于昆虫毛状传感器、钟状传感器和平衡棒仿生,采用各种MEMS技术研制的微型力传感器的生产工艺和应用特点;探索和展望了仿生力传感器的发展特点和今后的发展趋势。     

C／T iO 2复合材料的制备及光催化性能的研究

采用溶胶-凝胶法制得了C/TiO2复合光催化剂,并采用X射线衍射（XRD）﹑扫描电子显微镜（ SEM ）﹑紫外光谱等方法对复合材料进行了表征。以1-萘胺-4-偶氮对苯磺酸为模型化合物评价了C/TiO2复合材料的光催化性能,考察了降解时间、初始浓度和催化剂投加量等因素对降解率的影响。研究表明,在1-萘胺-4-偶氮对苯磺酸初始浓度为2×10-5 mol/L,催化剂投加量为1．0 g/L时,光催化反应5 h后,1-萘胺-4-偶氮对苯磺酸的降解率达到85．41％。     

对建立中国特色养老居住形式的思考

针对新世纪我国人口老龄化问题，从比较中外养老体制差异和分析我国养老特点入手，着重介绍家庭单元式养老住宅，希望利用合理的养老住宅设计加强对家庭养老这种具有中国特色养老居住形式的促进作用，为老人提供一个更加健康舒适的养老环境。