一种5 重real-time PCR筛查转基因水稻方法的建立

以转基因水稻中最常用的CaMV35S启动子、NOS终止子、Cry1Ab/Ac基因、HPT基因及SPS水稻内标基因为研究对象，利用5 种不同的荧光信号（FAM、HEX、Taxas Red、Cy5、Cy5.5）进行多重实时聚合酶链式反应（real-time polymerase chain reaction，real-time PCR）检测方法的研究。通过引物组合筛选、反应体系优化、特异性测试、灵敏度测试、适用性测试等一系列实验，建立了5 重real-time PCR方法，灵敏度可达0.032%。此方法具有灵敏度高、结果准确、通量大等优点，可实现水稻中转基因成分的快速、高效检测。     

液相色谱法快速检测染色梅鱼中日落黄、柠檬黄的含量

目的建立一种快速、低成本检测染色梅鱼中日落黄、柠檬黄的方法。方法将染色梅鱼剥皮,鱼皮经70%甲醇氨水提取液提取,经EclipseXDB-C_(18)色谱柱分离,以甲醇和乙酸铵水溶液为流动相,进行梯度洗脱,流速为1.0 mL/min,柱温35℃,利用紫外检测器检测日落黄、柠檬黄的含量。结果日落黄、柠檬黄在1.25、2.5、12.5、25 mg/kg添加水平的加标回收率为80%～90%,相对标准偏差小于10%(n=6),柠檬黄检出限为0.05 mg/kg,日落黄检出限为0.025 mg/kg。结论该方法快速、准确、成本低,适合染色梅鱼中日落黄、柠檬黄的快速检测。     

宝山铅锌银矿石选矿现状与对策

对宝山铅锌银矿石原矿性质、工艺流程、药剂制度及技术指标情况进行了介绍,指出了存在粉矿粒度过粗、磨矿效果不佳、金银回收率过低、矿种变化频繁及原矿品位波动大等问题,同时从降低粉矿粒度、完善磨矿自动化、浮选采用X荧光在线分析仪,有效回收金银矿物、合理配矿等方面,提出了解决问题的对策。     

节能技术在现代城市路灯照明中的应用

介绍节能技术在现代城市路灯照明中的应用.     

基于综合用电煤耗的电力高质量发展评价指标研究及应用

煤电平均供电煤耗作为评价能源系统效率的指标已在行业内广泛应用。但该项指标无法很好地反映一个国家、地区的用电结构与用电能耗水平。为此，参照行业内普遍采用的火电厂发电煤耗和供电煤耗指标，定义“综合用电煤耗”，即用户每用1 kW?h电“消耗”的标准煤量（单位:g/(kW?h)）。在此基础上，提出“综合发电煤耗”与“综合供电煤耗”的概念，形成完整的指标评价体系。对近年典型省份指标值的研究表明，该指标体系能够很好地反映供给侧国家、省区或企业的发电结构与电能生产能耗水平。这一指标体系能够为正确理解和计算用电、输电与电能替代项目的化石能源消耗量及其排放量提供依据。     

新型计量斗在锰粉料制块配料中的改进应用

锰粉料制块是解决铁合金冶炼精料入炉的主要途径之一,生产过程中为解决锰粉料粘附计量斗的问题,介绍了一种新型计量斗,阐述他的结构特点和工作原理,实施改进后下料流畅,降低了劳动强度,提高了生产效率。     

《医学化学》课程教学质量提升策略研究

根据高校新时期教学发展理念,从优化课程内容体系、改革课程评价方式、强化各种教学手段、培养学生的实验创新能力、充分应用各种教学资源等方面改革《医学化学》课程。使其成为贴近学生、贴近课堂实际需求的一门高质量课程。提高生物信息学、生物医学工程等专业学生的学习能力及综合素质。为生物、医学、信息等相关专业构建起扎实、系统的基础知识体系,从而达到高校创新教育的目标。     

Smart firefighting construction in China: Status,problems, and reflections

“Smart firefighting” construction as a part of the “smart city” has been a concern of the public security and fire agencies at all levels. In this study, the status, problems, and reflections of “smart firefighting” construction in China are discussed. A recent survey indicates that China has launched its smart firefighting construction and initially created a new perspective on its regional smart firefighting work based on three main aspects: intelligent disaster perception by Internet of Things (IoT) construction, intelligent disaster prevention by big data construction, and intelligent disaster disposal by emergency rescue platform construction. However, the current smart firefighting construction in China still has some prominent problems such as the data interconnectivity and normalized management of various platforms, the extensibility of smart firefighting platforms, and the intelligent level of smart firefighting researches, which need to be solved urgently. Therefore, we argue that smart firefighting construction in China should establish data interconnectivity, industrial normalized management, 2D/3D geographic information interaction and extension, high-integration fire protection theory, and many other aspects in the near future and truly realize firefighting visualization and efficient data applications for 4D time space. This study could provide valuable reference for smart firefighting and smart city construction.     

Research on the identification performance of UHF RFID system considering the correlation of cascaded channel

The identification rate of UHF RFID system was restricted by multipath propagation effects.The system identification performance was studied considering the correlation coefficient between forward and reverse channels.Based on the generalized Rician fading channel model,the analytical expression of identification rate was derived under independent,full correlation and correlation cases.Compared with the existing analysis,the proposed uniform calculation formula of identification rate was for any correlation coefficient and kinds of channel conditions.The numerical computation and Monte-carlo simulations show that the influences of different correlation coefficients,channel conditions,sensitivity and distance on the identification rate.     

智能化救援监控系统在煤矿安全生产中的应用

针对煤矿发生事故后传统救援监控系统无法实时对井下人员进行动态定位，导致矿井救援盲目性大、救援效率差、救援难度大等技术难题，为了进一步提高煤矿救援效率，通过技术研究，设计了一套以通信基站为核心的智能化救援监控系统，分析了该系统结构组成、工作原理，通过在担水沟煤矿井下实际应用效果来看，智能化救援监控系统对人员定位精准度达95%，实现人员动态位置三维成像，救援效率提高至80%以上，有效缩短了煤矿事故救援时间，取得了显著应用成效。