Thermodynamic stability of immunoglobulins and allosteric interactions with ferritin and protein A: distinct properties of the two antibodies of IgG2a subclass

The two anti-ferritin monoclonal antibodies of mouse IgG2a subclass, G10 and F11, are described that have similar affinity to human spleen ferritin and identical protein A-binding affinity. Antigen binding was shown to change significantly the protein A-binding parameters of the IgG2a antibodies. Antigen-induced conformational changes result in enhanced protein A-binding affinity of the G10 antibody while reduced affinity of the F11 antibody. Antigen binding does not change inherently low affinity of the anti-ferritin IgG1 antibody C5 to protein A. Differential scanning calorimetry revealed that the enthalpy and Gibbs free energy of denaturation for G10 was respectively by 19 and 29% higher than the corresponding parameters for F11. The lower structural energetics of F11 is associated with the lack of a calorimetrically revealed folding unit that may be responsible for distinct interaction between the antigen-binding and protein A-binding sites. This work provides experimental demonstration of the fact that functionally significant interactions between the two spatially remote recognition sites in antibodies of the same heavy chain isotype can be modulated by relatively small structural variations that also result in different thermodynamic stability.     

Cyclohexane transformation during water chlorination

Cyclohexane transformation was studied during water chlorination. Three mechanisms of cyclohexane transformation in the presence of chlorine were determined. Over 70 reaction products were identified. Five of them have mutagenic activity.     

钒对高铬白口铸铁凝固和显微组织的影响

论述了Fe-C-Cr-V合金的凝固特点,以及钒对该合金系显微组织的影响.当w（V）＜5%时,钒使合金的共晶点稍向右移;当w（V）＞5%时,钒使共晶点左移.w（V）量大于2%后,钒能抑制w（Cr）/w（C）＜5的Fe-C-Cr合金M3C的形成,得到M7C3型碳化物,含钒的Fe-C-Cr-V合金,由于VFe结晶后不久发生（γFe＋MC）共晶反应,缩短了γFe枝晶长大的温度范围,使γFe枝晶细化.指出了为得到MC体积量＞M7C3体积量的C、Cr、V含量适宜范围.     

展望高铬铸铁的发展

简述高铬白口铸铁发展历程.在降低铸铁中气体类夹杂物的含量和细化碳化物的基础上,未来高铬白口铸铁的显微组织中,碳化物体积百分数将可高达60%,其抗磨性能将会大幅度提高.     

供电局管理强本创新,为企业发展提供能源保障

介绍了大理供电局在落实南网“完善、规范、巩固、提高”的总体要求及“六个更加注意”的工作方针,加强管理,强本、创新,为企业经济发展提供了能源保障。     

Assessment of homeostasis by body''s adaptative responses in patients with tuberculous pleurisy]

AIM: The study of the detection rate and potential pathogenetic significance of antibodies to low density oxidated lipoprotein (LDOL) in patients who had ischemic cerebral circulation disorder (ICCD) at young age. MATERIALS AND METHODS: The examination (enzyme immunoassay, general and neurological investigations, laboratory and instrumental tests) covered 148 patients who survived ICCD at young age (mean age 37.2 years). RESULTS: 48 of 108 (44%) patients had LDOL antibodies. Antibodies to cardiolipin, lupus anticoagulant were recorded in 33 (31%) patients. LDOL antibodies were higher and occurred more frequently in patients with Sneddon's syndrome (35% of patients, mean LDOL antibodies-44 units) and primary antiphospholipid syndrome (17% of patients, 45 units) than in atherosclerotic affection of the major head arteries (4%, 29 units) or occlusion of cerebral arteries of unclear genesis (8% of patients, 29 units). CONCLUSION: ICCD were not related to fast development of cerebral atherosclerosis or periaortitis due to production of LDOL antibodies as no relationships were found between their rise and atherosclerotic lesions of cerebral major arteries or periaortitis as shown by ultrasonic dopplerography or cerebral angiography. Feasibility of LDOL antibodies participation in the coagulation cascade and induction of hypercoagulatory condition causing ICCD needs special investigation.     

基于侧抑制网络的红外图像预处理

侧抑制网络具有"增强反差,突出边框"的功能,但红外图像经其处理后,丢失了图像的部分细节.提出将其与原图像加权叠加在一起,该方法不仅突出了图像边缘,而且克服了上述不足.通过仿真验证了方法的有效性.