Development and study of anodic Ti/TiO2 electrodes and their potential use as impedimetric immunosensors

Titanium dioxide films were anodically formed at various potentials up to 65 V in 1 M H₂SO₄. Oxide films were characterized by performing various techniques, including electrochemical impedance spectroscopy, scanning electron microscopy, Raman spectroscopy, ellipsometry and diffuse reflectance FT-IR spectroscopy. Low voltage anodization (up to 10 V) results to amorphous TiO₂, whereas at higher applied potentials (up to 65 V), anatase is the predominant form. Anatase films were further hydroxylated with an acidic agent and the effect of this treatment on the overall impedance of the electrodes was studied with impedance spectroscopy. The potential use of anodic (anatase) Ti/TiO₂ electrodes in the development of impedimetric immunosensors is also demonstrated by monitoring the immunoreaction of avidin/anti-avidin with different instrumental approaches based on a FRA analyzer, an LCR-meter and a home-built charge integrator (Multipulser).     

Shelf-life extension of refrigerated Mediterranean mullet (Mullus surmuletus) using modified atmosphere packaging

The present work evaluated the quality and freshness characteristics and the effect of modified atmosphere packaging (MAP) on the shelf-life extension of refrigerated Mediterranean mullet using microbiological, biochemical, and sensory analyses. Fresh open sea red mullet (Mullus surmuletus) were packaged in four different atmospheres: M1, 10%/20%/70% (O2/ CO2/N2); M2, 10%/40%/50% (O2/CO2/N2); M3, 10%/60%/30% (O2/CO2/N2); identical fish samples were packaged in air. All fish were kept under refrigeration (4 +/- 0.5 degrees C) for 14 days. Of the three gas atmospheres, the 10%/40%/50% (M2) and 10%/ 60%/30% (M3) gas mixtures were the most effective for inhibiting growth of aerobic microflora in mullet samples until day 10 of refrigerated storage. H2S-producing bacteria and pseudomonads were part of the mullet microflora and their growth was partly inhibited under MAP conditions. Between these two bacterial groups, H2S-producing bacteria (including Shewanella putrefaciens) were dominant toward the end of the storage period, regardless of the packaging conditions. Brochothrix thermosphacta and lactic acid bacteria were found to be members of the final microbial flora of MAP and air-packaged mullet, whereas the Enterobacteriaceae population was lower than other bacterial groups. Of the chemical freshness indices determined, thiobarbituric acid values were variable in mullet samples irrespective of packaging conditions indicative of no specific oxidative rancidity trend. Based on sensorial data and aerobic plate count, trimethylamine nitrogen and total volatile basic nitrogen limit values in the range of ca. 15 to 23 and 52 to 60 mg N/100 g of fish muscle were obtained, respectively, for mullet packaged under modified atmosphere and air. Sensory analyses (odor and taste attributes) showed that the limit of sensorial acceptability was reached after ca. 6 days for the samples packaged in air, 8 days for the M1 and M3 samples, and after 10 days for the M2 samples. Respective shelf-life extension for fresh whole mullet was ca. 2 days (M1 and M3 gas mixtures), and 4 days (M2 gas mixture).     

Exploration of economical sizing of gas engine and thermal store for combined heat and power plants in the UK

There has been discussion about the extent to which combined heat and power (CHP) plants with thermal stores are suitable for sustainable energy production. At the moment, in the UK the development of this type of plant is limited. This paper analyses the economics and optimum size of CHP operating with gas engines and thermal stores in British market conditions. This is achieved using energyPRO software. It is shown that, due to the big differences in electricity prices between day and night, the use of thermal stores could be profitable in the UK. The economical size of CHP plant for a district or community heating load of 20,000 MWh per year is found to be a 3 MWe gas engine with a 7.8 MWh thermal store. In this case the analysis reveals that the use of a thermal store more than doubles the return on investments (as measured in net present value) compared with the same size of a plant without a thermal store. It is concluded that thermal stores can improve the overall economics of CHP plants in present British circumstances.     

Fish industry waste: treatments, environmental impacts, current and potential uses

Fish waste management has been one of the problems having the greatest impact on the environment. Fish farming detrimental effects on the marine environment in particular have become an issue of public concern. In European Union, numerous Directives, Decisions and Regulations were voted in an attempt to minimise the environmental impact of fisheries within the frame of Integrated Coastal Management. Treated fish waste has found many applications among which the most important are animal feed, biodiesel/biogas, dietic products (chitosan), natural pigments (after extraction), food‐packaging applications (chitosan), cosmetics (collagen), enzyme isolation, Cr immobilisation, soil fertiliser and moisture maintenance in foods (hydrolysates). In this review, an update of both environmental impact (inputs and outputs) and treated fish waste uses is provided by means of six comprehensive tables and seven figures.     

Intrusion detection in MANET using classification algorithms: The effects of cost and model selection

Intrusion detection is frequently used as a second line of defense in Mobile Ad-hoc Networks (MANETs). In this paper we examine how to properly use classification methods in intrusion detection for MANETs. In order to do so we evaluate five supervised classification algorithms for intrusion detection on a number of metrics. We measure their performance on a dataset, described in this paper, which includes varied traffic conditions and mobility patterns for multiple attacks. One of our goals is to investigate how classification performance depends on the problem cost matrix. Consequently, we examine how the use of uniform versusweighted cost matrices affects classifier performance. A second goal is to examine techniques for tuning classifiers when unknown attack subtypes are expected during testing. Frequently, when classifiers are tuned using cross-validation, data from the same types of attacks are available in all folds. This differs from real-world employment where unknown types of attacks may be present. Consequently, we develop a sequential cross-validation procedure so that not all types of attacks will necessarily be present across all folds, in the hope that this would make the tuning of classifiers more robust. Our results indicate that weighted cost matrices can be used effectively with most statistical classifiers and that sequential cross-validation can have a small, but significant effect for certain types of classifiers.     

Restaurierung antiker Tempel: Experimentelle Untersuchungen zum Ausziehverhalten von Verankerungen im Marmor

Restoration of antique temples: Experimental investigations on the pull‐out behaviour of anchors in marble. In restoration works of antique Greek temples individual marble blocks are connected together to form a single element, e.g. an architrave. Due to the demand of reversibility of the intervention, this is done in the Acropolis of Athens by means of titanium threaded bars that are inserted in the marble, in predrilled holes filled with cement mortar. This way of restoration corresponds to a partial rehabilitation of the architraves. This paper presents experimental investigations on the pull‐out behaviour of such anchors. The tests are performed in specially designed equipment. Failure takes place for all tests in the interface between the mortar and the marble. It has been shown that the form of the thread influences considerably the carrying capacity. Possible limit states are introduced.     

Microbial spoilage analysis and its effect on chemical changes and shelf‐life of Norway lobster (Nephrops norvegicus) stored in air at various temperatures

Microbial activity and spoilage of Norway lobster (Nephrops norvegicus) from Greek temperate waters, stored in air at 20, 5 and 0 °C was assessed. Microbial spoilage population, Total Volatile‐Base Nitrogen (TVB‐N), Trimethylamine‐Nitrogen (TMA‐N), pH and organoleptic changes were determined. Shelf‐life of Norway lobster stored at 20, 5 and 0 °C was 24, 72 and 96 h respectively. The product was spoiled due to the development of TVB‐N and melanosis of the carapace, as TVB‐N was apparent from the early stages of storage and correlated very well with microbial growth and shelf‐life. Pseudomonas sp. was the predominant spoilage bacterium. Product rejection coincided with TVB‐N levels of 330–400 mg kg^?1 and Pseudomonas sp. population level of about 5 × 10⁵ cfu g^?1. Pseudomonas sp. and TVB‐N are presumably the Specific Spoilage Organism and the Chemical Spoilage Index of the product.     

Multiplayer online games as educational tools: Facing new challenges in learning

This paper outlines a proposal for the development of educational multiplayer online games based on the activity theory, as an alternative to the current trend in multiplayer gaming and a means of promoting collaboration among students. In order to examine whether online games are engaging for learners, we consider multiple factors regarding game play – such as frequency of game use, gender differences, identification with the characters, and game preferences – as well as some psychosocial factors that may influence learning – such as academic performance, self-esteem, and computer self-efficacy. This paper suggests that online multiplayer educational games should be approached as a complex learning system, based on the principles of activity theory, where the Subjects would interact with other Subjects, Objects and Tools of the game, under specified Rules and create Communities through division of labor, leading to the expected learning outcome. Thus, we suggest taking into account some important issues concerning the Subjects that the activity theory refers to, such as gender differences in playing games, academic performance, self-esteem and computer self-efficacy.     

Salinity Stress Alters the Secondary Metabolic Profile of M. sativa,M. arborea and Their Hybrid (Alborea)

Increased soil salinity, and therefore accumulation of ions, is one of the major abiotic stresses of cultivated plants that negatively affect their growth and yield. Among Medicago species, only Medicago truncatula, which is a model plant, has been extensively studied, while research regarding salinity responses of two important forage legumes of Medicago sativa (M. sativa) and Medicago arborea (M. arborea) has been limited. In the present work, differences between M. arborea, M. sativa and their hybrid Alborea were studied regarding growth parameters and metabolomic responses. The entries were subjected to three different treatments: (1) no NaCl application (control plants), (2) continuous application of 100 mM NaCl (acute stress) and (3) gradual application of NaCl at concentrations of 50-75-150 mM by increasing NaCl concentration every 10 days. According to the results, M. arborea maintained steady growth in all three treatments and appeared to be more resistant to salinity. Furthermore, results clearly demonstrated that M. arborea presented a different metabolic profile from that of M. sativa and their hybrid. In general, it was found that under acute and gradual stress, M. sativa overexpressed saponins in the shoots while M. arborea overexpressed saponins in the roots, which is the part of the plant where most of the saponins are produced and overexpressed. Alborea did not perform well, as more metabolites were downregulated than upregulated when subjected to salinity stress. Finally, saponins and hydroxycinnamic acids were key players of increased salinity tolerance.     

Customer engagement and performance in social media: a managerial perspective

Electronic Markets - The topic of customer engagement via social media is receiving increasing consideration in the literature. Previously, scholars’ use of the notion and dimensionality of...