Exact two-dimensional wave-front reconstruction from lateral shearing interferograms with large shears

A method is proposed for exact discrete reconstruction of a two-dimensional wave front from four suitably designed lateral shearing experiments. The method reconstructs any wave front at evaluation points of a circular aperture exactly up to an arbitrary constant for noiseless data, and it shows excellent stability properties in the case of noisy data. Application of large shears is allowed, and high resolution of the reconstructed wave front can be achieved. Results of numerical experiments are presented that demonstrate the capability of the method.     

Thermal emission from dielectric beam splitters in michelson interferometers: a schematic analysis

Thermal emission from beam splitters in Fourier transform infrared spectrometers causes spectral amplitudes that are in quadrature to those of radiation from the field of view or from the detector port. Beam-splitter emission is described as a superposition of radiation of electromagnetic dipoles with angular polarization correlation taken into account for the real refractive index. Surface emission shows characteristic differences compared with volume emission. Numerical data are given for experimental conditions adapted to those of the airborne limb sounder MIPAS-FT.     

Nonlinear rotation-invariant pattern recognition by use of the optical morphological correlation

We introduce a modification of the nonlinear morphological correlation for optical rotation-invariant pattern recognition. The high selectivity of the morphological correlation is conserved compared with standard linear correlation. The operation performs the common morphological correlation by extraction of the information by means of a circular-harmonic component of a reference. In spite of some loss of information good discrimination is obtained, especially for detecting images with a high degree of resemblance. Computer simulations are presented, as well as optical experiments implemented with a joint transform correlator.     

Formate protects photosynthetic machinery from photoinhibition

In plants, environmental adversity often leads to the formation of reactive oxygen species (ROS) because of excess light energy. We found that incubation of rice leaves under high light intensity together with CO2-depleted air seriously damaged photosynthesis; however, the application of potassium formate (2 mM) before the photoinhibitory treatment protected the photosynthesis of the plant from photoinhibition. Further analysis revealed that formate protects PSII, RuBisCO, and FBPase from photoinhibition. Formate is thought to be involved in endogenous radical scavenging and/or in the supply of CO2, derived from the formate, thereby reducing oxidative damage to the photosystems under photoinhibitory conditions.     

Effect of misidentification on genetic gain and estimation of breeding value in dairy cattle populations

The effect of pedigree errors on estimated breeding value and genetic gain for a sex-limited trait with heritability of 0.25 was evaluated. Ten populations of 100,000 milking cows were simulated with correct paternity identification for all animals, and 10 populations were simulated with 10% incorrect paternal identification. The initial populations consisted of 100,000 unrelated individuals, and simulations were continued for 20 yr. The BLUP genetic evaluations were computed every year by an animal model analysis for each complete population. Estimated breeding values for the populations with 10% incorrect paternity were biased, especially in the later generations. Genetic gains were 4.3% higher with correct paternity identification. Reduction of pedigree errors by paternity confirmation of daughters of test sires by DNA microsatellites may result in considerable economic benefits, depending on the cost of testing in each country.     

Trans fatty acids in adipose tissue of French women in relation to their dietary sources

This study reports the fatty acid composition of subcutaneous adipose tissue in French women with special emphasis on the content of trans fatty acids originating from two main dietary sources, ruminant fats and partially hydrogenated vegetable oils (PHVO). Adipose tissue trans fatty acid levels from 71 women, recruited between 1997 and 1998, were determined using a combination of capillary gas chromatography and silver nitrate thin-layer chromatography. Results indicate that on average cis monounsaturates accounted for 47.9% of total fatty acids, saturates for 32.2%, and linoleic acid for 14.4%. Cis n−3 polyunsaturates represented only 0.7%. Total content of trans fatty acids was 2.32±0.50%, consisting of trans 18∶1 (1.97±0.49%), trans 18∶2 (0.28±0.08%), and trans 16∶1 (0.06±0.03%). Trans 18∶3 isomers were not detectable. The level of trans fatty acids found in adipose tissue of French women was lower than those reported for Canada, the United States, and Northern European countries but higher than that determined in Spain. Therefore, trans fatty acid consumption in France appears to be intermediate between that of the United States or North Europe and that of Spain. Based on the equation of Enig et al., we estimated the mean daily trans 18∶1 acid intake of French women at 1.9 g per person. The major trans 18∶1 isomer in adipose tissue was Δ11trans, as in ruminant fats. Estimates of relative contribution of trans fatty acid intake were 55% from ruminant fats and 45% from PHVO. This pattern contrasts sharply with those established for Canada and the United States where PHVO is reported to be the major dietary source of trans fatty acids.     

Evaluation of biohazards in dehydrated biofilms on foodstuff packaging

Plastic materials used for food packaging are clean but not sterile when the food is just packaged. Accidental wet contamination may occur at every moment between packaging and opening by the consumer: on polyethylene (PET), bacteria may adhere strongly and constitute a biofilm in less than 24 h. By rolling on themselves, PET sheets may contaminate food. We tried to show that contact with salted foodstuffs favoured microbial recovery. Four strains were chosen to perform biofilms on PET: Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli. Biofilms were dried up 24 h. Biofilm bacteria were stressed by adhesion, by starvation and by dehydration. However, they were capable of recovery in salted solutions or media, probably because one (or more) stress protected them against another stress. Stress was demonstrated by stress protein production, by mean of electrophoresis, and membrane lesions by mean of flow cytometry. Stress recovery was performed in aqueous salted solutions or salted brain-heart infusion with NaCl 9, 15, 20 and 30 g/l. Staphylococci were more sensitive to these stresses and recovery was a function of salt concentration. Gram-negative bacteria were little affected by stresses; salt effects were less important. If all these biofilms were capable of recovery from stresses in salted media, flexible PET could possibly lead to a health hazard when it is used for wet salt meats, e.g.     

Proteins variations in Listeria monocytogenes exposed to high salinities

Listeria monocytogenes Scott A grown in the minimal chemically defined medium M6LT was challenged to a concentration of either 35 or 65 g l(-1) of NaCl for 1 h in the presence of a [35S]cysteine-[35S]methionine labelling mix. The protein patterns were analysed by 2D-electrophoresis in the two conditions and isoosmotic condition (5 g l(-1) of NaCl in M6LT). A great number of proteins which were synthesized under isoosmotic conditions were either completely repressed or expressed at a reduced level, at 65 g l(-1) and to a lesser extent at 35 g l(-1) of NaCl. At 35 g l(-1) of NaCl, six proteins were up-regulated, five proteins showed no change in expression level and five were repressed. Among the proteins up-regulated at 35 g l(-1) of NaCl, a single one (18.7 kDa, pI 5.05) was up-regulated at 65 g l(-1) too. We observed 21 proteins which were repressed at 65 g l(-1) of NaCl, among which 11 completely disappeared. Some of the up-regulated proteins have characteristics of molecular weight and isoelectric point close to those of stress proteins reported elsewhere: the protein induced both at 35 and 65 g l(-1) might correspond to a previously proposed universal stress protein of Listeria. Some proteins which were repressed at 65 g l(-1) have molecular weights close to those of virulence proteins.     

Automatic on-line coupling of supercritical fluid extraction and capillary electrophoresis

An interface for the automatic coupling of a supercritical fluid extractor (SFE) with capillary electrophoretic (CE) equipment, both commercially available, was developed with a view to improving sample treatment, which is a crucial step in capillary electrophoresis. Extracted analytes were collected in a trap following depressurization in the SFE and were transferred to the CE equipment across the interface. The key elements of the experimental assembly are a laboratory-made programmable arm and the autosampler of the CE equipment, both of which are controlled by a built-in microprocessor using an appropriate electronic interface and customized software. This combined system was successfully used to determine cresols and chlorophenols in liquid samples (river water and human urine) with increased precision, throughput, and automatability. The proposed arrangement opens up interesting prospects for the direct determination of analyte traces in solid samples without human intervention.