低成本微反应槽PCR芯片阵列双重控制系统

设计了内部不含温度传感元件、加热元件的低成本微反应槽聚合酶链式反应（PCR）芯片．研制了宏观集中控制与微观分散控制有机结合的双重控制系统,该系统具备对PCR芯片的批量处理能力;宏观集中控制装置以水为传热媒质通过特制换热器给芯片提供聚合酶反应所需的基本温度;在柔性印刷电路板上形成与芯片阵列对应的微型加热器阵列,针对各芯片进行分散的温度补偿。     

壳聚糖与芫荽精油复合涂膜在草莓常温贮藏中的应用

该研究以芫荽精油与1%的壳聚糖复合制备不同浓度的保鲜液,对新鲜且品质优良的草莓进行浸泡涂膜保鲜,以超纯水为对照组,通过测定贮藏期间草莓的感官品质以及各理化指标,探究该复合保鲜液对草莓常温贮藏效果的影响。结果表明,与纯水组相比芫荽精油与壳聚糖复合涂膜有利于草莓保鲜,且精油浓度越高保鲜效果也越好。在贮藏第8 天时,芫荽精油浓度为0.18%的保鲜组草莓腐烂率低于30%,失重率、丙二醛含量分别比对照组低62.5%和39.7%,VC 含量与可滴定酸含量分别是对照组的1.9、2.1 倍,差异显著,表明较高浓度的芫荽精油涂膜有助于草莓保持良好的感官品质和营养价值。     

南京市南河初雨调蓄池工艺设计

随着径流污染对水体水质影响的日益加重,初雨调蓄池作为一种行之有效的方式在国内逐渐得到应用.结合南京市南河初雨调蓄池的设计实例,介绍了调蓄池规模、选址、工艺设计及运行模式等要点,并通过优化相关控制系统的组合方式,充分发挥工程效益.根据实际运行效果,初雨调蓄池的建设不仅削减了入河的初雨量,同时明显改善南河水质.     

改性淀粉-丙烯酰胺接枝共聚调堵剂的动态成胶性能

深部封堵技术是改善窜流型油藏开发效果的关键技术之一。改性淀粉-丙烯酰胺接枝共聚体系是新型的聚合物凝胶调堵剂。采用30m超长填砂管,模拟了吉林扶余油田的基质和高渗透条带,研究了这种调堵剂在油藏运穆过程中的动态成胶性能。研究结果表明,该调堵剂在运移过程中仍能形成凝胶,其初始成胶时间与在静态条件下基本相同,但完全成胶时间比在静态条件下长;调堵剂完全成胶后,具有很强的封堵能力,封堵系数高达30000。     

陆相断陷盆地地层油藏勘探现状与研究方向——以济阳坳陷为例

陆相断陷盆地多期成盆、凹凸相间和多元成烃的地质特征决定了地层油藏在其中广泛发育的可能性。济阳坳陷作为陆相断陷盆地的典型代表，地层油藏的勘探经历了偶然发现、勘探突破和持续发展3个阶段，已发现的第三系地层油藏主要富集于靠近洼陷10km以内的凸起周缘鼻状构造带，其形成主要受16种不整合构建样式和断层-砂体-不整合面运聚系统的控制。在陆相断陷盆地地层油藏形成条件、济阳坳陷第三系地层油藏勘探现状及形成机制的研究基础上，结合调研结果，提出地层油藏攻关研究的3个主要方向：①不整合构建样式及展布与油气分布关系静态模型；②参地层油藏形成过程与成藏模式；③地层圈闭准确描述技术。最后，提出了解决这些问题的技术思路和研究手段。     

Selection of RNA-Cleaving TNA Enzymes for Cellular Mg2+ Imaging

Catalytic DNA-based fluorescent sensors have enabled cellular imaging of metal ions such as Mg²⁺. However, natural DNA is prone to nuclease-mediated degradation. Here, we report the in vitro selection of threose nucleic acid enzymes (TNAzymes) with RNA endonuclease activities. One such TNAzyme, T17–22, catalyzes a site-specific RNA cleavage reaction with a k_cat of 0.017 min⁻¹ and K_M of 675 nM. A fluorescent sensor based on T17–22 responds to an increasing concentration of Mg²⁺ with a limit of detection at 0.35 mM. This TNAzyme-based sensor also allows cellular imaging of Mg²⁺. This work presents the first proof-of-concept demonstration of using a TNA catalyst in cellular metal ion imaging.     

Differences in Oxidative Stability,Sensory Properties,and Volatile Compounds of Pepper Aromatized Sunflower Oils Prepared by Different Methods during Accelerated Storage

The study aims to compare the differences in the oxidative stability, sensory properties, and volatile compounds during accelerated storage of pepper aromatized sunflower oil (PASO) samples prepared by maceration method (PASO-M), co-pressing method (PASO-C), and direct addition method (PASO-A), respectively. The results exhibit that their oxidative stability is in the relative order: PASO-A > PASO-C > PASO-M. Meanwhile, the PASO-A sample is the most preferable aromatized sunflower oil by consumers according to sensory analysis. In addition, a total of 83 volatile compounds are identified in the three PASO samples during the storage, and the principal component analysis (PCA) shows that their volatile compounds are quite different at the initial stage of the storage. Still, they are similar among each other from the middle of storage period. As consequence, the aromatized sunflower oil sample prepared by the direct-addition method during the storage possesses better oxidative stability and sensory attributes, and there is not much difference among their volatile compounds, indicating that the aromatized sunflower oil prepared by direct-addition method can be developed as promising aromatized sunflower oil. Practical applications: This study shows that aromatization increases oxidative stability and sensory properties of sunflower oil. By this way some sunflower oils with special sensory properties are obtained: special smell and taste, special color, etc. These peppers aromatized sunflower oils can be used to increase taste and smell in some dishes. For example, they can be used in some salads and some roast meats to give more taste and color.     

植物功能性食品在促进健康和预防疾病中的作用

对一些常见的植物功能性食物在疾病预防和促进健康中的作用及其功能性因子的研究现状进行了综述。     

运用排替压力法定量预测断层侧向封闭能力——以济阳坳陷为例

断层对油气的封闭作用主要体现在侧向封闭上,而断层岩的排替压力是评价断层侧向封闭的主要参数,泥质含量和埋深则是影响岩石排替压力的主要因素.断层岩的泥质含量或埋深越大,其排替压力越大,对油气的封堵能力越强.通过实验测试手段可以建立不同泥质含量断层岩的排替压力与埋深的关系,从而可以实现对断层岩排替压力的预测.断层岩与储层的排...