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91.
Enterobacteria on modified-atmosphere (MA) packaged meat (n = 54) and poultry (n = 32) products were enumerated, and 899 isolates were picked and ribotyped. For identification, 16S rRNA genes of representative strains were sequenced and analyzed. Altogether 54 (60%) of the samples contained enterobacteria >104 CFU/g. In 34% of the poultry samples, enterobacteria counts were >106 CFU/g suggesting that enterobacteria may contribute to spoilage of MA packaged poultry. The enterobacteria identified were predominantly Hafnia spp. (40%) and Serratia spp. (42%) with Hafnia alvei, Hafnia paralvei, Serratia fonticola, Serratia grimesii, Serratia liquefaciens, Serratia proteamaculans, and Serratia quinivorans being the species identified. In addition, 6% of the isolates were identified as Rahnella spp., 3% as Yersinia spp., and 1% as Buttiauxella spp. Percentage distributions of the predominant genera in different products showed that 89% of the Serratia spp. were from products packaged under a high-O2 MA containing CO2 (25–35%), whereas most (76%) isolates of Hafnia originated from anaerobically packaged red meat and poultry. These findings suggest that the gas mixture used for MA packaging influence the selection of enterobacteria growing on meat and poultry. 相似文献
92.
Elina Siirola Francesco G. Mutti Barbara Grischek Sebastian F. Hoefler Walter M. F. Fabian Gideon Grogan Wolfgang Kroutil 《Advanced Synthesis \u0026amp; Catalysis》2013,355(9):1703-1708
Prochiral bicyclic diketones were transformed to a single diastereomer of 3‐substituted cyclohexylamine derivatives via three consecutive biocatalytic steps. The two chiral centres were set up by a C C hydrolase (6‐oxocamphor hydrolase) in the first step and by an ω‐transaminase in the last step. The esterification of the intermediate keto acid was catalysed by a lipase in the second step if possible. For two substrates the C C hydrolytic step as well as the esterification could be run simultaneously in a one‐pot cascade in an organic solvent. In one example, the reaction mixture of the first two steps could be directly subjected to bio‐amination in an organic solvent without the need to change the reaction medium. Depending on the choice of the ω‐transaminase employed and the substrate the cis‐ as well as the trans‐diastereomers could be obtained in optically pure forms. 相似文献
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The performance of a novel thermal swing sorption-enhanced reaction (TSSER) concept for simultaneous production of fuel-cell grade hydrogen and compressed carbon dioxide as a by-product from a synthesis gas feed is simulated using Na2O promoted alumina as a CO2 chemisorbent in the process. The process simultaneously carries out the water gas shift (WGS) reaction and removal of CO2 from the reaction zone by chemisorption in a single unit. Periodic regeneration of the chemisorbent is achieved by using the principles of thermal swing adsorption employing super-heated steam purge. 相似文献
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Lack of male teachers: a problem for students or teachers? 总被引:5,自引:0,他引:5
Elina Lahelma 《Pedagogy, Culture & Society》2000,8(2):173-186
This article explores young people's perspectives on males and females as teachers, contrasting these with teachers' perceptions. It builds on 90 interviews of school students aged 13-14 and 60 follow-up interviews 4 years later. The first interviews were conducted in ethnographic context in two secondary schools in the mid-1990s in Helsinki, Finland. Whilst lack of male teachers is a recurrent theme in educational discussion, widely agreed among teachers, gender did not appear to be relevant when young persons talked about teachers. They appreciate teachers, irrespective of gender, who can teach and are friendly and relaxed, but who nevertheless keep order and make sure that students work. Male teachers who teach popular, non-academic subjects were often favoured by boys, but so were female teachers of academic subjects and increasingly as time went on. The interviews suggest that students do not need male teachers to act as 'male models'. They also suggest that male teachers should be sensitive in relationships with female students. When questioned explicitly, most of the interviewees did not regard lack of male teachers as a major problem 相似文献
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Salvador Camilo A. F. Dal Bo Mariana R. Lima Dalton D. Miranda Caetano R. Caram Rubens 《Journal of Materials Science》2021,56(19):11494-11510
Journal of Materials Science - Among many β-metastable alloys explored for biomedical applications, alloys from the Ti-Nb-Fe-Zr system present great potential regarding cost and mechanical... 相似文献
100.
Christian Svio-Silva Poliana E. Soinski-Sousa Antnio Simplício-Filho Rosana M. C. Bastos Stephany Beyerstedt rika Bevilaqua Rangel 《International journal of molecular sciences》2021,22(4)
Diabetic kidney disease (DKD) is a worldwide microvascular complication of type 2 diabetes mellitus (T2DM). From several pathological mechanisms involved in T2DM-DKD, we focused on mitochondria damage induced by hyperglycemia-driven reactive species oxygen (ROS) accumulation and verified whether mesenchymal stem cells (MSCs) anti-oxidative, anti-apoptotic, autophagy modulation, and pro-mitochondria homeostasis therapeutic potential curtailed T2DM-DKD progression. For that purpose, we grew immortalized glomerular mesangial cells (GMCs) in hyper glucose media containing hydrogen peroxide. MSCs prevented these cells from apoptosis-induced cell death, ROS accumulation, and mitochondria membrane potential impairment. Additionally, MSCs recovered GMCs’ biogenesis and mitophagy-related gene expression that were downregulated by stress media. In BTBRob/ob mice, a robust model of T2DM-DKD and obesity, MSC therapy (1 × 106 cells, two doses 4-weeks apart, intra-peritoneal route) led to functional and structural kidney improvement in a time-dependent manner. Therefore, MSC-treated animals exhibited lower levels of urinary albumin-to-creatinine ratio, less mesangial expansion, higher number of podocytes, up-regulation of mitochondria-related survival genes, a decrease in autophagy hyper-activation, and a potential decrease in cleaved-caspase 3 expression. Collectively, these novel findings have important implications for the advancement of cell therapy and provide insights into cellular and molecular mechanisms of MSC-based therapy in T2DM-DKD setting. 相似文献