Microbiological Quality of Raw Dried Pasta from the German Market,with Special Emphasis on Cronobacter Species

The microbiological quality of 132 dried pasta products available on the German market, originating from 11 different countries, was studied. Sample materials included soft or durum wheat products, some of which produced with other ingredients such as eggs, spices, or vegetables. Parameters included hygiene indicators (aerobic plate count, mold count, the presence of Enterobacteriaceae) and pathogenic/toxinogenic bacterial species (Salmonella spp., Staphylococcus aureus, presumptive Bacillus cereus, and Cronobacter spp.). The overall results of hygiene parameters indicated a satisfactory quality. Salmonella was not found in any sample. Three samples were positive for S. aureus (10² to 10⁴ colony forming unit (CFU)/g). Presumptive B. cereus at levels of 10³ to 10⁴ CFU/g were detected in 3 samples. Cronobacter spp. were isolated from 14 (10.6%) products. Of these, 9 isolates were identified as C. sakazakii, 2 each as C. turicensis and C. malonaticus, and 1 as C. muytjensii. The isolates were assigned to 9 multilocus sequence typing (MLST) sequence types and to 14 different PFGE profiles. Although pasta products are typically cooked before consumption, some consumers, and children in particular, may also eat raw pasta as nibbles. Raw pasta seems to be a relevant source of exposure to dietary Cronobacter spp., although health risks are probably restricted to vulnerable consumers. High numbers of presumptive B. cereus as found in some samples may be a risk after improper storage of cooked pasta products because toxinogenic strains are frequently found within this species.     

Pre‐ or post‐harvest applications of putrescine and low temperature storage affect fruit ripening and quality of ‘Angelino’ plum

BACKGROUND: Plum has a very short storage life. The role of pre‐ or post‐harvest applications of putrescine (PUT) and low temperature storage on fruit ripening and quality was investigated in plum fruit (Prunus salicina Lindl. cv. Angelino). RESULTS: Pre‐ or post‐harvest PUT treatments [(0.1, 1.0 or 2.0 mmol L^?1) + 0.01% Tween‐20 as a surfactant] delayed and suppressed the climacteric ethylene production and respiration rate irrespective of the method used to apply PUT. PUT‐treated fruit following low temperature storage (0 ± 1 °C; 90 ± 5% RH), at the ripe stage exhibited higher fruit firmness and titratable acidity (TA), while soluble solids content (SSC), levels of ascorbic acid, total carotenoids and total antioxidants were lower than in untreated fruit. Fruit both sprayed with PUT and stored in low temperature for 6 weeks, at the ripe stage showed reduced respiration rate, delayed changes in the SSC:TA ratio and levels of total carotenoids compared to post‐harvest PUT application. CONCLUSION: Pre‐harvest application of 2.0 mmol L^?1 PUT 1 week before the anticipated commercial harvest was more effective in delaying plum fruit ripening and can be used to extend the storage (0 ± 1 °C) life of plums for up to 6 weeks with minimum losses in fruit quality. Copyright © 2008 Society of Chemical Industry     

SGLT2 Inhibitors and Their Antiarrhythmic Properties

Sodium-glucose cotransporter 2 (SGLT2) inhibitors are gaining ground as standard therapy for heart failure with a class-I recommendation in the recently updated heart failure guidelines from the European Society of Cardiology. Different gliflozins have shown impressive beneficial effects in patients with and without diabetes mellitus type 2, especially in reducing the rates for hospitalization for heart failure, yet little is known on their antiarrhythmic properties. Atrial and ventricular arrhythmias were reported by clinical outcome trials with SGLT2 inhibitors as adverse events, and SGLT2 inhibitors seemed to reduce the rate of arrhythmias compared to placebo treatment in those trials. Mechanistical links are mainly unrevealed, since hardly any experiments investigated their impact on arrhythmias. Prospective trials are currently ongoing, but no results have been published so far. Arrhythmias are common in the heart failure population, therefore the understanding of possible interactions with SGLT2 inhibitors is crucial. This review summarizes evidence from clinical data as well as the sparse experimental data of SGLT2 inhibitors and their effects on arrhythmias.     

Group ICA of resting-state data: a comparison

Objective

Independent component analysis (ICA) has proven its applicability in both standard and resting-state fMRI. While there is consensus on single-subject ICA methodology, the extension to group ICA is more complex and a number of approaches have been suggested. Currently, two software packages are most frequently used for ICA group analysis: (1) GIFT introduced by Calhoun et al. [7], and (2) PICA, proposed by Beckmann et al. [3]. Both methods are based on the assumption of statistical independence of the extracted component maps (“spatial ICA”). Group maps are estimated via ICA on pre-calculated group data sets.

Material and Methods

In this study, we applied the two analysis approaches to a group of fMRI resting-state data sets obtained from twenty-eight healthy subjects. Default implementations were used and the number of components was restricted to 5, 10, 15, 20, 25, 30, and 35. The performance of GIFT and PICA was assessed with respect to the number of resting-state networks detected at different component estimation levels and computational load.

Results

At low component estimation levels GIFT analysis resulted in more RSNs than PICA, while for individually determined component levels both approaches obtained the same RSNs. Although component maps show some variability across the two methods, spatial and temporal comparison using correlation coefficients resulted in no significant differences between the RSNs detected across the different analyses

Conclusion

Our results show that both approaches provide an adequate way of group ICA obtaining a comparable number of RSNs differing mainly in calculation times.