Three-Dimensional Cell Culture: A Breakthrough in Vivo

Cell culture is an important tool for biological research. Two-dimensional cell culture has been used for some time now, but growing cells in flat layers on plastic surfaces does not accurately model the in vivo state. As compared to the two-dimensional case, the three-dimensional (3D) cell culture allows biological cells to grow or interact with their surroundings in all three dimensions thanks to an artificial environment. Cells grown in a 3D model have proven to be more physiologically relevant and showed improvements in several studies of biological mechanisms like: cell number monitoring, viability, morphology, proliferation, differentiation, response to stimuli, migration and invasion of tumor cells into surrounding tissues, angiogenesis stimulation and immune system evasion, drug metabolism, gene expression and protein synthesis, general cell function and in vivo relevance. 3D culture models succeed thanks to technological advances, including materials science, cell biology and bioreactor design.     

Life cycle emissions analysis of two nZEB concepts

The net-zero emissions building (nZEB) performance is investigated for building operation (EO) and embodied emissions in materials (EE) for Norway's cold climate. nZEB concepts for new residential and office buildings are conceived in order to understand the balance and implications between operational and embodied emissions over the building's life. The main drivers for the CO₂ equivalent (CO₂e) emissions are revealed for both building concepts through a detailed emissions calculation. The influence of the CO₂e factor for electricity is emphasized and it is shown to have significant impact on the temporal evolution of the overall CO₂e emissions balance. The results show that the criterion for zero emissions in operation is easily reached for both nZEB concepts (independent of the CO₂e factor considered). Embodied emissions are significant compared to operational emissions. It was found that an overall emissions balance including both operational and embodied energy is difficult to reach and would be unobtainable in a scenario of low carbon electricity from the grid. In this particular scenario, the net balance of emissions alone is nonetheless not a sufficient performance indicator for nZEB.     

探析中国传统植物学知识

<正>约瑟夫·尼达姆[李约瑟](Joseph Needham,1900-1995)曾在《中国科学与文明史》(Science and Civilisation in China)第6卷第1期发表了对中国植物学的专项研究。由此,读者可以了解到:在中国古代医学和     

Impact of retention on trans‐column velocity biases in packed columns

The heights equivalent to a theoretical plate of a weakly and strongly retained compounds were measured on two packed columns having different average mesopore sizes. The measurements were carried out in two different cases, with access to the mesopores by the sample molecules blocked (filled with n‐nonane) or not. The experimental results demonstrate that the eddy dispersion terms of both columns are significantly smaller for porous than for nonporous particles. Two simultaneous phenomena explain this observation. First, packed columns are radially heterogeneous which causes significant trans‐column velocity biases warping the bands. Second, radial dispersion contributes to mass transfer across the column, relaxing the radial concentration gradients that are caused by these velocity biases. The impact of these biases is minimized when the pores of the particles are not blocked; it decreases with increasing residence time and radial dispersion coefficient of the solutes. © 2009 American Institute of Chemical Engineers AIChE J, 2009     

Membrane performance in combined processes including ozonation or advanced oxidation, powdered activated carbon and coagulation — Investigations in pilot scale

In Luxembourg the largest drinking water production plant treating reservoir water with conventional technology including ozonation is in operation since 1969. Its maximum capacity is 64,000 m³/d. After 40 years of operation the need for both modernizing and extension of capacity to 100,000 m³/d emerged. Nowadays, due to new developments in technology and research it is possible to assess the water treatment processes more effectively with regard to current and future requirements. Against this background, membrane processes combined with conventional and advanced processes were identified as potential option and long term pilot-scale tests were started in June 2008. Different treatment process schemes all including membranes are investigated in a one year pilot plant period. Various treatment steps such as different pre- and post-treatment steps, various membranes and membrane materials and also conventional treatment steps are compared and assessed.     

Analyzing and modeling the performance degradation of flash A/D converters due to substrate noise coupling

This paper presents a model to evaluate the impact of substrate noise on a CMOS regenerative comparator and moreover to predict the resulting performance degradation of a flash analog-to-digital (A/D) converter. The proposed approach initially relates substrate noise to the induced timing uncertainty of the comparator by means of an analytical linear model. In particular, the analysis first focuses on analyzing and expressing the resulting non-uniform sampling distortion in regenerative comparators in the presence of a deterministic ground bounce. Two sources of distortion are identified and evaluated: the input-dependent and the substrate noise-dependent one. For each error contributor, the analysis investigates two cases of timing error, based on the frequency correlation of the interfering signal with the sampling clock. The properties (number and power of distortion tones) of the sampling error spectrum are found to be highly dependent on the spectral content of the interfering signal and the sampling clock, while the model captures accurately the induced distortion. Subsequently, the linear model is extended to estimate the degradation of flash A/D converters and is utilized to predict the performance of practical flash and time-interleaved converters in the presence of substrate noise.     

Tests for the Use of La2Mo2O9‐based Oxides as Multipurpose SOFC Core Materials

The mixed ionic–electronic conductivity under dilute hydrogen, the stability and the catalytic activity under propane:air type mixtures of a series of LAMOX oxide‐ion conductors have been studied. The effect of exposure to dilute hydrogen on the conductivity of the β‐La₂(Mo_{2 – y}W_y)O₉ series at 600 °C depends on tungsten content: almost negligible for the highest (y = 1.4), it is important for La₂Mo₂O₉ (y = 0). In propane:air, all tested LAMOX electrolytes are stable at 600–700 °C, but get reduced when water vapour is present. La₂Mo₂O₉ is the best oxidation catalyst of the series, with an activity comparable to that of nickel. The catalytic activity of other tested LAMOX compounds is much lower, (La_1.9Y_0.1)Mo₂O₉ showing a deactivation phenomenon. These results suggest that depending on composition, La₂(Mo_{2 – y}W_y)O₉ compounds could be either electrolytes in single‐chamber SOFC and dual‐chamber micro‐SOFC (y = 1.4) or anode materials in dual‐chamber SOFC (low y) or oxidation catalysts in SOFCs operating with propane (y = 0).     

Hydrogen isotopic enrichment: an indicator of biodegradation at a petroleum hydrocarbon contaminated field site

Compound-specific carbon and hydrogen isotope analysis was used to investigate biodegradation of benzene and ethylbenzene in contaminated groundwater at Dow Benelux BV industrial site. delta13C values for dissolved benzene and ethylbenzene in downgradient samples were enriched by up to 2+/-0.5 per thousand, in 13C, compared to the delta13C value of the source area samples. delta2H values for dissolved benzene and ethylbenzene in downgradient samples exhibited larger isotopic enrichments of up to 27+/-5 per thousand for benzene and up to 50+/-5 per thousand for ethylbenzene relative to the source area. The observed carbon and hydrogen isotopic fractionation in downgradient samples provides evidence of biodegradation of both benzene and ethylbenzene within the study area at Dow Benelux BV. The estimated extents of biodegradation of benzene derived from carbon and hydrogen isotopic compositions for each sample are in agreement, supporting the conclusion that biodegradation is the primary control on the observed differences in carbon and hydrogen isotope values. Combined carbon and hydrogen isotope analyses provides the ability to compare biodegradation in the field based on two different parameters, and hence provides a stronger basis for assessment of biodegradation of petroleum hydrocarbon contaminants.     

Barium Borosilicate Sealing Glasses Synthesized by a Sol–Gel Process: Chemical Interactions with a Stainless Steel and Gas‐Tightness of a SOFC

Several glasses synthesized by sol–gel route and based on the BaO–B₂O₃–X–Al₂O₃–SiO₂ (X = CaO, MgO) glass system have been investigated to evaluate their applicability as sealant for solid oxide fuel cell (SOFC). Chemical interactions with K41X stainless steel and hydrogen‐tightness of these materials were evaluated after operations at high temperatures over 1,000 h in air atmosphere. Formation of a new phase at the steel–glass interface and formation of porosity in the glass were observed and determined as critical problems over mid‐term operations. The role of MgO is important to obtain a gas‐tight sealing. Application of the glass paste without binder addition was performed in order to avoid possible residual porosity related problems. The best glass was finally used as sealant between anodic and cathodic compartments in complete SOFCs operated at 760 and at 800 °C. Open circuit voltages and power densities of the cells were recorded during the first hours of operation.