A novel peptide called Noxiustoxin 2 (NTX2) was purified from the venom of the scorpion Centruroides noxius and characterized chemically and functionally. It is composed of 38 amino acid residues linked by three disulfide bridges and its primary structure is 61% identical to that of Noxiustoxin (NTX). It is not toxic to mice (using up to 200 micrograms/20 g mouse weight) and crustaceans (up to 30 micrograms/g of crayfish), but has a paralysing effect on crickets (30 micrograms/g animal). It displaces the binding of [125I]NTX to rat brain synaptosome membranes with a Ki of 0.1 microM, in comparison NTX has a Ki of 100 pM. Similarly, using single Ca2+ activated K+ channels of small conductance obtained from cultured bovine aortic endothelial cells it was shown that NTX2 is over two logarithm units less potent than NTX in producing 50% blockade of the probability of opening the channels. NTX2 is not recognized by a panel of six distinct monoclonal antibodies against NTX, however it is recognized by polyclonal antibodies raised in mouse, with native NTX. Primary structure comparison of both NTX and NTX2 suggests that the N-terminal segments of these peptides are important for channel affinity. 相似文献
In this work, a comparative study on the combined effects of hydrodynamics and kinetics on the hydrodesulfurization (HDS) of a straight-run gas oil (SRGO) using a trickle-bed reactor with and without diluent (SiC) and different catalyst volume (0.1, 0.07, and 0.050 × 10-3 m3, respectively) was carried out. The tests were conducted in a pilot plant under conditions close to those used in industrial-scale units (5492 kPa, 445.4 m3(STP)/m3 H2/oil ratio, 623-643K, 0.9 and 2.5 h-1 LHSV values). The catalyst used was a commercial CoMoP/Al2O3 formulation shaped as tri-lobed extrudates (1/20 in, nominal size) or as crushed particles (dp = 1.2 × 10-3 m). Contrarily to that usually claimed, the undiluted systems showed better performance than those comprising small diluent (SiC) particles (dp ~ 5 × 10-5 m). Calculations to evaluate hydrodynamic variables (plug-flow behavior, wall effects, wetting, and back-mixing) were carried out in order to explain the observed facts. The possible influences of the size of the diluent particles used are discussed. 相似文献
In this work, the pentalithium ferrite was synthetized by solid-state method, and it was characterized by XRD and N2 adsorption–desorption techniques. Then, H2 production was obtained through a catalytic conversion process; chemical looping partial oxidation (CLPO) of methane using Li5FeO4 as multifunctional material. The catalytic decomposition of methane is an easy way to obtain clean energy, such as hydrogen, but in this process carbon deposition is also possible. The results showed that this material has the ability to convert methane to hydrogen, but it is also capable to donate oxygen atoms from its crystalline network, producing carbon monoxide and/or carbon dioxide, limiting the carbon deposition on the ceramic surface. In addition, it was demonstrated that this lithium-based ceramic produces hydrogen over a wide temperature range (550–900 °C), with a stable hydrogen production for 3 h at 825 °C. Furthermore, it was possible to achieve a cyclic test of methane decomposition with a pre-oxidation process between each cycle obtaining an outstanding increase in hydrogen production from 10% in cycle 1 to ~?100% in the cycle 10. This previous stage not only induces an increase in the decomposition of methane, but also avoids carbon deposition accompanied by the production of both COX compounds. Finally, it must be mentioned that Li5FeO4 is capable to chemisorb both carbon oxides produced, promoting high purity hydrogen production.
Biomaterials that meet the requirements to stimulate bone tissue formation play a vital role in orthopedics and dentistry. In this work, chitosan and a biphasic, non-cytotoxic material hydroxyapatite/whitlockite were obtained from natural sources, which are available as organic waste. The osteogenic activity was assessed using a rabbit model animal with a chitosan barrier membrane in combination with a bone-filling graft substitute composed of hydroxyapatite/whitlockite. FT-IR results showed the typical absorption bands of the chitosan and hydroxyapatite. Moreover, the X-ray diffraction pattern revealed a typical hexagonal phase of hydroxyapatite and rhombohedral structures related to whitlockite. Masson's trichrome stain showed an early formation of extracellular matrix mineralized, in accord with the surface morphology of a cortical mature bond observed by Scanning Electron Microscopy. The immunocytochemistry results showed a significant increase of positive immunoreactive cells to osteonectin in the treated defects in comparison with the control defects 6 and 8 weeks postoperatively. Overall, the results confirm that the use of this low-cost and versatile biomaterial as a barrier membrane and a bone substitute graft are useful for bone tissue engineering. 相似文献
Wastewater treatment consists of three or four sequential stages: preliminary, primary, secondary, and tertiary. Each stage can comprise multiple alternative technologies that can perform the same tasks with different efficiencies, operating times, and costs. Thus, we propose a systematic approach for designing wastewater treatment networks by utilizing principles of mathematical modeling and generating an exhaustive enumeration of all the possible technologies and their connections during the early stages of designing a treatment facility. Some of these structures are nonintuitive and include recycling, reprocessing, bypasses, and multiple technologies in parallel or series to remove the same contaminant. The nonintuitive structures with multiple technologies may provide a measure of resilience compared to typical heuristic designs. Thus, the combination of P-graph methodology and the sequence of treatment technologies predicted via the optimization algorithm from the maximal structure is based on holistic considerations and does not lead to suboptimal solutions. 相似文献
As a key tool in information theory, Fisher Information has been used to explore the observable behavior of a variety of systems. In particular, recent work has demonstrated its ability to assess the dynamic order of real and model systems. However, in order to solidify the use of this metric for measuring sustainability, it is pertinent that Fisher Information be understood both conceptually and practically. As such, this document has been developed as a guide for interpreting Fisher Information as sustainability metric. Moreover, this study provides details on an analytical and numerical approach to estimating Fisher information based on the evaluation of changes in the system’s trajectory for determining stable and unstable regimes in real systems, which might represent sustainable or unsustainable systems. Using this novel approach, the sustainability of US air quality characterized by criteria pollutants (i.e., lead, carbon monoxide, ozone, nitrogen dioxide, and sulfur dioxide) was assessed from 1980 to 2009. Further, the precision of Fisher Information computations was examined, thereby establishing an alternative procedure for evaluating real systems characterized by noisy and sparse datasets. Throughout this article, the reader is led through the analysis of Fisher Information results with the goal of both providing guidance on the interpretation of Fisher Information and giving a more tactile understanding of the results applied to sustainability assessment. 相似文献
Alkenyl succinic anhydrides (ASA) were obtained by reaction between maleic anhydride and high‐oleic sunflower oil (HOSO) esters. A kinetics study of the maleinization of alkyl esters indicated that the maleinization reaction was second order overall and first order with respect to the individual reactants, and the activation energy was 77.2 ± 3.3 kJ/mol in the investigated temperature range (185–225 °C). These results showed that the cis configuration and the central position of the double bond in HOSO esters facilitate the maleinization of the latter. On the contrary, the length of the linear ester moiety had no influence on the course of the maleinization reaction. Moreover, new evidence demonstrates that there are two different reaction mechanisms: ene‐reaction and addition in allylic position with a 2 : 1 ratio, respectively. This ratio was constant throughout the reaction, thus indicating that these mechanisms are independent. 相似文献
Urban systems have a number of factors (i.e., economic, social, and environmental) that can potentially impact growth, change, and transition. As such, assessing and managing these systems is a complex challenge. While, tracking trends of key variables may provide some insight, identifying the critical characteristics that truly impact the dynamic behavior of these systems is difficult. As an integrated approach to evaluate real urban systems, this work contributes to the research on scientific techniques for assessing sustainability. Specifically, it proposes a practical methodology based on the estimation of dynamic order, for identifying stable and unstable periods of sustainable or unsustainable trends with Fisher Information (FI) metric. As a test case, the dynamic behavior of the City, Suburbs, and Metropolitan Statistical Area (MSA) of Cincinnati was evaluated by using 29 social and 11 economic variables to characterize each system from 1970 to 2009. Air quality variables were also selected to describe the MSA's environmental component (1980-2009). Results indicate systems dynamic started to change from about 1995 for the social variables and about 2000 for the economic and environmental characteristics. 相似文献