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31.
32.
Paracoccidioides brasiliensis, the causative agent of paracoccidioidomycosis (PCM), was first isolated from armadillos from the Amazonian region where the mycosis is uncommon. In the present study, we report on the high incidence of PCM infection in armadillos from a hyperendemic region of the disease. Four nine-banded armadillos (Dasypus novemcinctus) were captured in the endemic area of Botucatu, Sao Paulo, Brazil, killed by manual cervical dislocation and autopsied under sterile conditions. Fragments of lung, spleen, liver, and mesenteric lymph nodes were processed for histology, cultured on Mycosel agar at 37 degrees C, and homogenized for inoculation into the testis and peritoneum of hamsters. The animals were killed from week 6 to week 20 postinoculation and fragments of liver, lung, spleen, testis, and lymph nodes were cultured on brain heart infusion agar at 37 degrees C. Paracoccidioides brasiliensis was isolated from three armadillos both by direct organ culture and from the liver, spleen, lung, and mesenteric lymph nodes of hamsters. In addition, one positive armadillo presented histologically proven PCM disease in a mesenteric lymph node. The three armadillos isolates (Pb-A1, Pb-A2, and Pb-A4) presented thermodependent dimorphism, urease activity, and casein assimilation, showed amplification of the gp43 gene, and were highly virulent in intratesticularly inoculated hamsters. The isolates expressed the gp43 glycoprotein, the immunodominant antigen of the fungus, and reacted with a pool of sera from PCM patients. Taken together, the present data confirm that armadillos are a natural reservoir of P. brasiliensis and demonstrate that the animal is a sylvan host to the fungus. 相似文献
33.
Uchiyama K. Arakawa F. Narita S. Aoki H. Kawasaki I. Matsui S. Yamamoto M. Nakagawa N. Kudo I. 《Micro, IEEE》1993,13(5):12-22
The Gmicro/500, which features a RISC-like dual-pipeline structure for high-speed execution of basic instructions and represents a significant advance for the TRON architecture, is presented. Upwardly-object-compatible with earlier members of the Gmicro series, this microprocessor uses resident dedicated branch buffers to greatly enhance branch instruction execution speed. Its microprograms simultaneously use dual execution blocks to execute high-level language instructions effectively. Fabricated with a 0.6-μm CMOS technology on a 10.9-mm×16-mm die, the chip operates at 50/66 MHz and achieves a processing rate of 100/132 MIPS 相似文献
34.
Hideyuki Yoshimatsu Hitoshi Kawasaki Akiyoshi Osaka 《Journal of Materials Science》1988,23(1):332-336
Zr-Al organometallic compounds have been spray-dried and heated at temperatures 600 to 1400°C to prepare ZrO2-Al2O3 composite powders. The powders consist of balloon-like particles 0.5 to 2 m in diameter with homogeneously dispersed tetragonal ZrO2 grains 0.1 to 0.2 m in diameter. The tetragonal fraction of ZrO2 in the composite powders is higher than that in the powders prepared from sols of Zr(OBun)4 and Al[OCH(CH3)2]3. The fraction is affected by the organofunctional group in the Zr-Al compounds.Zr(OBun)4 = Zr(OC4Hgn)4; Al[OCH(CH3)2]3 = Al(OPri)3. 相似文献
35.
36.
Mixed oxide catalyst prepared by co-precipitating magnesium oxide and calcium oxide showed an excellent activity for the oxidative coupling of methane. The high performances were presumed to arise from the high basicity of the mixed oxide. 相似文献
37.
An investigation was undertaken on the application of dilute chitosan solutions gelled by tyrosinase‐catalyzed reaction with 3,4‐dihydroxyphenethylamine (dopamine). The tyrosinase‐catalyzed reaction with dopamine conferred water‐resistant adhesive properties to the semidilute chitosan solutions. The viscosity of the chitosan solutions increased highly by the tyrosinase‐catalyzed reaction and the subsequent reactions between o‐quinone compounds and chitosan. These highly viscous, gel‐like modified chitosan materials were allowed to spread onto the surfaces of the glass slides, which were tightly lapped together and held them in water. Tensile shear adhesive strength of over 400 kPa was observed for the modified chitosan samples. The increase in the amino group concentration of the chitosan solutions and the molecular mass of the chitosan used effectively led to the increase in adhesive strength of the glass slides. In addition, in the case where the chitosan solution was gelled by the enzymatic reaction with dopamine in the presence of poly(ethylene glycol), adhesive strength sharply increased at shorter reaction times concomitantly with the increase in the viscosity of the chitosan solutions because the tyrosinase activity effectively was retained by poly(ethylene glycol). © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 104: 1818–1827, 2007 相似文献
38.
LaOF-type tetragonal solid solution was found in the system La2O3–MgF2. This phase showed higher anionic conduction than the previously reported cubic solid solution in the system La2O3–CaF2. Electrolysis by Tubandt's method showed that fluoride ion conduction was dominant in this solid solution. The fluoride ions were considered to be easily substituted by oxide ions through cathode reaction in the oxygen atmosphere. 相似文献
39.
Changho Jung Hideyuki Tsuboi Michihisa Koyama Momoji Kubo Ewa Broclawik Akira Miyamoto 《Catalysis Today》2006,111(3-4):322-327
CO adsorption over Pd4 and Pt4 cluster supported by c-ZrO2(1 1 1) and CeO2(1 1 1) catalyst systems was investigated using periodic density functional method in order to clarify the support effect on CO activation. We found that the support increases the CO activation for bridge and three-fold sites but decreases for the atop site. Moreover, it was found that the support changes the site preference for the CO adsorption. Bridge site on both the Pt4/c-ZrO2 and Pt4/CeO2 show larger CO adsorption energies than those on the other sites while the atop site is energetically preferable on isolated Pt4 cluster. c-ZrO2 supported Pd shows the largest CO activation with large charge transfer from the catalyst to the CO molecule. This reveals that ZrO2 supported Pd can be a good catalyst for CO activation because of its higher probability to the three-fold site CO adsorption. We also found that positively charged M4 clusters on the support keep their strong electron-donating properties and have enough charge density to contribute to the activation of an adsorbed CO molecule by a charge transfer. 相似文献
40.
Free primary alcohols in oils and waxes from germs,kernels and other components of nuts,seeds, fruits and cereals 总被引:2,自引:0,他引:2
Kazuko Kawanishi Kumlko Aoki Yohei Hashimoto Akira Matsunobu 《Journal of the American Oil Chemists' Society》1991,68(11):869-872
The composition of free primary alcohols in oils and waxes obtained from the germ, kernel, seed coat, shell and skin (peel)
of various nuts, seeds, fruits and cereals and from the chrysalis of silkworm was examined. These alcohols are usually present
in small amounts, along with large quantities of hydrocarbons, esters and glycerides in oils and waxes. Thus, it is necessary
to remove hydrocarbons, esters and glycerides to analyze the alcohols. We found that preparative reverse-phase thin-layer
chromatography (TLC) was the best way to isolate alcohols from oils and waxes. Gas liquid chromatography (GLC) then detected
hexacosanol, octacosanol and triacontanol in the oils and waxes. Octacosanol usually was the predominant alcohol. Relationships
between the organs from nuts, seeds, fruits and cereals and the contents of octacosanol are suggested. For example, degermed
kernels contained two times more octacosanol than the germ, and the skin coat and shell contained one-half and one-fortieth
the octacosanol of the germ, respectively. 相似文献