WuT3无血清培养上清中单克隆抗体纯化工艺的建立

目的对WuT3杂交瘤细胞生物反应器无血清培养上清中单克隆抗体进行分离纯化,建立中试分离纯化工艺。方法采用两步离子交换层析法结合硫酸铵盐析法分离纯化WuT3单抗。在通过小量试验优化纯化参数后,进行中量试验和中试放大。结果建立的纯化方法可以放大到中试规模,每批投料30000ml培养上清,可收获单抗达1.5g,单抗总回收率大于60%。经分离纯化后,单抗IgG纯度大于95%,采用免疫荧光法检测抗体活性合格。结论建立了一条生物反应器无血清培养杂交瘤细胞大规模分离纯化单抗的工艺路线。     

中碱玻璃纤维表面析碱与强度关系研究

本文通过中碱、无碱玻璃纤维单丝、原丝和方格布水侵蚀下的浸出动力学研究,强度变化研究以及偶联剂处理效果和对玻璃钢强度影响的研究,从而对我国大量工业生产的中碱玻璃纤维的耐水性能、强度性能有了更深刻的认识和评价,为中碱纤维的扩大应用和提高中碱玻璃钢的性能,提供更多的数据资料.     

Limitations of the lead oxide vidicon for dual-energy digital subtraction angiography

Dual-energy digital subtraction angiography (DSA) can be performed, with some modifications, using existing systems designed for conventional DSA work. However, the video camera generally found in such installations, the lead oxide vidicon, places significant limitations on the image quality of dual-energy DSA. If the video camera is operated in a 60 Hz, 266 line progressive scanning mode to maintain a nominal 30 subtraction images/s frame rate, the maximum available X-ray pulse width is limited to 5 ms. The resulting required increase in low-energy kVp reduces the dual-energy signal-to-noise ratio by approximately 40% for cardiac dual-energy DSA imaging. In addition, the performance of the video camera with respect to read-out lag is much more important for dual-energy DSA than conventional single-energy imaging. Measurements show that lag reduces the iodine contrast in dual-energy DSA by up to 20% when a 30 Hz vertical scan mode is used, and by up to 27% when a 60 Hz vertical scan mode is employed. Replacement of the lead oxide vidicon with a CCD camera removes these limitations.     

Metalloorganic solution deposition of ferroelectric PZT films

The metallo-organic solution deposition (MOSD or sol-gel) technique can be used for preparing lead zirconate titanate (PZT) ceramics with a wide range of compositions and crystal structures for electrooptical applications. Film morphology is affected by the stoichiometry of the film, hydrolysis and polymerization of the sol-gel solution, and thermal treatment of the deposited material. The lattice parameter decreases with the amount of titanium in the PZT, in agreement with ceramic data. A slight initial excess of lead in the coating solution is found to improve film morphology. Unlike traditional powder ceramic processing techniques, MOSD permits the growth of small uniform grains that are conducive to achieving consistent electronic and optical properties. Results show that the properties of films prepared by the MOSD process can be tailored to meet the needs of device applications.     

深度卷积神经网络在心音分类方法中的应用

通过分析心音信号对心脏早期的病理状态进行确诊具有重要的意义。提出了一种基于深度卷积神经网络的心音分类方法。将心音信号转化成具有时频特性的梅尔频谱系数（Mel Frequency Spectral Coefficient,MFSC）特征图,将其作为深度卷积神经网络模型的输入;利用深度卷积神经网络对MFSC特征图进行训练,引入中心损失函数建立最优的深度学习模型;测试阶段,先将心音信号转换成多张二维MFSC特征图,然后利用训练好的深度学习模型对其分类,最后利用多数表决原则判断心音信号的类别。针对人工标注的训练样本有限,导致模型训练正确率不高的问题,以心音的二维MFSC特征图为对象分别从时间域和频率域进行随机屏蔽处理进而扩充训练样本。实验结果表明,该方法在PASCAL心音数据集上进行测试,对正常、杂音、早搏三种心音的分类性能明显优于现有最好的方法。     

发电机受潮后的干燥处理

我厂机组属于供热机组当夏季停运后发电机易受潮绝缘降低,本厂由长期运行经验得出一套可行干燥处理方法,对发电机受潮后的处理很有作用.     

现代机械设备制造中自动化技术的运用分析

随着信息时代的发展,各行各业对信息技术的应用逐渐成熟.现代工业领域中的机械设备制造,积极引入了自动化技术,改善了机械设备制造的过程,提高机械生产的效率.自动化技术,优化了机械设备制造的过程,保障生产的自动化,本文在现代机械设备制造中,主要探讨自动化技术的运用.     

以提高定位精度为导向的电子地图应用策略研究

文中针对电子地图定位精度不够,现实的工作和生活中地理状况变化频繁,电子地图更新滞后的现状,提出一种以提高定位精确度的动态共享电子地图应用策略。通过构建实时共享的大容量网络信息平台作为数据支撑,在电子地图的信息点中链接动态图文信息,提高电子地图精确度,最大限度提高电子地图信息的即时性,兼具可视化的特点。该策略可作为电子地图的扩展,在特定行业或区域(如餐饮、旅游)中实施。     

四川盆地德阳——安岳裂陷震旦系灯影组台缘结构特征与勘探方向

基于地震、测井及钻井等资料,对德阳—安岳裂陷及周缘震旦系灯影组台缘展布、结构类型、丘滩体成藏特征进行研究。研究表明：(1)德阳—安岳裂陷及周缘灯影组发育单阶断控型、多阶断控型、缓坡型和多期叠置型共4种台缘结构。(2)高石梯西—威远东地区灯影组二段(简称灯二段)发育单阶断控型台缘,呈近北东东向展布,受断裂和泥岩遮挡,形成断控-岩性圈闭。(3)乐至—蓬莱地区灯二段发育相互独立、多阶断控型台缘,呈北东向排列式展布,受同沉积断裂控制,丘滩体沿断裂上盘加积建隆,被滩间致密带和下寒武统烃源岩多方位遮挡,形成断控-岩性等复合圈闭。(4)威远—资阳地区灯二段发育缓坡型台缘,呈北西向展布,丘滩体多为薄互层连片带状,受上倾方向滩间致密带遮挡,形成岩性圈闭。(5)高石梯—磨溪—盐亭地区,灯影组二段、四段发育叠置型台缘,丘滩体加积叠置呈巨厚台缘建隆,受上倾方向滩间致密带和下寒武统泥岩封盖,在川中古隆起北斜坡形成规模岩性圈闭。蓬莱—中江区块、磨溪北—盐亭地区及威远—资阳地区的灯影组台缘丘滩体规模大,估算资源量1.58×10¹² m³,是四川盆地灯影组未来拓展勘探方向。     

Preparation of Y2O3：Eu^3＋ phosphor by molten salt assisted method

A kind of fine and quasi-spherical Y2O3：Eu^3＋ phosphor was prepared by firing a preparative precursor at 1 200 ℃ for 2 h with the molten salts of Na2CO3, S and NaCl. The precursor was obtained by homogeneous precipitation of yttrium and europium with oxalic acid when using EDTA, citric acid or starch as complexant. The structure and morphology of the phosphors were characterized by XRD and SEM, respectively. The influence of complexing environment, firing temperature and molten salts on formation of the phosphor Y2O3：Eu^3＋ was discussed. The result show that the prepared Y2O3：Eu^3＋ phosphor is of quasi-spherical structure with size of 2-3 μm. Its luminescent intensity is 30% higher than that of the same phosphor prepared by the same procedure but without molten salts, and is 5% higher than that of commercial Y2O3：Eu^3＋ red phosphor.