考虑土分层的搅拌桩支护结构性能研究

将水泥土搅拌桩支护结构及土体视为共同变形的整体,建立了三维有限元模型,选用Drucker-Prager本构模型及非线性增量迭代法,以泉州某基坑为工程原型,利用ANSYS工程计算软件,进行了模型验证,并详细讨论了各支护参数变化时水泥土搅拌桩的位移、应力分布。揭示了地面堆载、墙体厚度、不同土层性质及其分布对搅拌桩位移、应力的影响,墙体位移和应力的关系,分步开挖对搅拌桩位移的影响及合理布置开挖步需考虑的因素。     

涂膜处理和酸处理对提高双孢蘑菇储藏稳定性的研究

为了提高双孢蘑菇(Agaricus bisporus)的储藏稳定性,本研究分别采用了三种不同的方法对双孢蘑菇进行处理,它们分别是:酸处理、涂膜处理和酸与涂膜相结合的处理,然后分别测定了储藏过程中的失重率、硬度、色泽变化、多酚氧化酶(PPO)活力和过氧化物酶活力(POD),探讨了不同处理方法对双孢蘑菇储藏稳定性的影响。结果表明:酸与涂膜相结合的处理方式具有最优的效果,它能弥补酸处理和涂膜处理各自的不足之处,既能降低失重率,保持硬度,又能抑制酶活,更好地保持双孢蘑菇的色泽。      

表层吸收补偿技术在塔里木盆地沙漠区的应用

塔里木盆地沙漠地表对地震信号的吸收衰减导致地震波的能量减弱、分辨率降低;沙漠表层介质的空间变化大,吸收衰减引起地震子波能量和相位的空间变化,如果该变化远大于储层信息的空间变化,会造成储层预测失败。表层吸收补偿技术是一种基于确定性近地表模型的定量分析及衰减补偿技术,通过地震记录的折射波主频转换得到相对衰减系数R值,由谱比法求取表层相对Q值,并利用稳定的吸收补偿算法对表层吸收衰减进行地震波补偿,能合理拓宽地震信号的有效频带并提高地震数据的分辨率和信噪比,同时有效解决近地表空变吸收问题。     

基于电阻型频率选择表面的超宽带紧耦合阵列

针对接地板严重影响紧耦合天线阵列(Tightly Coupled Array,TCA)阻抗带宽的问题,设计了一种基于电阻型频率选择表面(Resistive Frequency Selective Surface,RFSS)的多层结构超宽带TCA,分析了电阻膜方阻及单元间耦合对阻抗带宽的影响,并对其扫描及未扫描时的性能进行了仿真分析.结果表明:通过在天线阵列与接地板之间加载开口谐振环结构的RFSS,可减小接地板反射,抑制天线短路点,显著增加其阻抗带宽,其辐射性能在整个带宽内均较稳定;由于RFSS加入了损耗,天线阵列增益有所降低.该多层结构TCA具有带宽宽、体积小、易共形等特点,在超宽带小型化相控阵天线中有良好应用前景.     

四川传统发酵肉中乳酸菌的分离及发酵特性研究

对四川传统发酵肉中乳酸菌进行分离鉴定,并对其发酵特性进行研究。以四川腊肉为研究对象,采用平板划线法对乳酸菌进行分离纯化,利用16S r DNA的序列测定技术对其鉴定,并对分离菌株在不同温度、p H、Na Cl浓度、亚硝酸盐浓度条件下的生长代谢、产酸能力进行研究,并对发酵产物中游离氨基酸进行测定分析。结果表明:筛选出的一株优势乳酸菌经分子生物学鉴定为戊糖片球菌(Pediococcus pentosaceus)。分离菌株对数生长期为6~18 h;最适发酵温度30℃,发酵p H5.5;对Na Cl及Na NO2耐受能力分别高达10%和150 mg/L;其代谢产物中共测出16种游离氨基酸,共包含5种人体必需氨基酸。      

Hulthén势散射态的解析解

对改良的Manning-Rosen势的径向薛定谔方程任意l波散射态进行解析求解,得到按“K/2π标度”的相移公式和归一化的超几何函数表示的径向波函数。通过对散射振幅在极点解析性质的研究得到束缚态能级, 而特征值的数值结果与先前结果的比较进一步验证了本文结果的精确性。     

Expression Patterns and Gonadotropin Regulation of the TGF-β II Receptor (Bmpr2) during Ovarian Development in the Ricefield Eel Monopterus albus

Bmpr2 plays a central role in the regulation of reproductive development in mammals, but its role during ovarian development in fish is still unclear. To ascertain the function of bmpr2 in ovarian development in the ricefield eel, we isolated and characterized the bmpr2 cDNA sequence; the localization of Bmpr2 protein was determined by immunohistochemical staining; and the expression patterns of bmpr2 in ovarian tissue incubated with FSH and hCG in vitro were analyzed. The full-length bmpr2 cDNA was 3311 bp, with 1061 amino acids encoded. Compared to other tissues, bmpr2 was abundantly expressed in the ovary and highly expressed in the early yolk accumulation (EV) stages of the ovary. In addition, a positive signal for Bmpr2 was detected in the cytoplasm of oocytes in primary growth (PG) and EV stages. In vitro, the expression level of gdf9, the ligand of bmpr2, in the 10 ng/mL FSH treatment group was significantly higher after incubation for 4 h than after incubation for different durations. However, bmpr2 expression in the 10 ng/mL FSH treatment group at 2 h, 4 h and 10 h was significantly lower. Importantly, the expression level of bmpr2 and gdf9 in the 100 IU/mL hCG group had similar changes that were significantly decreased at 4 h and 10 h. In summary, Bmpr2 might play a pivotal role in ovarian growth in the ricefield eel, and these results provide a better understanding of the function of bmpr2 in ovarian development and the basic data for further exploration of the regulatory mechanism of gdf9 in oocyte development.     

Human Vascular Endothelial Cells Promote the Secretion of Vascularization Factors and Migration of Human Skin Fibroblasts under Co-Culture and Its Preliminary Application

The good treatment of skin defects has always been a challenge in the medical field, and the emergence of tissue engineering skin provides a new idea for the treatment of injured skin. However, due to the single seed cells, the tissue engineering skin has the problem of slow vascularization at the premonitory site after implantation into the human body. Cell co-culture technology can better simulate the survival and communication environment of cells in the human body. The study of multicellular co-culture hopes to bring a solution to the problem of tissue engineering. In this paper, human skin fibroblasts (HSFs) and human vascular endothelial cells (HVECs) were co-cultured in Transwell. The Cell Counting Kit 8 (CCK8), Transwell migration chamber, immunofluorescence, Western blot (WB), and real time quantitative PCR (RT-qPCR) were used to study the effects of HVECs on cell activity, migration factor (high mobility group protein 1, HMGB1) and vascularization factor (vascular endothelial growth factor A, VEGFA and fibroblast growth factor 2, FGF2) secretion of HSFs after co-cultured with HVECs in the Transwell. The biological behavior of HSFs co-cultured with HVECs was studied. The experimental results are as follows: (1) The results of cck8 showed that HVECS could promote the activity of HSFs. (2) HVECs could significantly promote the migration of HSFs and promote the secretion of HMGB1. (3) HVECs could promote the secretion of VEGFA and FGF2 of HSFs. (4) The HVECs and HSFs were inoculated on tissue engineering scaffolds at the ratio of 1:4 and were co-cultured and detected for 7 days. The results showed that from the third day, the number of HSFs was significantly higher than that of the control group without HVECs.     

Shifting the pH Optima of (R)-Selective Transaminases by Protein Engineering

Amine transaminases (ATAs) are powerful biocatalysts for the stereoselective synthesis of chiral amines. However, wild-type ATAs usually show pH optima at slightly alkaline values and exhibit low catalytic activity under physiological conditions. For efficient asymmetric synthesis ATAs are commonly used in combination with lactate dehydrogenase (LDH, optimal pH: 7.5) and glucose dehydrogenase (GDH, optimal pH: 7.75) to shift the equilibrium towards the synthesis of the target chiral amine and hence their pH optima should fit to each other. Based on a protein structure alignment, variants of (R)-selective transaminases were rationally designed, produced in E. coli, purified and subjected to biochemical characterization. This resulted in the discovery of the variant E49Q of the ATA from Aspergillus fumigatus, for which the pH optimum was successfully shifted from pH 8.5 to 7.5 and this variant furthermore had a two times higher specific activity than the wild-type protein at pH 7.5. A possible mechanism for this shift of the optimal pH is proposed. Asymmetric synthesis of (R)-1-phenylethylamine from acetophenone in combination with LDH and GDH confirmed that the variant E49Q shows superior performance at pH 7.5 compared to the wild-type enzyme.